Fig. 1. Immediate-early (IE) gene transcripts of IIV6. Cells were infected with IIV6 in the presence of DNA (Ara-C) or protein synthesis (CHX) inhibitors. ORF-specific RT-PCR was carried out on total RNA isolated at 12 h post infection. Genes that give a positive RT-PCR signal in the presence of these inhibitors are categorized as IE genes. ORF: open reading frame; Ara-C: DNA synthesis inhibitor (cytosine arabinoside); CHX: protein synthesis inhibitor (cycloheximide). PC: Positive control, infection without addition of inhibitors.
...read more