CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
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Cites methods from "CRISPR-SONIC: targeted somatic onco..."
...A similar technique was used to generate a model of ICC through integration of the oncogene HRAS in combination with deletion of p53.(91) The genetic alterations driving tumorigenesis are preceded by underlying environmental or inherited factors that damage the liver....
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References
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"CRISPR-SONIC: targeted somatic onco..." refers methods in this paper
...Guide RNAs targeting Actb and p53 were cloned into the pX330 (Addgene 42230) vectors using standard protocols and primers (Additional file 1: Table S1 and S2) [25]....
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"CRISPR-SONIC: targeted somatic onco..." refers background in this paper
...Background Genome editing has been revolutionized by the CRISPR/ Cas9 system [1]....
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...CRISPR/Cas9-mediated DSBs typically trigger one of two major pathways for DNA repair: (1) homology-independent repair, such as non-homologous end recombination (NHEJ), or (2) homology-directed repair (HDR) with an endogenous or exogenous homologous DNA template [1, 2]....
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