Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
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TLDR
The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.Abstract:
THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out.read more
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Making Antibodies by Phage Display Technology
TL;DR: Human antibody fragments with many different binding specificities have been isolated from the same phage repertoire, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus.
Journal ArticleDOI
AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
Amy Yin,Sheri Miraglia,Esmail D. Zanjani,Graça Almeida-Porada,Makio Ogawa,Anne G. Leary,Johanna Olweus,John F. Kearney,David William Buck +8 more
TL;DR: AC133-selected cells engraft successfully in a fetal sheep transplantation model, and human cells harvested from chimeric fetal sheep bone marrow have been shown to successfully engraft secondary recipients, providing evidence for the long-term repopulating potential of AC133(+) cells.
Journal ArticleDOI
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
TL;DR: This paper used hybridoma monoclonal antibodies obtained after immunization of mice with rat cells to study rat cell-surface antigens present on subpopulations of rat lymphocytes.
Journal ArticleDOI
Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95
TL;DR: The yeast two-hybrid system was used to show that the cytoplasmic tails of NMDA receptor subunits interact with a prominent postsynaptic density protein PSD-95, which may affect the plasticity of excitatory synapses.
Journal ArticleDOI
Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
Colin J. Barnstable,Walter F. Bodmer,Geoffrey Brown,G. Galfre,C. Milstein,Alan F. Williams,Andreas Ziegler +6 more
TL;DR: The experiments established the usefulness of the bybrid myeloma technique in preparing monospecific antibodies against human cell surface antigens and highlights the possibilities not only of obtaining reagents for somatic cell genetics, but also of obtaining mouse antibodies detecting human antigenic polymorphisms.
References
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Journal ArticleDOI
Plaque Formation in Agar by Single Antibody-Producing Cells
Niels K. Jerne,Albert A. Nordin +1 more
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
Journal ArticleDOI
Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed Recombinants
TL;DR: Evidence can be obtained which suggests that mating may be followed by segregation in mice, and when two clonal lines of mouse fibroblasts are grown together for 4 days, hybrid cells can be detected by selective conditions.
Journal ArticleDOI
Progress in immunology: Syndromes of diminished resistance to infection
TL;DR: In this review of progress in immunology, an attempt is made to synthesize some of the rapidly accumulating observations in clinical medicine and experimental biology into a workable scheme, which may help pediatricians in their approach to the study and management of patients suspected of abnormalities in their resistance to infection.
Journal ArticleDOI
Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species.
Henry Harris,J. F. Watkins +1 more
TL;DR: Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different Species is presented, which describes how cells from different species can be hybrids.
Journal ArticleDOI
Mouse myelomas and lymphomas in culture
K. Horibata,A.W. Harris +1 more
TL;DR: Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells, suggesting that in these respects the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells.