Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil

doi:10.1590/S0101-20612014005000015

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Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil

Journal Article•DOI•

Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil

Jonierison Alves Pontis¹, Luiz Antonio Mendonça Alves da Costa¹, Silvio José Reis da Silva², Adriana Flach¹•Institutions (2)

Federal University of Roraima¹, AmeriCorps VISTA²

01 Mar 2014-Food Science and Technology International (Sociedade Brasileira de Ciência e Tecnologia de Alimentos)-Vol. 34, Iss: 1, pp 69-73

TL;DR: The total phenolic and flavonoid content, color, and antioxidant activity were evaluated from ten honey samples from Apis mellifera L. collected from Roraima State, Brazil as mentioned in this paper.

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Abstract: The total phenolic and flavonoid content, color, and antioxidant activity were evaluated from ten honey samples from Apis mellifera L. collected from Roraima State, Brazil. The total phenolic content was determined using the Folin-Ciocalteu reagent and ranged from 250 to 548 mg gallic acid kg –1 of honey. The total flavonoid content was obtained using two methods: total flavones/flavonols were determined with aluminum chloride, and flavonones/dihydroflavonols were determined with 2,4-dinitrophenylhydrazine. The results ranged from 9 to 48.6 mg of quercetine kg –1 of honey and 1805 to 2606 mg of pinocembrin kg –1 of honey, respectively. Antioxidant activity was measured with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavengers, and the results of the IC 50 ranged from 3.17 to 8.79 mg/mL. Statistical analysis demonstrated positive correlations between color intensity, flavone and flavonol content, and phenolic content and negative correlations with antioxidant capacity.

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Biomolecules-mediated synthesis of selenium nanoparticles using Aspergillus oryzae fermented Lupin extract and gamma radiation for hindering the growth of some multidrug-resistant bacteria and pathogenic fungi.

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Farag M. Mosallam, Gharieb S. El-Sayyad, Rasha M. Fathy, Ahmed I. El-Batal

01 Sep 2018-Microbial Pathogenesis

TL;DR: Depending on the unique characteristics, and the novelty in biosynthesis process of SeNPs, it must be candidates in biomedicine, prevent food spoilage, cosmetics, and pharmaceutics as green antimicrobial agent.

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Journal Article•DOI•

Antioxidant and anticancer activities of chamomile (Matricaria recutita L.).

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Bayan Al-Dabbagh¹, Ismail A. El-Haty¹, Mohamed Elhaw¹, Chandraprabha Murali¹, Ameera Al Mansoori¹, Basma Awad¹, Amr Amin¹ - Show less +3 more•Institutions (1)

United Arab Emirates University¹

03 Jan 2019-BMC Research Notes

TL;DR: The mode of action of the extract was assessed as a cancer preventive agent and its ability to regulate tumor angiogenesis by down regulating in a dose dependent manner the expression of some proteins involved in the process was reported.

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Abstract: The present study aimed at determining the antioxidant activity, total phenols and flavonoids and to evaluate the antiproliferative activity of ethanolic extract of Matricaria recutita L. (chamomile). The antioxidant activities were measured using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The total phenolic content was measured by the Folin–Ciocalteu assay. The flavonoid content was determined using the aluminum chloride method. The MTT assay was used to estimate the antiproliferative activities against human hepatoma (HepG2) cancer cell line. We assessed the mode of action of the extract as a cancer preventive agent and reported its ability to regulate tumor angiogenesis by down regulating in a dose dependent manner the expression of some proteins involved in the process. The percentage inhibition of DPPH scavenging activity was dose-dependent ranging between (94.8% ± 0.03) at 1.50 mg/mL and (84.2% ± 0.86) at 0.15 mg/mL. It showed high polyphenols (21.4 ± 0.327 mg GAE/g) and high flavonoids content (157.9 ± 2.22 mg QE/g). Effect of extract was investigated against HepG2 cells. A dose-dependent reduction in cell viability was recorded in cells treated with the extract. The IC50 was ~ 300 µg/mL. It significantly inhibited the level of important prerequisite angiogenesis markers both in HepG2 cells and ex vivo.

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83 citations

Journal Article•DOI•

Antioxidant and anticancer activities of Trigonella foenum-graecum, Cassia acutifolia and Rhazya stricta

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Bayan Al-Dabbagh¹, Ismail A. El-Haty¹, Ala’a Al Hrout¹, Reem Al Sakkaf¹, Raafat El-Awady², S. Salman Ashraf¹, Amr Amin³, Amr Amin¹ - Show less +4 more•Institutions (3)

United Arab Emirates University¹, University of Sharjah², Cairo University³

22 Aug 2018-BMC Complementary and Alternative Medicine

TL;DR: R. stricta is introduced as a potentially useful anti-metastatic agent and a novel potential anti-tumour agent for hepatocellular carcinoma (HCC) treatment and is found to be consistent with their antioxidant activity.

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Abstract: Here, we determined in vitro antioxidant activity, total phenols and flavonoids and evaluated antiproliferative activity of three medicinal plant extracts: Trigonella foenum-graecum (Fenugreek), Cassia acutifolia (Senna) and Rhazya stricta (Harmal). The leaves of the three medicinal plants were extracted with 70% ethanol. Antioxidant activities of the extracts were determined by using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay. Total flavonoid and phenolic contents were determined using colorimetric assays. MTT assay was used to estimate the antiproliferative activities of the extracts against human hepatoma (HepG2) cancer cell line. In addition, the effects of R. stricta extract on cell cycle, colony formation, and wound healing of HepG2 cells and tube formation of HUVEC cells were assessed. Percentage inhibition of DPPH scavenging activity were dose-dependent and ranged between (89.9% ± 0.51) and (28.6% ± 2.07). Phenolic contents ranged between (11.5 ± 0.013) and (9.7 ± 0.008) mg GAE/g while flavonoid content ranged between (20.8 ± 0.40) and (0.12 ± 0.0.01) mg QE/g. Antiproliferative results of the extracts were found to be consistent with their antioxidant activity. Among the extracts evaluated, that of R. stricta showed the best antioxidant, antiproliferative and antimetastatic activities at low concentration. It also inhibited the colony-formation capacity of HepG2 cells and exhibited antiangiogenic activity. Cell cycle analysis showed significant arrest of cells at G2/M phase 12 and 48 h after treatment and significant arrest at G1/S phase after 24 h of treatment. Consistent data were observed in western blot analysis of protein levels of Cdc2 and its cyclin partners. These findings introduce R. stricta as a potentially useful anti-metastatic agent and a novel potential anti-tumour agent for hepatocellular carcinoma (HCC) treatment.

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80 citations

Journal Article•DOI•

Color, flavonoids, phenolics and antioxidants of Omani honey.

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Mohamed Al-Farsi, Abeer Al-Amri, Ahlam Al-Hadhrami, Sharifa Al-Belushi

01 Oct 2018-Heliyon

TL;DR: The Omani honey was rich in color and phenolics compare to other Honey producing regions in the Sultanate of Oman and regarded as a good source of antioxidants to the human diet.

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69 citations

Cites background or methods from "Color, phenolic and flavonoid conte..."

...DPPH method was chosen to measure antioxidants in honey because it’s easy, precise and accurate method (Pontis et al., 2014)....
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...…Sidr and multifloral color were similar to values reported for Algerian honey which ranged between 31 and 198 mm (Rebia and Lanez, 2014), Brazilian honey which ranged between 31 and 166 mm (Pontis et al., 2014) and for Argentinian honey color ranged between 40.7 and 140 mm (Cabrera et al., 2017)....
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...8 mg/kg (Ferreira et al., 2009) and Brazil 250e548 mg/kg (Pontis et al., 2014)....
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...Pontis et al. (2014) reported color: phenolics correlation (.967), color: antioxidants ( 0.800) and phenolics: antioxidants ( 0.752)....
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Journal Article•DOI•

Salvadora persica (Miswak): Antioxidant and Promising Antiangiogenic Insights

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Bayan Al-Dabbagh, Ismail A. El-Haty, Chandraprabha Murali, Ahmed Al Madhoon, Amr Amin - Show less +1 more

24 May 2018-American Journal of Plant Sciences

TL;DR: In vitro antioxidant activity, total phenols and flavonoids and antiproliferative activity of the extract of S. persica (Miswak) were determined and found to be consistent with their antioxidant activity.

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Abstract: The use of plants to improve dental health and to promote oral hygiene has been practiced for centuries and persists in several communities throughout the world. Salvadora persica (Miswak) has a wide geographic distribution. The ancient Arabs had the habit of using it to whiten and polish the teeth. Here, we determined in vitro antioxidant activity, total phenols and flavonoids and evaluated antiproliferative activity of the extract of S. persica (Miswak). The MTT assay was used to estimate the antiproliferative activities of the extract against human hepatoma (HepG2) cancer cell line. Inhibition percentage of DPPH scavenging activity was dose-dependent and ranged from (30.7% ± 0.62) to (5.89% ± 0.98). The phenolic content was (2.7 ± 0.11) mg GAE/g while the flavonoid content was (2.70 ± 0.45) mg QE/g. Antiproliferative results of the extracts were found to be consistent with their antioxidant activity. Our extract also exhibited clear antiangiogenic activity. These findings introduce S. persica as the useful and novel potential anti-tumor agent for hepatocellular carcinoma (HCC) treatment.

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55 citations

Cites background from "Color, phenolic and flavonoid conte..."

...This reagent reacts with phenolic compounds and gives a blue color complex that absorbs radiation at 765 nm and allows quantification [31]....
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References

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Journal Article•DOI•

Estimation of total flavonoid content in propolis by two complementary colorimetric methods

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Chia-Chi Chang, Ming-Hua Yang, Hwei-Mei Wen, Jiing-Chuan Chern

14 Jul 2020-Journal of Food and Drug Analysis

TL;DR: In this article, two complementary colorimetric methods, aluminum chloride method and 2,4-dini trophenylhydrazine method, were used to determine the real content of total flavonoids in propolis.

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3,899 citations

Journal Article•DOI•

Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method

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Luciana Lopes Mensor¹, Fábio de Sousa Menezes¹, Gilda Guimarães Leitão¹, Alexandre da Silva Reis¹, Tereza Cristina dos Santos¹, Cintia S. Coube¹, Suzana G. Leitão¹ - Show less +3 more•Institutions (1)

Federal University of Rio de Janeiro¹

01 Mar 2001-Phytotherapy Research

TL;DR: As a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC50 values than the other plant extracts, among the partitions, the more polar ones (ethyl acetate and n‐butanol) are those that generally have higher antioxidant activity (AA).

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Abstract: Brazilian plant extracts belonging to 16 species of 5 different families (71 extracts) were tested against the stable DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free-radical. The ability to scavenge DPPH radical was measured in these experiments by the discoloration of the solution. Ginkgo biloba and rutin, commonly used as antioxidants for medical purposes, were used as standards. Based on our results, we can say that as a general rule the ethanol extracts of plants belonging to the Verbenaceae family showed lower EC50 values than the other plant extracts. Among the partitions, the more polar ones (ethyl acetate and n-butanol) are those that generally have higher antioxidant activity (AA). Copyright © 2001 John Wiley & Sons, Ltd.

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1,986 citations

Journal Article•DOI•

Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity.

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Aline Meda¹, Charles Euloge Lamien¹, Marco Romito, Jeanne Millogo¹, Odile Germaine Nacoulma¹ - Show less +1 more•Institutions (1)

University of Ouagadougou¹

01 Jul 2005-Food Chemistry

TL;DR: In this paper, several honey samples (27) from Burkina Faso were analyzed to determine their total phenolic, flavonoid and proline contents as well as their radical scavenging activity.

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1,850 citations

"Color, phenolic and flavonoid conte..." refers background or methods in this paper

...The total flavonoid content is generally determined using a spectrophotometric assay based on the formation of an aluminum chloride complex, which has been applied for quantification of flavones and flavonols in honey (Meda et al., 2005; Liberato et al., 2011)....
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...…Federal de Roraima – UFRR, Campus Paricarana, Boa Vista, RR, Brasil, e-mail: aflach@gmail.com 2 Instituto Federal de Educação, Ciência e Tecnologia de Roraima – IFRR, Campus Amajari, Amajari, RR, Brasil 3 Universidade Estadual de Roraima – UERR, Boa Vista, RR, Brasil *Corresponding author...
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Analytical, Nutritional and Clinical Methods Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey

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Jasna Bertoncelj, Mojca Jamnik, Terezija Golob

01 Jan 2007

TL;DR: The results of the study showed that total phenolic content, antioxidant activity, and color parameters differ widely among different honey types as discussed by the authors, and the relationship between the parameters analysed were found to be statistically significant (p < 0.05).

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Abstract: Honey samples from the seven most common honey types in Slovenia were screened for total phenolic content by the modified Folin– Ciocalteu method, for potential antioxidant activity using the ferric reducing antioxidant power (FRAP) assay and by the 1,1-diphenyl-2picrylhydrazyl (DPPH) method for antiradical activity. In addition the colour characteristics of honey samples were analysed. The results of the study showed that total phenolic content, antioxidant activity and colour parameters differ widely among different honey types. Phenolic content expressed as gallic acid equivalent ranged from 44.8 mg/kg in acacia honey to 241.4 mg/kg in fir honey. Antioxidant activity was the lowest in the brightest acacia and lime honeys and the highest in darker honeys, namely fir, spruce and forest. The colour of the Slovenian honeys, analysed in this study was very variable and ranged from pale yellow to dark brown. Correlations between the parameters analysed were found to be statistically significant (p < 0.05). 2007 Elsevier Ltd. All rights reserved.

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566 citations

Journal Article•DOI•

Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey

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Jasna Bertoncelj¹, Urška Doberšek¹, Mojca Jamnik¹, Terezija Golob¹•Institutions (1)

University of Ljubljana¹

01 Jan 2007-Food Chemistry

TL;DR: The results of the study showed that total phenolic content, antioxidant activity, and color parameters differ widely among different honey types as mentioned in this paper, and the relationship between the parameters analysed were found to be statistically significant (p < 0.05).

...read moreread less

556 citations

"Color, phenolic and flavonoid conte..." refers background or methods in this paper

...An important parameter of honey is its color, which reflects the floral source (Bertoncelj et al., 2007) and can be determined by spectrophotometric measurement of the absorbance of a 50% honey solution at 635 nm and classified according to the Pfund scale (Ferreira et al....
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...A colorimetric assay using the Folin-Ciocalteu reagent is often referenced in the literature for the determination of phenolic compounds (Bertoncelj et al., 2007; Socha et al., 2009; Ferreira et al., 2009; Beretta et al., 2005; Kaškonienė et al., 2009)....
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...Statistical analysis of the phenolic content, color, and antioxidant activity has demonstrated positive correlations (Bertoncelj et al., 2007)....
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...Phenolic acids and flavonoids are the main antioxidants in this apiary product (Bertoncelj et al., 2007; Socha et al., 2009)....
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...The IC50 values were calculated using linear regression of plots where the abscissa represented the concentration of the honey solution and the ordinate was the percent of antioxidant activity (Bertoncelj et al., 2007; Socha et al., 2009; Ferreira et al., 2009; Mesor et al., 2001; Baltrušaitytė et al., 2007)....
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