Fig. 2. Delayed-early (DE) and late (L) gene transcripts of IIV6. Cells were infected with IIV6 in the presence of DNA (Ara-C) or protein synthesis (CHX) inhibitors. ORF-specific RT-PCR was carried out on total RNA isolated at 12 h post infection. Transcripts that were not detected in the presence of the protein synthesis inhibitor (CHX) but were not affected by the DNA synthesis inhibitor are from DE genes (A). Genes that do not show transcripts in the presence of both inhibitors as categorized as L genes (B). ORF: open reading frame; Ara-C: DNA synthesis inhibitor (cytosine arabinoside); CHX: protein synthesis inhibitor (cycloheximide). PC: Positive control, infection without addition of inhibitors.
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