Journal ArticleDOI
Cleavage rate of mouse embryos in vivo and in vitro
Patricia Bowman,Anne McLaren +1 more
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Mouse embryos (Q strain) developing in vivo from the 2-cell to the blastocyst stage showed a constant cell doubling time of about 10 h, and addition of oestrogen to the culture medium increased the diameter of blastocysts but did not increase cell number.Abstract:
Mouse embryos (Q strain) developing in vivo from the 2-cell to the blastocyst stage showed a constant cell doubling time of about 10 h. Embryos cultured in vitro over the same period showed a rate of cleavage which was initially almost as great as in the reproductive tract, but subsequently declined to give a doubling time of about 24 h. Addition of oestrogen to the culture medium increased the diameter of blastocysts but did not increase cell number.read more
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Molecular cues to implantation
Sudhansu K. Dey,Hyunjung Jade Lim,Sanjoy K. Das,Jeff Reese,Bibhash C. Paria,Takiko Daikoku,Haibin Wang +6 more
TL;DR: It is hoped that the forthcoming information will generate new ideas and concepts for a process that is essential for maintaining procreation and solving major reproductive health issues in women.
Journal ArticleDOI
Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors.
Bibhash C. Paria,Sudhansu K. Dey +1 more
TL;DR: Clear evidence is presented that specific growth factors of embryonic and/or reproductive tract origin participate in preimplantation embryo development and blastocyst functions in an autocrine/paracrine manner.
Journal ArticleDOI
Plasminogen activator in early embryogenesis. Enzyme production by trophoblast and parietal endoderm.
TL;DR: The results are consistent with the concept, deveolped from work on other cell types, that plasminogen activator may represent a generalized mechanism for tissue remodeling and cell migration.
Journal ArticleDOI
Sexually dimorphic sterility phenotypes in Hoxa10-deficient mice.
TL;DR: It is suggested that maternal Hoxal0 is required to regulate the expression of a factor that affects the viability of preimplantation embryos and homozygotes are fully viable and show an anterior homeotic transformation of lumbar vertebrae.
Journal ArticleDOI
Human embryo features that influence the success of cryopreservation with the use of 1,2 propanediol
Bruno Lassalle,Bruno Lassalle,Jacques Testart,Jacques Testart,Jean-Paul Renard,Jean-Paul Renard +5 more
TL;DR: Survival was higher for 2-day-old embryos than for 3- day-old babies, and for 2, 4, and 8-cell embryos more than for intermediate-cleavage-stage embryos, and the average viability of all 2-Day-old frozen-thawed embryos can be estimated at 19%.
References
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Journal ArticleDOI
A method for in vitro cultivation of mouse ova from two-cell to blastocyst.
TL;DR: A method for relatively large-scale in vitro experiments on the early cleavage divisions of the mouse embryo has been developed in this laboratory and is now being used in several types of study.
Journal ArticleDOI
Viability and growth of mouse embryos after in vitro culture and fusion
Patricia Bowman,Anne McLaren +1 more
TL;DR: The longer the eggs were maintained in culture, the lower was their viability after transfer, and the lighter were the foetuses derived from them, implying that growth regulation of fused embryos is complete by the 17th day of gestation.
Journal ArticleDOI
Studies on the development of mouse embyros in vitro. III. The effect of fixed-nitrogen source
TL;DR: It was found that two-cell mouse ova would not develop into blastocysts in medium that did not contain a fixed-nitrogen source, and single amino acids were omitted from the medium containing the constituent amino acids of BSA in an attempt to demonstrate an essential amino acid requirement of two- cell mouse Ova.
Journal ArticleDOI
Influence of spermatozoa on the cleavage rate of mouse eggs.
W. K. Whitten,C. P. Dagg +1 more
Journal ArticleDOI
Mouse blastocysts grown in vivo and in vitro: carbon dioxide production and trophoblast outgrowth.
Teresa M. Menke,Anne McLaren +1 more
TL;DR: C02 production by blastocyst cultured from the eight-cell stage in Brinster's medium was compared with that of uterine blastocysts recovered from females 3\m=1/2\days p.c.
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Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors.
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