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Journal ArticleDOI

Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts

01 Sep 2006-Journal of Food Composition and Analysis (JOURNAL OF FOOD COMPOSITION AND ANALYSIS)-Vol. 19, Iss: 6, pp 669-675
TL;DR: Guava fruit extracts were analyzed for antioxidant activity measured in methanol extract and dichloromethane extract (AOAD), ascorbic acid, total phenolics, and total carotenoids contents.
About: This article is published in Journal of Food Composition and Analysis.The article was published on 2006-09-01. It has received 2737 citations till now. The article focuses on the topics: ABTS & Ascorbic acid.
Citations
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Journal ArticleDOI
TL;DR: A significant relationship between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of these plants.
Abstract: Aqueous extracts of 30 plants were investigated for their antioxidant properties using DPPH and ABTS radical scavenging capacity assay, oxygen radical absorbance capacity (ORAC) assay, superoxide dismutase (SOD) assay, and ferric reducing antioxidant potential (FRAP) assay. Total phenolic content was also determined by the Folin−Ciocalteu method. Antioxidant properties and total phenolic content differed significantly among selected plants. It was found that oak (Quercus robur), pine (Pinus maritima), and cinnamon (Cinnamomum zeylanicum) aqueous extracts possessed the highest antioxidant capacities in most of the methods used, and thus could be potential rich sources of natural antioxidants. These extracts presented the highest phenolic content (300−400 mg GAE/g). Mate (Ilex paraguariensis) and clove (Eugenia caryophyllus clovis) aqueous extracts also showed strong antioxidant properties and a high phenolic content (about 200 mg GAE/g). A significant relationship between antioxidant capacity and total phe...

1,358 citations

Journal ArticleDOI
TL;DR: In this paper, the 50 most popular antioxidant-rich fruits, vegetables and beverages in the US diet were identified and analyzed for their antioxidant capacities, total phenolics and flavonoids content.

1,131 citations

Journal ArticleDOI
TL;DR: Rosemary extract was found to have a higher phenolic content than blackseed essential oil, which was utilised in explaining the higher antioxidant activity of rosemary extract.

661 citations

Journal ArticleDOI
TL;DR: In this article, Andean blackberry, capuli cherry peel and banana passion fruit were analysed for total soluble phenolic compounds content and for antioxidant capacity, using three different methods (DPPH, FRAP and ABTS + ).

634 citations

Journal ArticleDOI
TL;DR: Oxygen radical antioxidant capacity and trolox equivalent antioxidant capacity (TEAC) assays were compared to estimate the total antioxidant capacity of orange juice, milk, and an orange juice-milk beverage as discussed by the authors.

518 citations

References
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01 Feb 1980

47,967 citations

Journal ArticleDOI
TL;DR: The antiradical properties of various antioxidants were determined using the free radical 2,2-Diphenyl-1-picrylhydrazyl (DPPH*) in its radical form as discussed by the authors.
Abstract: The antiradical activities of various antioxidants were determined using the free radical, 2,2-Diphenyl-1-picrylhydrazyl (DPPH*). In its radical form. DPPH* has an absorption band at 515 nm which dissappears upon reduction by an antiradical compound. Twenty compounds were reacted with the DPPH* and shown to follow one of three possible reaction kinetic types. Ascorbic acid, isoascorbic acid and isoeugenol reacted quickly with the DPPH* reaching a steady state immediately. Rosmarinic acid and δ-tocopherol reacted a little slower and reached a steady state within 30 min. The remaining compounds reacted more progressively with the DPPH* reaching a steady state from 1 to 6 h. Caffeic acid, gentisic acid and gallic acid showed the highest antiradical activities with a stoichiometry of 4 to 6 reduced DPPH* molecules per molecule of antioxidant. Vanillin, phenol, γ-resorcylic acid and vanillic acid were found to be poor antiradical compounds. The stoichiometry for the other 13 phenolic compounds varied from one to three reduced DPPH* molecules per molecule of antioxidant. Possible mechanisms are proposed to explain the experimental results.

18,907 citations


"Comparison of ABTS, DPPH, FRAP, and..." refers background or methods in this paper

  • ... Brand-Williams et al. (1995) with some modifications....

    [...]

  • ...(Leong and Shui, 2002; Miller and Rice-Evans, 1997), 2,2diphenyl-1-picrylhydrazyl (DPPH) ( Brand-Williams et al., 1995; Gil et al., 2002), ferric reducing antioxidant power (FRAP) (Benzie and Strain, 1999; Guo et al., 2003; Jimenez-Escrig et al., 2001), and the oxygen radical absorption capacity (ORAC) (Cao et al., 1993; Ou et al., 2001; Prior et al., 2003)....

    [...]

  • ...…of Food Composition and Analysis 19 (2006) 669–675670 (Leong and Shui, 2002; Miller and Rice-Evans, 1997), 2,2- diphenyl-1-picrylhydrazyl (DPPH) (Brand-Williams et al., 1995; Gil et al., 2002), ferric reducing antioxidant power (FRAP) (Benzie and Strain, 1999; Guo et al., 2003; Jimenez-Escrig…...

    [...]

  • ...The DPPH assay was done according to the method of Brand-Williams et al. (1995) with some modifications....

    [...]

Journal ArticleDOI
TL;DR: The FRAP assay offers a putative index of antioxidant, or reducing, potential of biological fluids within the technological reach of every laboratory and researcher interested in oxidative stress and its effects.

17,394 citations


"Comparison of ABTS, DPPH, FRAP, and..." refers methods in this paper

  • ...The FRAP assay was done according to Benzie and Strain (1996) with some modifications....

    [...]

  • ...The DPPH assay was done according to the method of Brand-Williams et al. (1995) with some modifications....

    [...]

Journal ArticleDOI
TL;DR: In this paper, methods for quantitative analysis of anthocyanins, leuco-anthocyanin, flavanols, and total phenols in plant tissue extracts are described.
Abstract: Methods for the quantitative analysis of anthocyanins, leuco-anthocyanins, flavanols and total phenols in plant tissue extracts are critically examined and suitable modifications of existing methods are described.

3,501 citations


"Comparison of ABTS, DPPH, FRAP, and..." refers methods in this paper

  • ...Fruit extracts for total phenolics and antioxidant activity measured in methanol extract (AOAM) analysis were prepared using the method of Swain and Hillis (1959), with some modifications....

    [...]

  • ...The ascorbic acid concentration was calculated by comparison with the standard and expressed as mg/ 100 g fresh mass. Total phenolics content was determined by the Folin– Ciocalteu method, which was adapted from Swain and Hillis (1959)....

    [...]

  • ...Whole fruit was stored at 20 1C for 6 months before extraction....

    [...]

Book ChapterDOI
TL;DR: The ferric reducing/antioxidant power (FRAP) assay is a recently developed, direct test of “total antioxidant power” that facilitates experimental and clinical studies investigating the relationship among antioxidant status, dietary habits, and risk of disease.
Abstract: Publisher Summary This chapter discusses ferric reducing/antioxidant power (FRAP) assay. The ferric reducing/antioxidant power (FRAP) assay is a recently developed, direct test of “total antioxidant power.” The FRAP assay is robust, sensitive, simple, and speedy and facilitates experimental and clinical studies investigating the relationship among antioxidant status, dietary habits, and risk of disease. Measurement of the total antioxidant power of fresh biological fluids—such as blood plasma—can be measured directly; the antioxidant content of various dietary agents can be measured objectively and reproducibly and their potential for improving the antioxidant status of the body investigated and compared. The FRAP assay is also sensitive and analytically precise enough to be used in assessing the bioavailability of antioxidants in dietary agents to help monitor longitudinal changes in antioxidant status associated with an increased intake of dietary antioxidants and to investigate the effects of disease on antioxidant status.

3,037 citations


"Comparison of ABTS, DPPH, FRAP, and..." refers background in this paper

  • ..., 2002), ferric reducing antioxidant power (FRAP) (Benzie and Strain, 1999; Guo et al., 2003; Jimenez-Escrig et al., 2001), and the oxygen radical absorption capacity (ORAC) (Cao et al....

    [...]

  • ...…Rice-Evans, 1997), 2,2- diphenyl-1-picrylhydrazyl (DPPH) (Brand-Williams et al., 1995; Gil et al., 2002), ferric reducing antioxidant power (FRAP) (Benzie and Strain, 1999; Guo et al., 2003; Jimenez-Escrig et al., 2001), and the oxygen radical absorption capacity (ORAC) (Cao et al., 1993; Ou et…...

    [...]

Trending Questions (1)
When to use dpph,frap, ABTS and ORAC method FOR FRUIT JUICE?

DPPH, FRAP, ABTS, and ORAC methods can be used to measure antioxidant activity in fruit juice.