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Journal ArticleDOI

Continuous cultures of fused cells secreting antibody of predefined specificity

07 Aug 1975-Nature (Nature)-Vol. 256, Iss: 5517, pp 495-497
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Abstract: THE manufacture of predefined specific antibodies by means of permanent tissue culture cell lines is of general interest. There are at present a considerable number of permanent cultures of myeloma cells1,2 and screening procedures have been used to reveal antibody activity in some of them. This, however, is not a satisfactory source of monoclonal antibodies of predefined specificity. We describe here the derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies. The cell lines are made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor. To understand the expression and interactions of the Ig chains from the parental lines, fusion experiments between two known mouse myeloma lines were carried out.
Citations
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Journal Article
TL;DR: In this article, a set of three monoclonal antibodies was described, each of which recognizes cells of the monocyte-macrophage lineage in the rat, and the tissue distribution, in particular in lymphoid organs, was determined by immunoenzyme histochemistry on cryostat sections, as well as on cell suspensions.
Abstract: In the present study, a set of three monoclonal antibodies is described, each of which recognizes cells of the monocyte-macrophage lineage in the rat. The tissue distribution, in particular in lymphoid organs, of each of the three monoclonals is determined by immunoenzyme histochemistry on cryostat sections, as well as on cell suspensions. Results show that ED1 recognizes a cytoplasmic antigen in monocytes and in most macrophages, free and fixed. ED2 and ED3 recognize membrane antigens of tissue macrophages, discriminating between distinct subpopulations of macrophages, each with a characteristic localization in the compartments of lymphoid organs. No other cell types except cells of the mononuclear phagocyte system are positive for any of the three monoclonals. Possible relations between the macrophages recognized by this set of monoclonals and dendritic cells are discussed.

1,718 citations

Journal ArticleDOI
16 Nov 1978-Nature
TL;DR: The identification of such a cell line, Sp2/0-Ag14, is reported here the identification of a tumour cell fusion partner that makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody.
Abstract: FUSION of myeloma cells which grow in tissue culture with spleen cells from an immunised mouse provides a general method for obtaining cell lines (hybridomas) which make antibody of the desired specificity1–3. Hybrids derived from these myelomas make the immunoglobulin (Ig) heavy and light chains of the myeloma parent as well as the antigen-specific heavy and light chains of the spleen cell parent. In conditions in which the two heavy and two light chains associate randomly, a hybridoma would make 10 distinct Ig molecules, and the specific antibody would comprise only 1/16 of the total Ig4,5. To obtain hybridomas making only the specific antibodies requires a tumour cell fusion partner that itself makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody. We report here the identification of such a cell line, Sp2/0-Ag14.

1,654 citations

Journal ArticleDOI
18 Feb 1993-Nature
TL;DR: A new radiolabelled ligand-binding assay, the molecularly im-printed sorbent assay, which uses antibody mimics, which accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique.
Abstract: Ligand-binding assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody, but antibodies require special handling and a costly production procedure. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline and diazepam showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly imprinted sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies.

1,613 citations

Journal ArticleDOI
TL;DR: A murine monoclonal antibody (OC125) has been developed that reacts with each of six epithelial ovarian carcinoma cell lines and with cryopreserved tumor tissue from 12 of 20 ovarian cancer patients, but does not bind to a variety of nonmalignant tissues, including adult and fetal ovary.
Abstract: A murine monoclonal antibody (OC125) has been developed that reacts with each of six epithelial ovarian carcinoma cell lines and with cryopreserved tumor tissue from 12 of 20 ovarian cancer patients. By contrast, the antibody does not bind to a variety of nonmalignant tissues, including adult and fetal ovary. OC125 reacts with only 1 of 14 cell lines derived from nonovarian neoplasms and has failed to react with cryostat sections from 12 nonovarian carcinomas.

1,609 citations

Journal ArticleDOI
TL;DR: A myeloma line has been developed which produces no globulin chains of its own, has a duplication of 8.7 h, fuses effectively with B-lymphoblasts and produces stable hybrids, and an enhancing effect of macrophages on hybridoma yields has been observed.

1,609 citations

References
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Journal ArticleDOI
26 Apr 1963-Science
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
Abstract: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.

2,273 citations

Journal ArticleDOI
14 Aug 1964-Science
TL;DR: Evidence can be obtained which suggests that mating may be followed by segregation in mice, and when two clonal lines of mouse fibroblasts are grown together for 4 days, hybrid cells can be detected by selective conditions.
Abstract: When two clonal lines of mouse fibroblasts, each containing a drug-resistant marker, are grown together for 4 days, hybrid cells can be detected by selective conditions. These hybrid cells are presumed to be the result of mating. By the same method evidence can be obtained which suggests that mating may be followed by segregation.

1,899 citations

Journal ArticleDOI
TL;DR: In this review of progress in immunology, an attempt is made to synthesize some of the rapidly accumulating observations in clinical medicine and experimental biology into a workable scheme, which may help pediatricians in their approach to the study and management of patients suspected of abnormalities in their resistance to infection.

581 citations

Journal ArticleDOI
13 Feb 1965-Nature
TL;DR: Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different Species is presented, which describes how cells from different species can be hybrids.
Abstract: Hybrid Cells Derived from Mouse and Man : Artificial Heterokaryons of Mammalian Cells from Different Species

562 citations

Journal ArticleDOI
TL;DR: Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells, suggesting that in these respects the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells.

491 citations