Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea.
TL;DR: Phylogenetic analysis based on the RdRp amino acid sequences showed that BdOLV1 is grouped with oomycete-infecting unclassified viruses closely related to the genus Botoulivirus in Botourmiaviridae.
Abstract: Here, we describe the full-length genome sequence of a novel ourmia-like mycovirus, tentatively named "Botryosphaeria dothidea ourmia-like virus 1" (BdOLV1), isolated from the phytopathogenic fungus Botryosphaeria dothidea strain 8A, associated with apple ring rot in Shanxi province, China. The complete BdOLV1 genome is comprised of a 2797-nucleotide positive-sense (+) single-stranded RNA (ssRNA) with a single open reading frame (ORF). The ORF putatively encodes a 642-amino-acid polypeptide with conserved RNA-dependent RNA polymerase (RdRp) motifs related to those of viruses of the family Botourmiaviridae. Phylogenetic analysis based on RdRp amino acid sequences showed that BdOLV1 is grouped with unclassified oomycete-infecting viruses closely related to members of the genus Botoulivirus in the family Botourmiaviridae. This is the first report of a novel (+)ssRNA virus in B. dothidea related to members of the genus Botoulivirus in the family Botourmiaviridae.
Summary (1 min read)
- Botryosphaeria dothidea is a notorious canker pathogen that infects a wide range of trees worldwide  .
- This fungus is the principal causal agent of apple ring rot in China and is distributed across almost every apple planting region  .
- Several other mycoviruses have since been experimentally proven capable of introducing hypovirulence into their host fungi  .
- The partial viral sequence and all terminal sequences were assembled and analyzed using DNAMAN version 9.0 (Lynnon Biosoft).
- The complete genome of BdOLV1 was submitted to GenBank under accession no.
- The tree topology showed that BdOLV1 grouped (100% bootstrap support) with previously reported ourmia-like viruses from the oomycete Plasmopara viticola and ascomycete fungi B. dothidea and Phaeoacremonium minimum (Fig. 2 ).
- At present, it is phylogenetically di cult to conclude whether BdOLV1 and its closely related viruses are novel species within the genus Botoulivirus or whether they constitute a new genus in the family Botourmiaviridae.
- Highly and less conserved amino acid positions are indicated by black shading and white lettering, and gray shading and black lettering, respectively.
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Q1. What contributions have the authors mentioned in the paper "Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus" ?
Liying Sun et al. this paper proposed the Northwest Agriculture and Forestry University 's College of Plant Protection.