Comparison of approaches to quantify SARS-CoV-2 in wastewater using RT-qPCR: Results and implications from a collaborative inter-laboratory study in Canada
Alex H.S. Chik,Alex H.S. Chik,Melissa B. Glier,Mark R. Servos,Chand S. Mangat,Xiao-Li Pang,Yuanyuan Qiu,Patrick M. D'Aoust,Jean-Baptiste Burnet,Robert Delatolla,Sarah Dorner,Qiudi Geng,John P. Giesy,Robert Mike McKay,Michael R. Mulvey,Michael R. Mulvey,Natalie Prystajecky,Natalie Prystajecky,Nivetha Srikanthan,Yuwei Xie,Bernadette Conant,Steve E. Hrudey +21 more
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TLDR
In this paper, a real wastewater sample was spiked with surrogates of SARS-CoV-2 and human coronavirus strain 229E [HCoV]-strains at low and high levels then provided to eight laboratories.Abstract:
Detection of SARS-CoV-2 RNA in wastewater is a promising tool for informing public health decisions during the COVID-19 pandemic. However, approaches for its analysis by use of reverse transcription quantitative polymerase chain reaction (RT-qPCR) are still far from standardized globally. To characterize inter- and intra-laboratory variability among results when using various methods deployed across Canada, aliquots from a real wastewater sample were spiked with surrogates of SARS-CoV-2 (gamma-radiation inactivated SARS-CoV-2 and human coronavirus strain 229E [HCoV-229E]) at low and high levels then provided “blind” to eight laboratories. Concentration estimates reported by individual laboratories were consistently within a 1.0-log10 range for aliquots of the same spiked condition. All laboratories distinguished between low- and high-spikes for both surrogates. As expected, greater variability was observed in the results amongst laboratories than within individual laboratories, but SARS-CoV-2 RNA concentration estimates for each spiked condition remained mostly within 1.0-log10 ranges. The no-spike wastewater aliquots provided yielded non-detects or trace levels (read more
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Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance
Warish Ahmed,Stuart L. Simpson,Paul M. Bertsch,Kyle Bibby,Aaron Bivins,Linda L. Blackall,Sílvia Bofill-Mas,Albert Bosch,João Brandão,Phil M. Choi,Phil M. Choi,Mark Ciesielski,Erica Donner,Nishita D'Souza,Andreas H. Farnleitner,Andreas H. Farnleitner,Daniel Gerrity,Raul Gonzalez,John F. Griffith,Pradip Gyawali,Charles N. Haas,Kerry A. Hamilton,Hapuarachchige Chanditha Hapuarachchi,Valerie J. Harwood,Rehnuma Haque,Greg Jackson,Stuart J. Khan,Wesaal Khan,Masaaki Kitajima,Asja Korajkic,Giuseppina La Rosa,Blythe A. Layton,Erin K. Lipp,Sandra L. McLellan,Brian R. McMinn,Gertjan Medema,Suzanne Metcalfe,Wim G. Meijer,Jochen F. Mueller,Heather M. Murphy,Coleen C. Naughton,Rachel T. Noble,Sudhi Payyappat,Susan Petterson,Tarja Pitkänen,Tarja Pitkänen,Verónica Beatriz Rajal,Brandon Reyneke,Fernando A. Roman,Joan B. Rose,Marta Rusiñol,Michael J. Sadowsky,Laura Sala-Comorera,Yin Xiang Setoh,Samendra P. Sherchan,Kwanrawee Sirikanchana,Wendy Smith,Joshua A. Steele,Rosalie Subburg,Erin M. Symonds,Phong K. Thai,Kevin V. Thomas,Josh Tynan,Simon Toze,Janelle R. Thompson,Andrew S. Whiteley,Judith Chui Ching Wong,Daisuke Sano,Stefan Wuertz,Stefan Wuertz,Irene Xagoraraki,Qian Zhang,Amity G. Zimmer-Faust,Orin C. Shanks +73 more
TL;DR: A technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors.
Journal ArticleDOI
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance
TL;DR: In this paper , a technical review of factors that can cause false-positive and false-negative errors in the surveillance of SARS-CoV-2 RNA in wastewater, culminating in recommended strategies that can be implemented to identify and mitigate some of these errors.
Journal ArticleDOI
Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK.
Luke S. Hillary,Kata Farkas,Kathryn H. Maher,Anita Lucaci,Jamie Thorpe,Marco A. Distaso,William H. Gaze,Steve Paterson,Terry Burke,Thomas R. Connor,James E. McDonald,Shelagh K. Malham,David L. Jones +12 more
TL;DR: In this paper, the authors present a longitudinal analysis of SARS-CoV-2 RNA prevalence in sewage across six major urban centres in the UK (total population equivalent 3 million) by q(RT-)PCR and viral genome sequencing.
Journal ArticleDOI
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Xavier Fernandez-Cassi,Andreas Scheidegger,Carola Bänziger,Federica Cariti,Alex Tunas Corzon,Pravin Ganesanandamoorthy,Joseph C. Lemaitre,Christoph Ort,Timothy R. Julian,Timothy R. Julian,Timothy R. Julian,Tamar Kohn +11 more
TL;DR: In this article, the authors investigated how the dynamics of new COVID-19 infections estimated based on wastewater monitoring or confirmed cases compare to true COVID19 incidence dynamics, and they focused on the first pandemic wave in Switzerland (February to April, 2020), when test positivity ranged up to 26%.
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Social Media Efficacy in Crisis Management: Effectiveness of Non-pharmaceutical Interventions to Manage COVID-19 Challenges
TL;DR: This study suggests that intervention strategies can control the rapid spread of COVID-19 with hands-on crisis management measures, and the healthcare system will resume normal conditions quickly and the global economy will revitalize scientific contributions and collaborations through government support.
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