Cloning of human picobirnavirus genomic segments and development of an RT-PCR detection assay.
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TLDR
The view that picobirnaviruses constitute a distinct family of viruses is supported, as they were detected in samples from two outbreaks of gastroenteritis in long-term elder care facilities but were not determined to be the primary pathogen.Citations
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RNA Viral Community in Human Feces: Prevalence of Plant Pathogenic Viruses
Tao Zhang,Mya Breitbart,Wah Heng Lee,Jin-Quan Run,Chia-Lin Wei,Shirlena Wee Ling Soh,Martin L. Hibberd,Edison T. Liu,Forest Rohwer,Yijun Ruan +9 more
TL;DR: The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations—up to 109 virions per gram of dry weight fecal matter, indicating that this plant virus is prevalent in the human population.
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A Novel Bocavirus Associated with Acute Gastroenteritis in Australian Children
Jane Arthur,Geoffrey D. Higgins,Geoffrey P. Davidson,Geoffrey P. Davidson,Rodney C. Givney,Rodney M. Ratcliff +5 more
TL;DR: HBoV2 and HBoV3 are newly discovered bocaviruses, of which H BoV2 is the thirdmost-prevalent virus, after rotavirus and astrovirus, associated with pediatric AGE in this study.
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Evaluation of pepper mild mottle virus, human picobirnavirus and Torque teno virus as indicators of fecal contamination in river water
TL;DR: The high excretion level and dissemination of PMMoV in human sewage and river water suggest thatPMMoV could be a promising indicator of fecal pollution in surface water.
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Eukaryotic Viruses in Wastewater Samples from the United States
TL;DR: A baseline understanding of viruses in raw sewage will enable educated decisions to be made regarding the use of different viruses in water quality assessments, and uncovered previously unknown sequence diversity in human picobirnaviruses.
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Faecal virome of cats in an animal shelter.
TL;DR: The detection of such a diverse collection of viruses shed within a single shelter suggested that such animals experience robust viral exposures, increasing the understanding of the viral diversity in cats and facilitating future evaluation of their pathogenic and zoonotic potentials.
References
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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Rapid and simple method for purification of nucleic acids.
R. Boom,C. J. A. Sol,M. M. M. Salimans,C. L. Jansen,P.M.E. Wertheim-van Dillen,J. van der Noordaa +5 more
TL;DR: A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
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Fast and sensitive silver staining of DNA in polyacrylamide gels
TL;DR: The photochemically derived silver stain of nucleic acids in polyacrylamide gels originally described by Merril et al. was modified to reduce unspecific background staining and increase sensitivity and this improved staining procedure is applied for the routine analysis of complex DNA profiles generated by DNA amplification fingerprinting (DAF).
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Molecular Epidemiology of “Norwalk-like Viruses” in Outbreaks of Gastroenteritis in the United States
TL;DR: Improved molecular techniques to detect NLVs demonstrate that most outbreaks of nonbacterial gastroenteritis in the United States appear to be associated with these viruses and that sequence analysis is a robust tool to help link or differentiate these outbreaks.