Journal ArticleDOI
Comparison of the SpermMar test with currently accepted procedures for detecting human sperm antibodies.
D.J. Kay,B Boettcher +1 more
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TLDR
The SpermMar test proved easier to use and to assess than the Immunobead test and it is recommended for consideration as a screening procedure for sperm antibodies despite the fact that at this stage only IgG antibodies can be detected.Abstract:
To eliminate the possibility of immunological infertility in spontaneously infertile and re-anastomosed men, a screening test that can be applied directly to semen is desirable. The SpermMar test is one such possibility. In this study, indirect tests for sperm antibodies using the commercial SpermMar test have been applied to a panel of sera whose reactions in the tube slide agglutination test (TSAT), gelatin agglutination test (GAT) and sperm immobilization test (SIT) for sperm antibodies are well characterized. The results from the SpermMar tests are compared directly with those obtained from Immunobead tests carried out at the same time. Results from screening tests performed on 30 sera confirmed complete correspondence between the GAT, SpermMar and Immunobead tests. When sera were titrated, the Immunobead test proved slightly more sensitive than the GAT and the SpermMar test was slightly more sensitive than the Immunobead test. The SpermMar test proved easier to use and to assess than the Immunobead test and it is recommended for consideration as a screening procedure for sperm antibodies despite the fact that at this stage only IgG antibodies can be detected.read more
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Journal ArticleDOI
Infertility due to antisperm antibodies
TL;DR: In approaching couples with infertility, a high index of suspicion for antibodies is necessary to avoid misdiagnosis, and in the optimal situation, all semen analyses should be screened for sperm-bound antibodies, but if this is impractical, testing should be performed on high-risk individuals.
Journal ArticleDOI
Varicocele and antisperm antibody: fact or fiction?
TL;DR: Varicocelectomy may reduce the ASAB level, and this reduction has a good effect on semen parameter quality, and the level of antisperm antibodies (ASAB) in the serum and semen of infertile men with varicocele is raised in some patients.
Journal ArticleDOI
Antisperm antibodies: Use of the mixed agglutination reaction (MAR) test using latex beads
A Mahmoud,Frank Comhaire +1 more
TL;DR: The purpose of a debate contribution is to stir up controversy and discussion and a recent paper has done exactly that but aside from several criticisms already highlighted it seems that the view-point of Helmerhorst et al. (1999) is biased among other things by the selection of references.
Journal ArticleDOI
Antisperm antibodies detected by mixed agglutination reaction and immunobead test are not associated with chronic inflammation and infection of the seminal tract.
TL;DR: The MAR test and immunobead test have a statistical significant correlation and their results provide evidence that there is no association between inflammatory/infectious diseases of the male reproductive tract and the presence of ASA in semen.
Journal ArticleDOI
A comparison of the sperm mixed-agglutination reaction test with the peroxidase-labelled protein A test for detecting antisperm antibodies in infertile men with varicocele
TL;DR: To compare the sperm mixed‐agglutination reaction (sMAR) with the peroxidase‐labelled protein A method (POPA) in infertile patients with varicocele, the objective was to establish an experimental procedure and show direct comparison between the two methods.
References
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Journal ArticleDOI
Immunologic analysis of sperm-immobilizing factor found in sera of women with unexplained sterility
TL;DR: It was demonstrated that the sperm immobilization test was the best procedure and the sperm-immobilizing factor in patient's serum which gave the strongest positive test was analyzed immunologically.
Journal ArticleDOI
Methods for the detection of antibodies against mammalian spermatozoa. II. A gelatin agglutination test.
TL;DR: An improved macroscopic test for the detection of agglutinins against human spermatozoa and a medium of increased viscosity containing 2.5% gelatin is described.
Journal ArticleDOI
Antispermatozoal antibody and unexplained infertility.
Robert R. Franklin,C. Dean Dukes +1 more
Journal Article
A simple method of screening for antisperm antibodies in the human male. Detection of spermatozoal surface IgG with the direct mixed antiglobulin reaction carried out on untreated fresh human semen.
TL;DR: The percentage of motile spermatozoa found to be coated with antisperm antibody of the IgG class, and the extent of the coating, proved to be correlated with the agglutination titer of circulating antisperm antibodies and with the inhibition of sperm penetration into cervical mucus.
Journal Article
Techniques for detection of iso- and auto-antibodies to human spermatozoa.
TL;DR: Techniques for the detection of iso- and autoantibodies to human spermatozoa and potential application of immunologic techniques in the control of fertility and general investigative methods are discussed.