Comparación de la cromatografía líquida de alta resolución frente al enzimoinmunoensayo de micropartículas para la determinación de sirolimus en trasplante renal
R Asensi Diez,M.P. Quesada Sanz,E. Valverde Molina,E. de Ramón Garrido,J. Gómez Maldonado,I Muñoz Castillo +5 more
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TLDR
Investigation of whether hemoglobin, hematocrit, and tome from kidney transplantation influence results obtained using the immune-enzymatic technique renders enzyme immunoassay a good alternative for the measurement of sirolimus levels in whole blood finding its correlation with chromatography is acceptable.About:
This article is published in Farmacia Hospitalaria.The article was published on 2006-05-01 and is currently open access. It has received 4 citations till now. The article focuses on the topics: Microparticle Enzyme Immunoassay.read more
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Journal ArticleDOI
Development and Validation of an HPLC Method for the Analysis of Sirolimus in Drug Products
TL;DR: The rapid and sensitive developedRP-HPLC method can be used for the routine analysis of sirolimus such as dissolution and stability assays of pre- and post-marketed dosage forms.
Journal ArticleDOI
Determination of blood sirolimus concentrations in liver and kidney transplant recipients using the Innofluor® fluorescence polarization immunoassay: Comparison with the microparticle enzyme immunoassay and high-performance liquid chromatography-ultraviolet method
TL;DR: The Innofluor® FPIA is a valid alternative with an analogous performance to the MEIA for the therapeutic monitoring of sirolimus and no significant effect was found for siro Limus clearance or the blood hematocrit on the relationship between the results produced by both immunoassays and HPLC.
Journal ArticleDOI
A microparticle enzyme immunoassay to measure sirolimus
TL;DR: It is concluded that, despite the analytical bias, this immunoassay offers a viable alternative to the use of HPLC and would be an assay suitable for implementing at local centers.
References
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Statistical methods for assessing agreement between two methods of clinical measurement.
TL;DR: An alternative approach, based on graphical techniques and simple calculations, is described, together with the relation between this analysis and the assessment of repeatability.
Journal ArticleDOI
Analytical Methods Validation: Bioavailability, Bioequivalence, and Pharmacokinetic Studies
Vinod P. Shah,Kamal K. Midha,Shrikant Dighe,Iain J. McGilveray,Jerome P. Skelly,Avraham Yacobi,Thomas Layloff,C. T. Viswanathan,C. Edgar Cook,R. D. McDowall,Kenneth A. Pittman,Sidney Spector +11 more
TL;DR: The report presents the assessment of the major agreements and issues discussed at the conference onAnalytical Methods Validation to provide guiding principles for validation of analytical methods used in bioavailability, bioequivalence, and pharmacokinetics studies in humans and animals.
Journal ArticleDOI
Calculating correlation coefficients with repeated observations: Part 2—correlation between subjects
J M Bland,Douglas G. Altman +1 more
TL;DR: In earlier Statistics Notes1 2 the authors commented on the analysis of paired data where there is more than one observation per subject and it can be highly misleading to analyse such data by combining repeated observations from several subjects and then calculating the correlation coefficient as if the data were a simple sample.
Journal ArticleDOI
Statistics notes: Calculating correlation coefficients with repeated observations: Part 1—correlation within subjects
Martin Bland,Douglas G. Altman +1 more
TL;DR: The authors pointed out that it could be highly misleading to analyse such data by combining repeated observations from several subjects and then calculating the correlation coefficient as if the data were a simple sample, as shown in table I.