Crown monitoring: Trace the dynamic changes of caspase-3 and H2O2 in real-time imaging based on FRET/SERS.
TL;DR: In this paper, a dual-channel and real-time tracking of the dynamic changes in caspase-3 and hydrogen peroxide (H2O2) based on fluorescence resonance energy transfer (FRET)/surface-enhanced Raman spectroscopy (SERS) technology is presented.
About: This article is published in Biosensors and Bioelectronics.The article was published on 2021-08-03. It has received 10 citations till now. The article focuses on the topics: Nanoprobe.
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TL;DR: In this article , a dual stimulus-responsive core-satellite surface-enhanced Raman scattering (SERS) nanoprobe engineered with manganese dioxide (MnO2) and silver nanoparticles (Ag NPs) was constructed for sensitive H2O2 detection.
3 citations
TL;DR: In this paper , the authors summarize the applications of SERS combined with ML in recent years, such as the recognition of biological molecules, rapid diagnosis of diseases, developing of new immunoassay techniques, and enhancing SERS capabilities in semi-quantitative measurements.
Abstract: Abstract Surface‐enhanced Raman spectroscopy (SERS) has shown strength in non‐invasive, rapid, trace analysis and has been used in many fields in medicine. Machine learning (ML) is an algorithm that can imitate human learning styles and structure existing content with the knowledge to effectively improve learning efficiency. Integrating SERS and ML can have a promising future in the medical field. In this review, we summarize the applications of SERS combined with ML in recent years, such as the recognition of biological molecules, rapid diagnosis of diseases, developing of new immunoassay techniques, and enhancing SERS capabilities in semi‐quantitative measurements. Ultimately, the possible opportunities and challenges of combining SERS with ML are addressed.
3 citations
TL;DR: In this paper , the microstructure and SERS properties of alloy films were characterized by SEM, TEM, XRD and Raman spectroscopy, and a method for preparing SERS substrates with low cost, high sensitivity and excellent repeatability.
2 citations
TL;DR: In this paper , the authors provide a critical perspective on the fundamental and applied impact of incorporating DNAzymes in the field of electrochemical biosensing, highlighting recent advances in creating DNAzyme-based electrochemical sensors for diagnosing infectious diseases, cancer and regulatory diseases.
2 citations
TL;DR: In this article , a ratiometric electrochemical biosensing system is constructed for miRNAs detection by combining target triggered bipedal DNAzyme walker cleavage cycling amplification and planar intercalated methylene blue (MB) molecules amplification.
2 citations
References
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Donostia International Physics Center1, Rovira i Virgili University2, MacDiarmid Institute for Advanced Materials and Nanotechnology3, Victoria University of Wellington4, University of Cambridge5, University of California, Santa Barbara6, Queen's University Belfast7, Technical University of Denmark8, University of Victoria9, Chung-Ang University10, University of Jena11, Leibniz Institute of Photonic Technology12, Rutgers University13, University of Strathclyde14, University of Liverpool15, University of Iowa16, University of Minnesota17, Heidelberg University18, National Institute of Advanced Industrial Science and Technology19, Chalmers University of Technology20, Humboldt University of Berlin21, University of Michigan22, Jiangnan University23, Stanford University24, Xiamen University25, Ludwig Maximilian University of Munich26, Hokkaido University27, Seoul National University28, University of Illinois at Urbana–Champaign29, Kwansei Gakuin University30, University of Vigo31, Free University of Berlin32, Northwestern University33, University of Duisburg-Essen34, National Research Council35, Indian Institute of Science Education and Research, Thiruvananthapuram36, Duke University37, Northeastern University (China)38, Temple University39, Wuhan University40, Japan Advanced Institute of Science and Technology41, Jilin University42, Ikerbasque43
TL;DR: Prominent authors from all over the world joined efforts to summarize the current state-of-the-art in understanding and using SERS, as well as to propose what can be expected in the near future, in terms of research, applications, and technological development.
Abstract: The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article.
1,768 citations
TL;DR: The physiological implications of cells having to maintain a reducing environment during apoptosis to allow the caspases to function are discussed and hydrogen peroxide has two distinct effects.
666 citations
TL;DR: The combined use of modern instrumental analytical approaches has unravelled the ideal outcomes in metabolomics, and is beneficial to increase the coverage of detected metabolites that can not be achieved by single-analysis techniques.
Abstract: Metabolomics is the comprehensive assessment of endogenous metabolites and attempts to systematically identify and quantify metabolites from a biological sample. Small-molecule metabolites have an important role in biological systems and represent attractive candidates to understand disease phenotypes. Metabolites represent a diverse group of low-molecular-weight structures including lipids, amino acids, peptides, nucleic acids, organic acids, vitamins, thiols and carbohydrates, which makes global analysis a difficult challenge. The recent rapid development of a range of analytical platforms, including GC, HPLC, UPLC, CE coupled to MS and NMR spectroscopy, could enable separation, detection, characterization and quantification of such metabolites and related metabolic pathways. Owing to the complexity of the metabolome and the diverse properties of metabolites, no single analytical platform can be applied to detect all metabolites in a biological sample. The combined use of modern instrumental analytical approaches has unravelled the ideal outcomes in metabolomics, and is beneficial to increase the coverage of detected metabolites that can not be achieved by single-analysis techniques. Integrated platforms have been frequently used to provide sensitive and reliable detection of thousands of metabolites in a biofluid sample. Continued development of these analytical platforms will accelerate widespread use and integration of metabolomics into systems biology. Here, the application of each hyphenated technique is discussed and its strengths and limitations are discussed with selected illustrative examples; furthermore, this review comprehensively highlights the role of integrated tools in metabolomic research.
663 citations
TL;DR: A ROS-dependent form of cell death, ‘oxeiptosis’, that resembles apoptosis but uses a pathway distinct from all previously described death pathways is demonstrated.
Abstract: Reactive oxygen species (ROS) are generated by virus-infected cells; however, the physiological importance of ROS generated under these conditions is unclear. Here we found that the inflammation and cell death induced by exposure of mice or cells to sources of ROS were not altered in the absence of canonical ROS-sensing pathways or known cell-death pathways. ROS-induced cell-death signaling involved interactions among the cellular ROS sensor and antioxidant factor KEAP1, the phosphatase PGAM5 and the proapoptotic factor AIFM1. Pgam5 -/- mice showed exacerbated lung inflammation and proinflammatory cytokines in an ozone-exposure model. Similarly, challenge with influenza A virus led to increased infiltration of the virus, lymphocytic bronchiolitis and reduced survival of Pgam5 -/- mice. This pathway, which we have called 'oxeiptosis', was a ROS-sensitive, caspase independent, non-inflammatory cell-death pathway and was important for protection against inflammation induced by ROS or ROS-generating agents such as viral pathogens.
184 citations
TL;DR: It is suggested that strong oxidants generate intracellular ROS and ceramide which in term lead to down-regulation of Akt by dephosphorylation and caspase-3-independent proteolysis.
166 citations