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Journal Article

Comparative haematological and serum biochemical analysis of catfishes Clarias batrachus (Linnaeus, 1758) and Heteropneustes fossilis (Bloch, 1794) with respect to sex

01 Dec 2014-Journal of entomology and zoology studies (AkiNik Publications)-Vol. 2, Iss: 6, pp 191-197
TL;DR: The haematological and serum biochemical parameters of two catfishes Heteropneustes fossilis and Clarias batrachus of Odisha were investigated and compared using One-Way Analysis of Variance (ANOVA) followed by Turkey’s pair wise comparison tests.
Abstract: Aim of this study was to investigate the haematological and serum biochemical parameters of two catfishes Heteropneustes fossilis (Bloch, 1794) and Clarias batrachus (Linnaeus, 1758) of Odisha. In this study, the estimated values were compared between the two species of catfishes as well as with respect to sex. Haematological parameters such as haemoglobin (Hb), total erythrocyte count (TEC), total leucocyte count(TLC), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), and mean corpuscular haemoglobin Concentration (MCHC) and the serum biochemical parameters like protein, albumin, globulin, glucose and cholesterol were measured and compared between and within the species as well as with respect to sex using One-Way Analysis of Variance (ANOVA) followed by Turkey’s pair wise comparison tests. All haematological parameters differ significantly (p<0.001) between C. batrachus and H. fossilis except PCV and MCV. Except albumin all biochemical parameters differ significantly between C. batrachus and H. fossilis.
Citations
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Journal ArticleDOI
02 May 2018
TL;DR: Findings from this study provide a better understanding of the influences of divergent environmental conditions and feeding habits on fish blood parameters.
Abstract: Haematological parameters are valuable indicators of fish health status. This study is aimed to provide baseline data of the blood profile of two teleost fish species living in different environmen...

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TL;DR: The aim of the present study is to establish and compare baseline information about the hematological profile of the two snow trout fish species using standard methodology and light microscopy, and to provide essential tools for physiologists and pathologists in monitoring the health status of theseFish species in particular and fish species in general.
Abstract: The aim of the present study is to compare the hematological parameters of two snow trout fish species, Schizopyge plagiostomus and Schizopyge niger, inhabiting two different habitats, to e...

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Journal ArticleDOI
TL;DR: In this article, the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings was introduced, and one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish.
Abstract: Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline’s values of marine and freshwater fishes. Recently, the use of hematology and biochemistry of fishes in toxicology, aquaculture, environmental pollution, feeding, and antioxidants studies has been increased. In this study we introduced the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings. Hemato-biochemical parameters were widely used in fish toxicological studies. Many researches have used the protein electrophoresis as a valid tool to determining intra and inter-specific variation among species. Protein profile was extensively used in determining the health of fish, as indicators of anemia or other diseases provide information about the existence of the disease, and in the diagnosis of disease. So, to carry out the aim of this study, we reported one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish with shedding the light on the importance of hematological and biochemical parameters in fish toxicological studies.

20 citations


Cites background from "Comparative haematological and seru..."

  • ...There is growing interest in the study of haemato-biochemical parameters and these parameters are regarded as important for aquaculture purposes [3]....

    [...]

Journal ArticleDOI
TL;DR: Five experimental diets were formulated to evaluate the effects of dietary enzymatically hydrolyzed tuna by-product on growth, non-specific immune responses, and hematology of juvenile rainbow trout and indicated that the optimum level of dietary enzyme to replace fishmeal could replace greater than 29.7% but less than 37.5% in rainbow trout.
Abstract: Five experimental diets were formulated to evaluate the effects of dietary enzymatically hydrolyzed tuna by-product on growth, non-specific immune responses, and hematology of juvenile rainbow trout (Oncorhynchus mykiss). A basal diet with 50% of fishmeal was used as control (CON) and four other diets replaced 12.5% (TBB12.5), 25% (TBB25), 37.5% (TBB37.5), and 50% (TBB50) of fish meal in the CON diet. Juvenile rainbow trout (4.87 ± 0.05 g) were randomly distributed into 15 tanks (50 L) and fed 3–4% of wet body weight two times a day. At the end of 7 weeks of feeding trial, weight gain, specific growth rate, feed efficiency, and protein efficiency ratio of fish fed CON diet were significantly higher than those of fish fed TB50 diet (P 0.05). There were no significant differences in GPT levels among fish fed CON, TBB12.5, TBB25, and TBB37.5 diets. Also, there were no significant differences in lysozyme, superoxide dismutase, glucose, and total protein levels in all experimental diet (P > 0.05). The broken-line analysis indicated that the minimum dietary level of enzymatically hydrolyzed tuna by-product to replace fishmeal could be 29.7% in rainbow trout. These results indicated that the optimum level of dietary enzymatically hydrolyzed tuna by-product could replace greater than 29.7% but less than 37.5% of fishmeal in juvenile rainbow trout diet.

12 citations


Additional excerpts

  • ...(Acharya and Mohanty 2014)....

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References
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Book
01 Jan 1974
TL;DR: The objective is to establish an experimental procedure and show direct AFM progression from EMT to EMT using a simple, straightforward, and reproducible procedure.
Abstract: Pathologic basis of disease , Pathologic basis of disease , کتابخانه دیجیتالی دانشگاه علوم پزشکی و خدمات درمانی شهید بهشتی

5,162 citations


"Comparative haematological and seru..." refers result in this paper

  • ...The mean value of RBC differed significantly (p 0.001) between male C. batrachus and female H. fossilis....

    [...]

  • ...Within first 2h after each extraction, the blood samples were processed for Haemoglobin (Hb), total erythrocyte count (TEC/RBC), total leukocyte count (TLC/WBC) and packed cell volume (PCV) as follows: Haemoglobin concentration (Hb) was measured by Sahli’s acid haematin method [14]....

    [...]

  • ...RBC and WBC were determined using a Neubauer haemocytometer....

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  • ...The value of RBC is higher in male than female in both the fishes but it shows significant difference in H. fossilis (Fig....

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  • ...The RBC content of this investigation for male and female C. batrachus is 2.97±0.07 and 2.55±0.10 (106mm-3) and it is lower than that H. fossilis for both male and female are3....

    [...]

Journal ArticleDOI
TL;DR: The use of haematological values in assessing sublethal concentrations of environmental pollutants and the possibility of chromosomal changes are considered.
Abstract: A review is made of some selected literature regarding the use of haematological techniques in freshwater fish pathology. Comparisons are drawn with the techniques already widely used in human pathology for the assessment of health and for aid in diagnosis of various diseases and conditions. The need for information on normal values, and on the conditions under which these were formulated, and the factors affecting them is stressed. The use of haematological values in assessing sublethal concentrations of environmental pollutants and the possibility of chromosomal changes are considered.

394 citations


"Comparative haematological and seru..." refers background in this paper

  • ...Changes in haematological parameters depend upon the aquatic biotope, fish species, age, and sexual maturity and health status [8-10]....

    [...]

Journal ArticleDOI
TL;DR: The cultured sturgeon did not seem susceptible to crowding and prolonged handling stress, since neither the serum cortisol and glucose levels nor the other blood parameters were affected by these stressors.
Abstract: Data on the concentrations of some blood constituents of captive Adriatic sturgeon, Acipenser naccarii, a primitive bony fish, are reported. Serum osmolality, Na+, K+, Cl−, Ca2+, cortisol, glucose and total protein concentrations were measured. The effects of anaesthesia, temperature, crowding and prolonged handling stress were tested on a group of 12 4-year-old sturgeons sampled repeatedly. The anaesthetic dose of MS 222 (140 mg l−1) induced significant osmolality elevation in the sturgeon. After exposure to colder temperature (17 versus 25°C), cortisol and Cl− concentrations significantly decreased. The cultured sturgeon did not seem susceptible to crowding and prolonged handling stress, since neither the serum cortisol and glucose levels nor the other blood parameters were affected by these stressors. Results are compared with the few available data on other chondrostean fish and with those on teleosts.

183 citations


"Comparative haematological and seru..." refers background in this paper

  • ...These should be as close as possible to normal values of various blood components considered as reliable descriptors of healthy fish under natural conditions [11]....

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  • ...For this reason, blood parameters are increasingly used as indicators of the physiological condition or sub-lethal stress response in fish to endogenous or exogenous changes [11-12]....

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Journal ArticleDOI
TL;DR: It is impossible to say whether the differences that were observed between Kamloops and these other varieties were due to strain alone, since other variables were present, but some problems associated with establishing normal ranges for these parameters in fish are discussed.
Abstract: Fifty rainbow trout of the Kamloops strain were examined for 12 haematological parameters: erythrocyte sedimentation rate, haemoglobin, packed cell volume, erythrocyte count, erythrocyte diameters, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, leukocyte count, differential leukocyte count, plasma total protein and plasma glucose concentration. The fish had been held under known environmental and dietetic conditions, and at the time of sampling were 14 months old. The majority of results for erythrocyte sedimentation rate, haemoglobin, packed cell volume, erythrocyte count, erythrocyte diameters, total protein and differential leukocyte count fell within narrow ranges. The total leukocyte counts and glucose levels were more widely spread. The results are discussed and compared with those already published for Idaho and Shasta strains. It is impossible to say whether the differences that were observed between Kamloops and these other varieties were due to strain alone, since other variables were present. Some problems associated with establishing normal ranges for these parameters in fish are discussed.

170 citations

Book ChapterDOI
TL;DR: Thyroid hormones are elevated when fish are under conditions favorable for somatic growth and lowered when conditions are unfavorable, and the main circulating catecholamines in teleosts are adrenaline and noradrenaline, which originate predominantly from the chromaffin tissue, mostly in the head kidney and postcardinal veins.
Abstract: Publisher Summary This chapter discusses the chemical properties of the blood of fishes. The levels of many plasma constituents exhibit daily variations that can modify and complicate any analysis of the influence of environmental factors. The timing of gonadrotropin release and steroidogenesis in temperate zone teleosts is primarily controlled by variations in temperature and photoperiod. Salmonids, spawning mainly in autumn and winter, are cued primarily by decreasing day length, whereas cyprinids, spawning in spring and summer, are more dependent on increasing temperature. Arginine vasotocin from the pars nervosa circulates in the blood of teleosts. It has cardiovascular effects, although its status as a hormone and its role in osmoregulation are uncertain. Thyroid hormones are elevated when fish are under conditions favorable for somatic growth and lowered when conditions are unfavorable. The main circulating catecholamines in teleosts are adrenaline and noradrenaline, which originate predominantly from the chromaffin tissue, mostly in the head kidney and postcardinal veins The circulating catecholamines in cyclostomes and chrondrichthyes are identical to those of teleosts.

142 citations