Coordination of Rho GTPase activities during cell protrusion
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894 citations
Cites background from "Coordination of Rho GTPase activiti..."
...This function of Rac could explain why Rac is most active slightly further back in the lamellipodium than RhoA (Machacek et al., 2009), although RhoA/ROCK can also phosphorylate and inhibit cofilin/ADF (Bernard, 2007)....
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...Using biosensors, active Rac1, RhoA, and Cdc42 have been shown to localize in lamellipodia during protrusion (Machacek et al., 2009)....
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746 citations
Cites background or methods from "Coordination of Rho GTPase activiti..."
...…4 • 2010 560 CFP variant optimized for FRET (Nguyen and Daugherty, 2005), and either the CRIB domain from p21-activated kinase (PBD) published previously (Machacek et al., 2009) or the Cdc42-binding CRIB domain from WASP (CBD), amino acids 230–314, fused to the C terminus of YPet, a YFP variant…...
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..., 2003; Hoppe and Swanson, 2004), but here is further optimized by the use of different fluorescent proteins and of a Cdc42-binding domain from WASP, a fragment shown to provide good selectivity for activated Cdc42 in a previously developed biosensor with a different design (Nalbant et al., 2004; Frantz et al., 2007; Machacek et al., 2009)....
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...JCB • VOLUME 188 • NUMBER 4 • 2010 560 CFP variant optimized for FRET (Nguyen and Daugherty, 2005), and either the CRIB domain from p21-activated kinase (PBD) published previously (Machacek et al., 2009) or the Cdc42-binding CRIB domain from WASP (CBD), amino acids 230–314, fused to the C terminus of YPet, a YFP variant optimized for FRET (Nguyen and Daugherty, 2005)....
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...The Rac1 FRET biosensor was reported previously (Kraynov et al., 2000; Machacek et al., 2009), and here includes modifications to improve FRET efficiency reported in Machacek et al. (2009)....
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...…is further optimized by the use of different fluorescent proteins and of a Cdc42-binding domain from WASP, a fragment shown to provide good selectivity for activated Cdc42 in a previously developed biosensor with a different design (Nalbant et al., 2004; Frantz et al., 2007; Machacek et al., 2009)....
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619 citations
Cites background from "Coordination of Rho GTPase activiti..."
...In addition to Rac, RhoA and Cdc42 are active in lamellipodial regions and contribute to lamellipodium extension [23,24]....
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References
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