CRISPR-Cas systems for diagnosing infectious diseases.
Anastasiya Kostyusheva,Sergey Brezgin,Yurii Babin,Irina Vasilyeva,Dieter Glebe,Dmitry Kostyushev,Vladimir Chulanov +6 more
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TLDR
A review on CRISPR-based technologies and their perspectives to become platforms for point-of-care nucleic acid detection methods and as deployable diagnostic platforms that could help to identify and curb outbreaks and emerging epidemics is presented in this paper.About:
This article is published in Methods.The article was published on 2021-04-09 and is currently open access. It has received 29 citations till now. The article focuses on the topics: CRISPR & Point-of-care testing.read more
Citations
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CRISPR/Cas-Based In Vitro Diagnostic Platforms for Cancer Biomarker Detection.
TL;DR: In this paper, the authors introduce the working mechanisms of different kinds of CRISPR/Cas systems for biosensing and CRISpl/Cas-mediated detection strategies for different types of cancer biomarkers including nucleic acids, proteins, and extracellular vesicles.
Journal ArticleDOI
Multivalent Duplexed-Aptamer Networks Regulated a CRISPR-Cas12a System for Circulating Tumor Cell Detection.
TL;DR: In this paper, a multivalent duplexed-aptamer networks (MDANs)-Cas12a-based CTC detection sensor was proposed, which was synthesized on a magnetic bead surface by rolling circle amplification (RCA).
Journal ArticleDOI
Micro-Electromechanical Systems-based Sensors and Their Applications
Sophia Nazir,Oh Seok Kwon +1 more
Journal ArticleDOI
Next-Generation Diagnostic with CRISPR/Cas: Beyond Nucleic Acid Detection
TL;DR: The technical challenges of integrating CRISPR/Cas technology into the onsite assessment of clinical and other specimens are highlighted, along with current improvements inCRISPR bio-sensing for nucleic acid and non-nucleic acid targets.
Journal ArticleDOI
CRISPR-Cas, Argonaute proteins and the emerging landscape of amplification-free diagnostics
Andrew Santiago-Frangos,Artem Nemudryi,Anna Nemudraia,Tanner Wiegand,Joseph Nichols,Pushya Krishna,Andrew M. Scherffius,Trevor Zahl,Royce A. Wilkinson,Blake Wiedenheft +9 more
TL;DR: In this paper , the authors introduce the origins of PCR and isothermal amplification, before turning to the emergence of CRISPR-Cas and Argonaute proteins, which are being coupled to fluorimeters, spectrometers, microfluidic devices, field effect transistors, and amperometric biosensors, for a new generation of nucleic acid based diagnostics.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
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The global distribution and burden of dengue
Samir Bhatt,Peter W. Gething,Oliver J. Brady,Jane P. Messina,Andrew Farlow,Catherine L. Moyes,John M. Drake,John M. Drake,John S. Brownstein,Anne G. Hoen,Osman Sankoh,Osman Sankoh,Monica F. Myers,Dylan B. George,Thomas Jaenisch,G. R. William Wint,Cameron P. Simmons,Thomas W. Scott,Thomas W. Scott,Jeremy Farrar,Jeremy Farrar,Simon I. Hay,Simon I. Hay +22 more
TL;DR: These new risk maps and infection estimates provide novel insights into the global, regional and national public health burden imposed by dengue and will help to guide improvements in disease control strategies using vaccine, drug and vector control methods, and in their economic evaluation.
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SARS and MERS: recent insights into emerging coronaviruses
TL;DR: The emergence of Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012 marked the second introduction of a highly pathogenic coronav virus into the human population in the twenty-first century, and the current state of development of measures to combat emerging coronaviruses is discussed.
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RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Wenyan Jiang,David Bikard,David Daniel Cox,David Daniel Cox,Feng Zhang,Feng Zhang,Luciano A. Marraffini +6 more
TL;DR: The exhaustively analyze dual-RNA:Cas9 target requirements to define the range of targetable sequences and show strategies for editing sites that do not meet these requirements, suggesting the versatility of this technique for bacterial genome engineering.
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