Cloning of a human parvovirus by molecular screening of respiratory tract samples
Tobias Allander,Martti T. Tammi,Margareta Eriksson,Annelie Bjerkner,Annika Tiveljung-Lindell,Björn Andersson +5 more
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TLDR
It is suggested that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated, and a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples is provided.Abstract:
The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, “the human virome,” can be initiated.read more
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Identification of a novel polyomavirus from patients with acute respiratory tract infections.
Anne M. Gaynor,Michael D. Nissen,David M. Whiley,Ian M. Mackay,Stephen B. Lambert,Guang Wu,Daniel C. Brennan,Gregory A. Storch,Theo P. Sloots,David Wang +9 more
TL;DR: The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.
Journal ArticleDOI
Identification of a Third Human Polyomavirus
Tobias Allander,Kalle Andreasson,Shawon Gupta,Annelie Bjerkner,Gordana Bogdanovic,Mats A. A. Persson,Tina Dalianis,Torbjörn Ramqvist,Björn Andersson +8 more
TL;DR: The identification of a previously unknown polyomvirus provisionally named KI polyomavirus, which is phylogenetically related to other primatepolyomaviruses in the early region of the genome but has very little homology to known polyomVirus in the late region, illustrates how unbiased screening of respiratory tract samples can be used for the discovery of diverse virus types.
Journal ArticleDOI
Respiratory Viral Infections in Infants: Causes, Clinical Symptoms, Virology, and Immunology
TL;DR: In this review, the complete picture from epidemiology and virology to clinical impact and immunology is explored, finding that both the virus and the immune response contribute to damage to the lungs and subsequent disease, and therefore, any prevention or treatment needs to address both of these factors.
Journal ArticleDOI
Metagenomic Pyrosequencing and Microbial Identification
Joseph F. Petrosino,Sarah K. Highlander,Ruth Ann Luna,Ruth Ann Luna,Richard A. Gibbs,James Versalovic +5 more
TL;DR: Both the approach of 16S rDNA amplicon sequencing and the whole-genome sequencing approach may be useful for human metagenomics, and numerous bioinformatics tools are being deployed to tackle such vast amounts of microbiological sequence diversity.
Journal ArticleDOI
Human bocavirus and acute wheezing in children.
Tobias Allander,Tobias Allander,Tuomas Jartti,Shawon Gupta,Shawon Gupta,Hubert G. M. Niesters,Pasi Lehtinen,Riikka üsterback,Tytti Vuorinen,Matti Waris,Annelie Bjerkner,Annelie Bjerkner,Annika Tiveljung-Lindell,Annika Tiveljung-Lindell,Bernadette G. van den Hoogen,Timo Hyypiä,Olli Ruuskanen +16 more
TL;DR: Results suggest a model for bocavirus infection in which high viral loads are potentially associated with respiratory symptoms and low viral loads indicate asymptomatic shedding, and quantitative polymerase chain reaction analysis may be important for additional studies of human bocvirus.
References
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Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
TL;DR: ClUSTAL X is a new windows interface for the widely-used progressive multiple sequence alignment program CLUSTAL W, providing an integrated system for performing multiple sequence and profile alignments and analysing the results.
Journal ArticleDOI
Identification of a new human coronavirus
Lia van der Hoek,Krzysztof Pyrc,Maarten F. Jebbink,Wilma Vermeulen-Oost,Ron J. M. Berkhout,Katja C. Wolthers,Pauline M. E. Wertheim-van Dillen,Jos Kaandorp,Joke Spaargaren,Ben Berkhout +9 more
TL;DR: A new group 1 coronavirus, HCoV-NL63, was identified in a 7-month-old child suffering from bronchiolitis and conjunctivitis as discussed by the authors.
Journal ArticleDOI
WWW-query: an on-line retrieval system for biological sequence banks.
Guy Perrière,Manolo Gouy +1 more
TL;DR: A World Wide Web version of the sequence retrieval system Query: WWW-Query is developed, which allows to query nucleotide sequence banks in the EMBL/GenBank/DDBJ formats and protein sequence Banks in the NBRF/PIR format.
Journal ArticleDOI
Characterization and Complete Genome Sequence of a Novel Coronavirus, Coronavirus HKU1, from Patients with Pneumonia
Patrick C. Y. Woo,Susanna K. P. Lau,Chung-Ming Chu,Kwok-Hung Chan,Hoi-Wah Tsoi,Yi Huang,Beatrice H.L. Wong,Rosana W.S. Poon,James J. Cai,Wei-Kwang Luk,Leo L.M. Poon,Samson S. Y. Wong,Yi Guan,J. S. Malik Peiris,Kwok-Yung Yuen +14 more
TL;DR: The data support the existence of a novel group 2 coronavirus associated with pneumonia in humans, CoV-HKU1, from a 71-year-old man with pneumonia who had just returned from Shenzhen, China.
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