Comparison of Membrane Protein Glycopeptides of Sindbis Virus and Vesicular Stomatitis Virus
TL;DR: Preliminary evidence argues that the carbohydrate moiety covalently bound to different virus-specified membrane proteins may be specified principally by the host.
Abstract: A comparison has been made of the membrane glycoproteins and glycopeptides from two enveloped viruses, Sindbis virus and vesicular stomatitis virus (VSV). Glycopeptides isolated from Sindbis virus and VSV grown in the same host appear to differ principally in the number of sialic acid residues per glycopeptide; when sialic acid is removed by mild acid treatment, the glycopeptides of the two viral proteins are indistinguishable by exclusion chromatography. Preliminary evidence argues that the carbohydrate moiety covalently bound to different virus-specified membrane proteins may be specified principally by the host.
Citations
More filters
TL;DR: The results suggest that the lack of hemagglutinating activity of mutant virus grown at 39.5° is a consequence of the formation of aggregates of virus particles carrying neuraminic acid on their surface, and that the ts defect is in the neuraminidase but not the hemagGLutinin molecule.
810 citations
01 Jan 1975
TL;DR: Measles, canine distemper, and rinderpest viruses form a distinct subgroup on the basis of antigenicity, hemagglutinating characteristics, and lack of evidence for a virion-associated neuraminidase or neuraminic acid-containing cellular receptors, but it is now generally accepted that these viruses should be included in the paramyxovirus group because of their similar structural properties.
Abstract: The paramyxovirus group is a large one which includes the parainfluenza viruses types 1–5, Newcastle disease, and mumps viruses. Measles, canine distemper, and rinderpest viruses form a distinct subgroup on the basis of antigenicity, hemagglutinating characteristics, and lack of evidence for a virion-associated neuraminidase or neuraminic acid-containing cellular receptors. However, it is now generally accepted that these viruses should also be included in the paramyxovirus group because of their similar structural properties. Other more recently isolated viruses which have been classified as paramyxoviruses on the basis of morphological and biological properties are Yucaipa (Dinter et al., 1964) and Nariva (Walder, 1971) viruses. Table 1 lists paramyxoviruses and their primary hosts.
253 citations
01 Jan 1975
TL;DR: The rhabdoviruses are ubiquitous, highly infectious agents of animal and plant disease and are generally transmitted by arthropods and are, therefore, susceptible to disruption by ether and detergents.
Abstract: The rhabdoviruses are ubiquitous, highly infectious agents of animal and plant disease and are generally transmitted by arthropods. Assignment of viruses to the taxon rhabdoviruses (rod-shaped viruses) was originally based entirely on morphology. This classification has turned out to be fortuitously fortunate because later biochemical studies have revealed remarkable uniformity among these structurally similar viruses isolated from extremely diverse hosts. It is perhaps not farfetched to postulate a common ancestor for all the rhabdoviruses of plants, arthropods, and vertebrates. Classification of a virus as a rhabdovirus should be based on the following most important characteristics:
1.
Rhabdoviruses are rod-shaped particles, varying considerably in length (60–400 nm) but of a reasonably consistent width (60–85 nm).
2.
Animal rhabdoviruses tend to be bullet-shaped in appearance, flat at one end and a tapered sphere at the other. Plant rhabdoviruses are usually bacilliform in shape, quite elongated and with two round ends.
3.
All rhabdoviruses appear to be surrounded by a membranous envelope with protruding spikes. All these viruses probably contain lipids and are, therefore, susceptible to disruption by ether and detergents.
234 citations
TL;DR: The glycoprotein, but no other virion protein, of vesicular stomatitis virus was solubilized by the nonionic detergent Triton X-100 in low ionic strength buffer and induced the synthesis of antibody that formed a single precipitin line with the glycop protein and neutralized the infectivity of the virus.
Abstract: The glycoprotein, but no other virion protein, of vesicular stomatitis virus was solubilized by the nonionic detergent Triton X-100 in low ionic strength buffer. The solubilized viral glycoprotein induced the synthesis of antibody that formed a single precipitin line with the glycoprotein and that neutralized the infectivity of the virus. The neutralizing activity of the antibody was efficiently blocked by purified glycoprotein.
223 citations
TL;DR: The finding of multiple pieces of messenger RNA, all smaller than 40 S virion RNA, suggests that VSV polypeptides are synthesized from individual molecules of RNA, rather than from one large messenger RNA.
209 citations
References
More filters
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
19,381 citations
"Comparison of Membrane Protein Glyc..." refers background in this paper
..., in press) and compare favorably with other molecular-weight standards which have been extensively calibrated by acrylamide gel electrophoresis (26)....
[...]
3,602 citations
"Comparison of Membrane Protein Glyc..." refers background in this paper
...(12), the four VSV polypeptides have estimated molecular weights of 300,000, 73,000, 49,000, and 25,000....
[...]
1,382 citations
TL;DR: This chapter discusses the procedures employed for the analysis of the sugar components of glycoproteins, and the hydrolytic release of the monosaccharides is also considered.
Abstract: Publisher Summary This chapter discusses the procedures employed for the analysis of the sugar components of glycoproteins. Most of the methods used for the estimation of the monosaccharides present in glycoproteins are colorimetric in nature. Because of the relatively low specificity of some of these color reactions, it is often important to apply them only after appropriate separation of the constituents. In many cases complete identification, preferably by chromatographic means, of all the sugar and amino acid constituents of a glycoprotein should precede any colorimetric analysis so that potentially interfering substances may be taken into account. The hydrolytic release of the monosaccharides is also considered in the chapter. The optimal conditions of acid hydrolysis for the monosaccharides vary substantially because of differences both in the stability of their glycosidic bonds and in their susceptibility to destruction during the hydrolysis. One of the prerequisites for an accurate analysis of the monosaccharide components of a polymer is finding hydrolysis conditions under which all of a given sugar is released and yet is not significantly destroyed.
1,231 citations
"Comparison of Membrane Protein Glyc..." refers background or methods in this paper
...Sucrose and fetuin glycopeptides were used as additional markers and were detected by the anthrone reaction, and sialic acid by the Warren thiobarbituric acid assay as described by Spiro (16)....
[...]
...which is sufficient to remove terminally bound sialic acid residues (16)....
[...]
544 citations