Open AccessJournal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
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TLDR
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.Abstract:
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.read more
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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
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“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
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Determination of carbonyl content in oxidatively modified proteins.
Rodney L. Levine,Donita Garland,Cynthia N. Oliver,Adolfo Amici,Isabel Climent,Anke-G. Lenz,Bong-Whan Ahn,Shmuel Shaltiel,Earl R. Stadtman +8 more
TL;DR: This chapter discusses methods to determine carbonyl content in oxidatively modified proteins and quantitated protein-bound pyruvoyl groups through formation of a Schiff base with p-aminobenzoic acid followed by reduction with cyanoborohydride.
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The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells
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References
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels
Journal ArticleDOI
Physiological Studies of Conditional Lethal Mutants of Bacteriophage T4D
Richard H. Epstein,A. Bolle,C. M. Steinberg,E. Kellenberger,E. Boy de la Tour,R. Chevalley,R. S. Edgar,M. Susman,G. H. Denhardt,A. Lielausis +9 more
Journal ArticleDOI
Analysis of C14-labeled proteins by disc electrophoresis
TL;DR: This report presents an alternative procedure involving autoradiography of dried longitudinal gel slices that is relatively uncomplicated and can be used to develop the entire pattern of radioactivity in a gel without significant sacrifice of resolution.