CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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Cites background from "CRISPR-Mediated Modular RNA-Guided ..."
...Specifically, fusion of dCas9 to effector domains including VP64 or KRAB allowed stable and efficient transcriptional activation or repression, respectively, in human and yeast cells (129)....
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Cites background from "CRISPR-Mediated Modular RNA-Guided ..."
...This CRISPR-based interference, or CRISPRi, works efficiently in prokaryotic genomes but is less effective in eukaryotic cells (Gilbert et al., 2013)....
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...…dCas9-mediated transcriptional repression needs to be further improved—in the current generation of dCas9-based eukaryotic transcription repressors, even the addition of helper functional domains results in only partial transcriptional knockdown (Gilbert et al., 2013; Konermann et al., 2013)....
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...The repressive function of CRISPRi can be enhanced by tethering dCas9 to transcriptional repressor domains such as KRAB or SID effectors, which promote epigenetic silencing (Gilbert et al., 2013; Konermann et al., 2013)....
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...However, dCas9-mediated transcriptional repression needs to be further improved—in the current generation of dCas9-based eukaryotic transcription repressors, even the addition of helper functional domains results in only partial transcriptional knockdown (Gilbert et al., 2013; Konermann et al., 2013)....
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"CRISPR-Mediated Modular RNA-Guided ..." refers background in this paper
...However, the remarkable success in using RNA-guided Cas9 to enable DNA cutting for many genes in distinct organisms argues that Cas9 can bind to target DNA sites in a broad range of genes or regulatory regions (Cho et al., 2013; Cong et al., 2013; DiCarlo et al., 2013; Gratz et al., 2013; Hwang et al., 2013; Jiang et al., 2013; Jinek et al., 2012, 2013; Mali et al., 2013; Wang et al., 2013)....
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...Recently, several groups have shown that a modified type II CRISPR (clustered regularly interspaced palindromic repeats) system can be targeted to DNA using RNA, enabling genetic editing of any region of the genome in a variety of organisms (Cho et al., 2013; Cong et al., 2013; DiCarlo et al., 2013; Gratz et al., 2013; Hwang et al., 2013; Jiang et al., 2013; Jinek et al., 2012, 2013; Mali et al., 2013; Wang et al., 2013)....
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...…distinct organisms argues that Cas9 can bind to target DNA sites in a broad range of genes or regulatory regions (Cho et al., 2013; Cong et al., 2013; DiCarlo et al., 2013; Gratz et al., 2013; Hwang et al., 2013; Jiang et al., 2013; Jinek et al., 2012, 2013; Mali et al., 2013; Wang et al., 2013)....
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...…can be targeted to DNA using RNA, enabling genetic editing of any region of the genome in a variety of organisms (Cho et al., 2013; Cong et al., 2013; DiCarlo et al., 2013; Gratz et al., 2013; Hwang et al., 2013; Jiang et al., 2013; Jinek et al., 2012, 2013; Mali et al., 2013; Wang et al., 2013)....
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