Community-acquired methicillin-resistant staphylococcus aureus carrying panton-valentine leukocidin genes: worldwide emergence
François Vandenesch,Timothy S. Naimi,Mark C. Enright,Gerard Lina,Graeme R. Nimmo,Helen Heffernan,Nadia Liassine,Michèle Bes,Timothy Greenland,Reverdy Me,Jerome Etienne +10 more
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TLDR
Analysis of CA-MRSA isolates from the United States, France, Switzerland, Australia, New Zealand, and Western Samoa indicated distinct genetic backgrounds associated with each geographic origin, although predominantly restricted to the agr3 background.Abstract:
Infections caused by community-acquired (CA)-methicillin-resistant Staphylococcus aureus (MRSA) have been reported worldwide. We assessed whether any common genetic markers existed among 117 CA-MRSA isolates from the United States, France, Switzerland, Australia, New Zealand, and Western Samoa by performing polymerase chain reaction for 24 virulence factors and the methicillinresistance determinant. The genetic background of the strain was analyzed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The CAMRSA strains shared a type IV SCCmec cassette and the Panton-Valentine leukocidin locus, whereas the distribution of the other toxin genes was quite specific to the strains from each continent. PFGE and MLST analysis indicated distinct genetic backgrounds associated with each geographic origin, although predominantly restricted to the agr3 background. Within each continent, the genetic background of CA-MRSA strains did not correspond to that of the hospital-acquired MRSA.read more
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Involvement of Panton-Valentine Leukocidin—Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia
Gerard Lina,Yves Piémont,Florence Godail-Gamot,Michèle Bes,Marie Odile Peter,Valérie Gauduchon,François Vandenesch,Jerome Etienne +7 more
TL;DR: Panton-Valentine leukocidin genes were detected in 93% of strains associated with furunculosis and in 85% of those associated with severe necrotic hemorrhagic pneumonia (all community-acquired), and it appears that PVL is mainly associated with nec rotic lesions involving the skin or mucosa.
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Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients
Yves Gillet,Bertrand Issartel,Philippe Vanhems,Jean-Christophe Fournet,Gerard Lina,Michèle Bes,François Vandenesch,Yves Piémont,Nicole Brousse,Daniel Floret,Jerome Etienne +10 more
TL;DR: PVL-producing S aureus strains cause rapidly progressive, haemorrhagic, necrotising pneumonia, mainly in otherwise healthy children and young adults, and has a high lethality rate.
Journal ArticleDOI
Multiplex PCR Strategy for Rapid Identification of Structural Types and Variants of the mec Element in Methicillin-Resistant Staphylococcus aureus
TL;DR: The development, validation, and application of a multiplex PCR strategy that allows quick presumptive characterization of the mec element types based on the structural features that were shown to be typical of mec elements carried by several MRSA clones are reported.