Complexity of begomovirus and betasatellite populations associated with chilli leaf curl disease in India.
Citations
95 citations
Cites background or result from "Complexity of begomovirus and betas..."
...In 1995, the association of ToLCNDV with tomato leaf curl disease on solanaceous crops was first reported in India [4]....
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...The earliest genetic characterization of ToLCNDV corresponds to isolates collected in India and Pakistan during 1990s (e.g., GenBank accession numbers U15015 and Y16421, or AJ620187, resp ctively, for their DNA-A components)....
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...Initial observation of the natural co-existence of ToLCNDV DNA-A with DNA-B component on tomatoes was done in India....
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...The evolved recombinant strain ToLCNDV-ES is adapted to infect cucurbits (zucchini squash, cucumber, melon) with poor adaptation for infecting tomato, unlike ToLCNDV isolates reported in India [7]....
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...Variants of ToLCNDV The earliest genetic characterization of ToLCNDV corresponds to isolates collected in India and Pakistan during 1990s (e.g., GenBank accession numbers U15015 and Y16421, or AJ620187, respectively, for their DNA-A components)....
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72 citations
Cites background from "Complexity of begomovirus and betas..."
...Studies in the last few decades revealed the association of begomoviruses with molecules like betasatellite, alphasatellite and deltasatellite (Kumar et al., 2015; Mansoor et al., 2006; Nawaz-ul-Rehman and Fauquet, 2009; Saunders et al., 2000; Vinoth Kumar et al., 2017)....
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...Betasatellites associated with chilli leaf curl disease in India, for instance, Tomato leaf curl Bangladesh betasatellite, contain high nucleotide variability and a high nucleotide substitution rate in the βC1 coding regions (Kumar et al., 2015)....
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...Betasatellites are widespread amongst the ‘Old World’ begomoviruses that are mostly monopartite in nature (Kumar et al., 2015; Saeed et al., 2007; Zubair et al., 2017)....
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...Amongst chilli-infecting betasatellites, the A-rich region and SCR have been reported as hot spots for recombination (Kumar et al., 2015)....
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48 citations
46 citations
Cites background or methods from "Complexity of begomovirus and betas..."
...Several begomoviruses are known to infect chilli and cause huge economic losses in India (Kumar et al. 2015)....
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...…Number of plants showing symptoms/number of plants inoculated b Severity of symptoms was scored from mild (+) to severe (++++++) according to Chattopadhyay et al. (2008) and Kumar et al. (2015) d Time taken for the first symptom appearance e Plants indicated presence of ChNDA and Toβ by PCR…...
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...Begomoviruses are often associated with betasatellites (Briddon et al. 2003; Chattopadhyay et al. 2008; Kumar et al. 2015)....
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...Virus inoculation of chilli plants was carried out by particle bombardment method (Kumar et al. 2015)....
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44 citations
References
39,110 citations
"Complexity of begomovirus and betas..." refers background or methods in this paper
...NEXUS files of AV1 and bC1 regions were generated using CLUSTAL W in MEGA5, and nucleotide substitution rate was estimated using the GTR nucleotide substitution model, uncorrelated lognormal relaxed clock model and BSP coalescence model in the BEAST v. 1.7 package (Drummond et al., 2012; Nawaz-ul-Rehman et al., 2012)....
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...MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods....
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..., 2014) and MEGA5 (Tamura et al., 2011), neighbour-joining phylogenetic dendrograms and percentage pairwise identity of the identified sequences and the representative sequences from the database were generated....
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...Using the CLUSTAL W method in SDT v. 1.0 (Muhire et al., 2014) and MEGA5 (Tamura et al., 2011), neighbour-joining phylogenetic dendrograms and percentage pairwise identity of the identified sequences and the representative sequences from the database were generated....
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27,454 citations
9,055 citations
"Complexity of begomovirus and betas..." refers methods in this paper
...…bC1 regions were generated using CLUSTAL W in MEGA5, and nucleotide substitution rate was estimated using the GTR nucleotide substitution model, uncorrelated lognormal relaxed clock model and BSP coalescence model in the BEAST v. 1.7 package (Drummond et al., 2012; Nawaz-ul-Rehman et al., 2012)....
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7,263 citations
"Complexity of begomovirus and betas..." refers methods in this paper
...Total genomic DNA was extracted from all samples (Dellaporta et al., 1983) and subjected to RCA using Q29 DNA polymerase (InoueNagata et al., 2004)....
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...Total genomic DNA was extracted from all samples (Dellaporta et al., 1983) and subjected to RCA using Q29 DNA polymerase (InoueNagata et al....
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6,100 citations
"Complexity of begomovirus and betas..." refers background or result in this paper
...Begomoviruses have been reported to be associated with ssDNA satellite molecules and/or satellite-like molecules, namely betasatellites and alphasatellites, respectively (Mansoor et al., 1999; Nawaz-ul-Rehman & Fauquet, 2009; Saunders et al., 2000)....
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...Furthermore, their dependence on betasatellites for severe disease development supports the notion that these monopartite begomoviruses require betasatellites for ChiLCD, similar to the complexes reported with ageratum yellow vein and cotton leaf curl diseases (Mansoor et al., 2006; Saunders et al., 2000)....
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...This observation is in agreement with earlier reports suggesting that the betasatellites are dispensable for disease development in N. benthamiana (Ranjan et al., 2014; Saunders et al., 2000)....
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...…their dependence on betasatellites for severe disease development supports the notion that these monopartite begomoviruses require betasatellites for ChiLCD, similar to the complexes reported with ageratum yellow vein and cotton leaf curl diseases (Mansoor et al., 2006; Saunders et al., 2000)....
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