Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
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The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.Abstract:
THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out.read more
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References
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Journal ArticleDOI
Plaque Formation in Agar by Single Antibody-Producing Cells
Niels K. Jerne,Albert A. Nordin +1 more
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
Journal ArticleDOI
Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants
TL;DR: Evidence can be obtained which suggests that mating may be followed by segregation in mice, and when two clonal lines of mouse fibroblasts are grown together for 4 days, hybrid cells can be detected by selective conditions.
Journal ArticleDOI
Progress in immunology: Syndromes of diminished resistance to infection
TL;DR: In this review of progress in immunology, an attempt is made to synthesize some of the rapidly accumulating observations in clinical medicine and experimental biology into a workable scheme, which may help pediatricians in their approach to the study and management of patients suspected of abnormalities in their resistance to infection.
Journal ArticleDOI
Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species.
Henry Harris,J. F. Watkins +1 more
TL;DR: Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different Species is presented, which describes how cells from different species can be hybrids.
Journal ArticleDOI
Mouse myelomas and lymphomas in culture
K. Horibata,A.W. Harris +1 more
TL;DR: Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells, suggesting that in these respects the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells.