Journal ArticleDOI
Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging
Pietro Ferraro,Sergio De Nicola,Andrea Finizio,Giuseppe Coppola,Simonetta Grilli,Carlo Magro,Giovanni Pierattini +6 more
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TLDR
An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes and it is shown that a correction effect can be obtained at all reconstruction planes.Abstract:
An approach is proposed for removing the wave front curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optical parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures.read more
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Journal ArticleDOI
Partially coherent lensfree tomographic microscopy [Invited]
TL;DR: In this article, in-line holograms of objects at different angles of partially coherent illumination are recorded using a digital sensor-array, which enables computing pixel super-resolved tomographic images of the specimen.
Journal ArticleDOI
Green's formulation for robust phase unwrapping in digital holography
L. Aiello,Daniele Riccio,Pietro Ferraro,Simonetta Grilli,Lucia Sansone,Giuseppe Coppola,S. De Nicola,Andrea Finizio +7 more
TL;DR: In this paper, a phase unwrapping method based on an algorithm which makes use of Green's first identity was presented, which aims at recovering the correct phase information encoded in a fringe pattern after digital holography (DH) numerical processing.
Journal ArticleDOI
High speed moire based phase retrieval method for quantitative phase imaging of thin objects without phase unwrapping or aberration compensation
Shouyu Wang,Keding Yan,Liang Xue +2 more
TL;DR: A high speed moire based phase retrieval method is proposed which is capable of calculating quantitative phase distributions without phase unwrapping or aberration compensation and it is believed that the proposed method will be useful in real time phase observations and measurements.
Journal ArticleDOI
Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy.
TL;DR: A novel algorithm for sequential processing of living cells phase images in a time‐lapse sequence that compensates for the deformation of a phase image using weighted least‐squares surface fitting and identifies and segments the individual cells in the phase image.
Journal ArticleDOI
Two-channel algorithm for single-shot, high-resolution measurement of optical wavefronts using two image sensors.
TL;DR: A two-channel algorithm optimized for the 2ch-HDI to compensate for differences in the intensity distributions in the two image sensors is developed and results show that this algorithm significantly enhances measurement accuracy.
References
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Journal ArticleDOI
Phase-shifting digital holography
Ichirou Yamaguchi,Tong Zhang +1 more
TL;DR: A new method is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer, which can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration.
Journal ArticleDOI
Direct recording of holograms by a CCD target and numerical reconstruction.
Ulf Schnars,W. Jüptner +1 more
TL;DR: The principle of recording holograms directly on a CCD target is described and a real image of the object is reconstructed from the digitally sampled hologram by means of numerical methods.
Journal ArticleDOI
Digital holography for quantitative phase-contrast imaging.
TL;DR: A new application of digital holography for phase-contrast imaging and optical metrology and an application to surface profilometry shows excellent agreement with contact-stylus probe measurements.
Journal ArticleDOI
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
TL;DR: Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation to show that the transverse resolution is equal to the diffraction limit of the imaging system.
Journal ArticleDOI
Digital recording and numerical reconstruction of holograms
Ulf Schnars,Werner Jüptner +1 more
TL;DR: The principles and major applications of digital recording and numerical reconstruction of holograms (digital holography) are described, which are applied to measure shape and surface deformation of opaque bodies and refractive index fields within transparent media.
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Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms
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