Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
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Cites background from "Comprehensive Identification of Cel..."
...Another use for GO ontologies that is gaining rapid adherence is the annotation of gene-expression data, especially after these have been clustered by similarities in pattern of gene expressio...
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...Spellman et al. clustered 800 genes according to their expression during the yeast cell cycle [ 260 ]....
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...Generally, cluster analysis of gene expression data is composed of two aspects: clustering genes [80], [206], [ 260 ], [268], [283], [288] or clustering tissues or experiments [5], [109], [238]....
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5,165 citations
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Cites methods from "Comprehensive Identification of Cel..."
...This dataset recorded gene expression levels during different stages of cell cycles in yeasts and has been widely used before to illustrate clustering methods (Spellman et al., 1998)....
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References
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"Comprehensive Identification of Cel..." refers background or methods in this paper
...Mixtures of labeled control and experimental cDNAs were competitively hybridized to individual microarrays containing essentially all yeast genes (DeRisi et al., 1997)....
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...DNAs were prepared and printed onto microarrays as described previously (Shalon et al., 1996; DeRisi et al., 1997 [http:/ /cmgm....
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...DNAs were prepared and printed onto microarrays as described previously (Shalon et al., 1996; DeRisi et al., 1997 [http://cmgm. stanford.edu/pbrown/]; Eisen and Brown, 1999) with 190-mm spacing between the centers of each element....
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3,547 citations
"Comprehensive Identification of Cel..." refers background in this paper
...*Department of Genetics, Stanford University Medical Center, Stanford, California 94306-5120; ‡Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724-2209; §Department of Biochemistry, Stanford University Medical Center, Stanford, California 94306-5428; and \Howard Hughes Medical…...
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2,232 citations
"Comprehensive Identification of Cel..." refers background or methods in this paper
...Molecular Biology of the Cell Vol. 9, 3273–3297, December 1998 Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by...
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...The RNA was reverse transcribed using TAQman reverse transcription reagents (PE Applied Biosystems, Foster City, CA) consisting of 2.5 mM oligo-dT 16 mer, 1.25 U/ml MultiScribe reverse transcriptase, 0.5 mM dGTP, dATP, dTTP, and dCTP, 0.4 U/ml RNase inhibitor, 50 mM KCl, 10 mM Tris-HCl (pH 8.3)....
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...The microarrays were allowed to hybridize 4–6 h. Microarrays were removed from the chambers and placed in standard histochemistry slide holders where they were washed by plunging 30 times in each of the following solutions, respectively: 23 SSC, 0.2% SDS; 0.43 SSC; and 0.23 SSC....
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