Journal ArticleDOI
Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging
Pietro Ferraro,Sergio De Nicola,Andrea Finizio,Giuseppe Coppola,Simonetta Grilli,Carlo Magro,Giovanni Pierattini +6 more
Reads0
Chats0
TLDR
An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes and it is shown that a correction effect can be obtained at all reconstruction planes.Abstract:
An approach is proposed for removing the wave front curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optical parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures.read more
Citations
More filters
Journal ArticleDOI
Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip
TL;DR: This work investigates the problem of counting and estimating velocity of cells flowing inside a scattering chip and proposes and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect.
Journal ArticleDOI
Optical-mechanical signatures of cancer cells based on fluctuation profiles measured by interferometry.
TL;DR: This study quantitatively measured the time‐dependent, nanometer‐scale optical thickness fluctuation maps of live cells in vitro and found that cancer cells fluctuate significantly more than healthy cells, and that metastatic cancer Cells fluctuateificantly more than primary cancer cells.
Journal ArticleDOI
Boundary-artifact-free phase retrieval with the transport of intensity equation II: applications to microlens characterization.
TL;DR: Experimental results on microlenses highlight the importance of boundary conditions that often overlooked in simplified models, and confirm that the new boundary-artifact-free TIE phase retrieval method effectively avoid the boundary error even when objects are located at the image borders.
Journal ArticleDOI
Surface topography of microstructures in lithium niobate by digital holographic microscopy
S. De Nicola,Pietro Ferraro,Andrea Finizio,Simonetta Grilli,Giuseppe Coppola,Mario Iodice,P. De Natale,M. Chiarini +7 more
TL;DR: In this article, the application of holographic microscopy as a metrological tool for inspection and the micro-topography reconstruction of different microstructures fabricated in bulk lithium niobate by differential etching of reversed ferroelectric domain patterned crystals is reported.
Journal ArticleDOI
Microlens characterization by digital holographic microscopy with physical spherical phase compensation
TL;DR: There is one, and only one, reference fiber point position to give a reference wavefront that is quasi-identical to the object wavefront where the system is free of wavefront aberration and directly gives the quantitative phase of the test object without the need for complicated numerical compensation.
References
More filters
Journal ArticleDOI
Phase-shifting digital holography
Ichirou Yamaguchi,Tong Zhang +1 more
TL;DR: A new method is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer, which can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration.
Journal ArticleDOI
Direct recording of holograms by a CCD target and numerical reconstruction.
Ulf Schnars,W. Jüptner +1 more
TL;DR: The principle of recording holograms directly on a CCD target is described and a real image of the object is reconstructed from the digitally sampled hologram by means of numerical methods.
Journal ArticleDOI
Digital holography for quantitative phase-contrast imaging.
TL;DR: A new application of digital holography for phase-contrast imaging and optical metrology and an application to surface profilometry shows excellent agreement with contact-stylus probe measurements.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
Journal ArticleDOI
Digital recording and numerical reconstruction of holograms
Ulf Schnars,Werner Jüptner +1 more
TL;DR: The principles and major applications of digital recording and numerical reconstruction of holograms (digital holography) are described, which are applied to measure shape and surface deformation of opaque bodies and refractive index fields within transparent media.
Related Papers (5)
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
Digital holography for quantitative phase-contrast imaging.
Direct recording of holograms by a CCD target and numerical reconstruction.
Ulf Schnars,W. Jüptner +1 more