scispace - formally typeset
Search or ask a question
Journal ArticleDOI

Complementarity between Rous sarcoma virus (RSV) RNA and the in vitro-synthesized DNA of the virus-associated DNA polymerase.

01 Nov 1970-Virology (Academic Press)-Vol. 42, Iss: 3, pp 783-788
About: This article is published in Virology.The article was published on 1970-11-01. It has received 96 citations till now. The article focuses on the topics: Hepatitis B virus DNA polymerase & RNA-dependent RNA polymerase.
Citations
More filters
Book ChapterDOI
TL;DR: It is hypothesized that the leukovirus RNA-directed DNA polymerase activity is an integral part of the ribonucleoprotein core of the virions, which suggests that the virion enzyme activity is related to normal cellular DNA polymerases, and that there are homologies between the amino acid sequences of the viral enzyme and normal cellular enzymes.
Abstract: Publisher Summary The discovery of RNA-directed DNA synthesis in disrupted virions of RNA tumor viruses added strong support to the hypothesis that information transfer from RNA to DNA exists in biological system. The chapter discusses the properties of the endogenous reaction carried out by the virion DNA polymerase. To study the endogenous reaction disrupted, virions are incubated with substrates in the absence of any added template and synthesis of DNA is observed using the RNA present in the virions as template. The chapter also discusses the general implications of RNA-directed DNA synthesis in relation to tumor viruses, neoplastic cells, and normal cells. It is hypothesized that the leukovirus RNA-directed DNA polymerase activity is an integral part of the ribonucleoprotein core of the virions. The cores are synthesized in cells as precursor particles and then are incorporated into complete virions when the virions are assembled by budding at the cell surface. This core enzyme system contains not only the template-primer RNA, a DNA polymerase that can transfer information from RNA to double-stranded DNA, but ancillary enzymes, such as polynucleotide ligase and nucleases, which may aid in integrating the viral information with cellular DNA. This suggests that the virion enzyme activity is related to normal cellular DNA polymerases, and that there are homologies between the amino acid sequences of the viral enzyme and normal cellular enzymes. The relationship of RNA-directed DNA synthesis to neoplasia depends upon the relationship of RNA tumor viruses to neoplasia, which is supported by three general hypotheses: the provirus model, the oncogene model, and the protovirus model.

400 citations

Journal ArticleDOI
TL;DR: DNA polymerase from avian myeloblastosis virus has been purified by a combination of column chromatography and gel filtration methods and possesses the RNA-, DNA-, and hybrid-directed polymerase activities found in the virion.

276 citations

Journal ArticleDOI
01 Mar 1974-Nature
TL;DR: Nucleic acid hybridisation with a 3H-DNA transcript of the viral genome demonstrates that this virus is genetically different from previously studied mammalian C-type viruses, and suggests that M7 is an endogenous baboon virus.
Abstract: A C-type virus (“M7”) has been isolated from a specimen of baboon placenta cocultivated with various mammalian host cell lines. Nucleic acid hybridisation with a 3H-DNA transcript of the viral genome demonstrates that this virus is genetically different from previously studied mammalian C-type viruses, and suggests that M7 is an endogenous baboon virus.

253 citations

Journal ArticleDOI
TL;DR: Virus-specific RNA was found to exist in infected cells in three major size classes: 60–70 S RNA, 35 S RNA and 20–30 S RNA; and Messenger RNA was differentiated from other virus-specific RNAs by the criterion that virus- specific messenger RNA must change in sedimentation rate following polyribosome disaggregation.

213 citations

Journal ArticleDOI
09 Feb 1972-Nature
TL;DR: The following two articles describe successful syntheses in vitro of DNA copies of various mammalian globin mRNAs, using reverse transcriptase from avian myoblastosis virus, using poly(dT) sequences at their 3′-ends.
Abstract: The following two articles describe successful syntheses in vitro of DNA copies of various mammalian globin mRNAs, using reverse transcriptase from avian myoblastosis virus. Short stretches of poly(dT) greatly enhance this effect, presumably because they prime correct initiation of reverse transcription of these mRNAs by pairing with known poly (A) sequences at their 3′-ends.

202 citations

References
More filters
Journal ArticleDOI
27 Jun 1970-Nature
TL;DR: Two independent groups of investigators have found evidence of an enzyme in virions of RNA tumour viruses which synthesizes DNA from an RNA template, apparently the classical process of information transfer from DNA to RNA can be inverted.
Abstract: Two independent groups of investigators have found evidence of an enzyme in virions of RNA tumour viruses which synthesizes DNA from an RNA template. This discovery, if upheld, will have important implications not only for carcinogenesis by RNA viruses but also for the general understanding of genetic transcription: apparently the classical process of information transfer from DNA to RNA can be inverted.

1,872 citations

Journal ArticleDOI
27 Jun 1970-Nature
TL;DR: Viral RNA-dependent DNA Polymerase: RNA- dependent DNA polymerase in Virions of Rous Sarcoma Virus and its role in cell reprograming is studied.
Abstract: Viral RNA-dependent DNA Polymerase: RNA-dependent DNA Polymerase in Virions of Rous Sarcoma Virus

1,833 citations

Journal ArticleDOI
TL;DR: The rate of formation of complexes between T2 RNA and T2 DNA has been measured at various salt concentrations and temperatures and the resistance to RNase increased with increasing salt concentration but was not complete under any condition tested.

290 citations

Journal ArticleDOI
08 Aug 1970-Nature
TL;DR: Several RNA tumour viruses contain an enzyme that synthesizes a DNA–RNA hybrid using the single stranded viral RNA as template, and hybridization experiments confirm that the DNA strand is complementary to the viral RNA.
Abstract: Several RNA tumour viruses contain an enzyme that synthesizes a DNA–RNA hybrid using the single stranded viral RNA as template. Hybridization experiments confirm that the DNA strand is complementary to the viral RNA.

261 citations