Journal ArticleDOI
Computerized semen analysis (CASA): effect of semen concentration and chamber depth on measurements.
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TLDR
The results indicate that a real-time analysis system can be used clinically for semen analysis over a wide range of sperm concentration, however, high sperm concentration can distort sperm count, motility, and sperm kinematics recordings.Abstract:
The purpose of this study was to examine the influence of sample concentration and chamber depth on the performance of a real-time analysis computer-assisted semen analysis system, the Hobson Sperm Tracker. Fresh semen samples were provided by patients or donors who attended the author's clinic. The samples were used to estimate total concentration, percentage motility, and sperm kinematics. A considerable variation was found in total concentration and motility recordings between manual and computerized analysis, which was more profound in high-density samples (>80 x 10 6 sperm/mL). The sperm motion parameters were significantly different between low- and high-density samples. This difference could not have been due to sample variation since it was also observed after 1:10 dilution of dense samples. The results indicate that a real-time analysis system can be used clinically for semen analysis over a wide range of sperm concentration. However, high sperm concentration can distort sperm count, motility, and sperm kinematics recordings.read more
Citations
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Journal ArticleDOI
Computer‐aided sperm analysis: past, present and future
TL;DR: This review focuses on the concept of CASA, the development course of CAS a technology, the clinical application of CASa systems and the factors influencing the accuracies of results, such as frame rate, sperm counting chambers affiliated to the CASA system, algorithms and sperm concentration.
Journal Article
Comparison of different evaluation chambers for analysis of rabbit spermatozoa motility parameters using CASA system
Peter Massanyi,Peter Chrenek,Norbert Lukáč,Alexander V. Makarevich,A. Ostro,J. Živčák,Jozef Bulla +6 more
TL;DR: In this paper, the authors compared spermatozoa motility parameters, measured using different evaluation chambers, were compared and found significant differences in almost all studied parameters in regards to evaluation chamber used.
Journal ArticleDOI
In vitro effect of nickel on bovine spermatozoa motility and annexin V-labeled membrane changes.
Norbert Lukáč,Laszlo Bardos,Robert Stawarz,Shubhadeep Roychoudhury,Alexander V. Makarevich,Peter Chrenek,Jan Danko,Peter Massanyi +7 more
TL;DR: In the group with the highest concentration and the longest time of exposure the apoptotic Annexin‐positive regions were detected not only in the mitochondrial part, but also in the spermatozoa head (acrosomal and postACrosomal part), showing significant alteration of spermatozosa membrane integrity.
Journal ArticleDOI
Semen analysis standardization: is there any problem in Polish laboratories?
Renata Walczak-Jędrzejowska,Katarzyna Marchlewska,Elżbieta Oszukowska,Eliza Filipiak,Leszek Bergier,Jolanta Słowikowska-Hilczer +5 more
TL;DR: There is a need for standardisation of the methods and continuous, unified training in semen analysis in Polish laboratories, with regard to semen analysis methodology.
Journal ArticleDOI
Effect of chamber characteristics, loading and analysis time on motility and kinetic variables analysed with the CASA-mot system in goat sperm
TL;DR: There were no significant differences in total sperm motility between different chamber depths, however, use of the 20μm-chambers resulted in greater sperm progressive motility rate, VSL and LIN, and less VCL and VAP than chambers with a lesser depth.
References
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Journal ArticleDOI
Statistical methods for assessing agreement between two methods of clinical measurement.
TL;DR: An alternative approach, based on graphical techniques and simple calculations, is described, together with the relation between this analysis and the assessment of repeatability.
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