Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-A resolution containing a full complement of coppers.
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This work presents the first crystal structure of an active laccase containing a full complement of coppers, the complete polypeptide chain together with seven carbohydrate moieties, and a mechanism is presented to explain how laccases could tune their redox potential by as much as 200 mV.About:
This article is published in Journal of Biological Chemistry.The article was published on 2002-10-04 and is currently open access. It has received 817 citations till now. The article focuses on the topics: Laccase & Multicopper oxidase.read more
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Journal ArticleDOI
Fungal laccases - occurrence and properties.
TL;DR: The fact that laccases only require molecular oxygen for catalysis makes them suitable for biotechnological applications for the transformation or immobilization of xenobiotic compounds.
Journal ArticleDOI
Copper Active Sites in Biology
Edward I. Solomon,David E. Heppner,Esther M. Johnston,Jake W. Ginsbach,Jordi Cirera,Munzarin F. Qayyum,Matthew T. Kieber-Emmons,Christian H. Kjaergaard,Ryan G. Hadt,Li Tian +9 more
TL;DR: This review presents in depth discussions of all these classes of Cu enzymes and the correlations within and among these classes, as well as the present understanding of the enzymology, kinetics, geometric structures, electronic structures and the reaction mechanisms these have elucidated.
Journal ArticleDOI
Laccases: blue enzymes for green chemistry
TL;DR: A brief discussion of laccases, a group of enzymes that work with air and produce water as the only by-product, and their uses span from the textile to the pulp and paper industries, and from food applications to bioremediation processes.
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Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin.
Ángel T. Martínez,Mariela Speranza,Francisco J. Ruiz-Dueñas,Patricia Ferreira,Susana Camarero,Francisco Guillén,Francisco Guillén,María Jesús Martínez,Ana Gutiérrez,José C. del Río +9 more
TL;DR: Broadening the knowledge of lignocellulose biodegradation processes should contribute to better control of wood-decaying fungi, as well as to the development of new biocatalysts of industrial interest based on these organisms and their enzymes.
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Protein-inorganic hybrid nanoflowers
TL;DR: A method is reported for creating hybrid organic-inorganic nanoflowers using copper (II) ions as the inorganic component and various proteins as the organic component to exhibit enhanced enzymatic activity and stability compared with the free enzyme.
References
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Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
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The CCP4 suite: programs for protein crystallography
TL;DR: The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography.
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MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures
TL;DR: The MOLSCRIPT program as discussed by the authors produces plots of protein structures using several different kinds of representations, including simple wire models, ball-and-stick models, CPK models and text labels.
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Improved methods for building protein models in electron density maps and the location of errors in these models.
TL;DR: In this paper, the authors describe strategies and tools that help to alleviate this problem and simplify the model-building process, quantify the goodness of fit of the model on a per-residue basis and locate possible errors in peptide and side-chain conformations.
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Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons.
TL;DR: It is demonstrated in this work that the surface tension, water‐organic solvent, transfer‐free energies and the thermodynamics of melting of linear alkanes provide fundamental insights into the nonpolar driving forces for protein folding and protein binding reactions.