Journal ArticleDOI
Cytogenetic and DNA fingerprinting analysis in three species of Swertia from Eastern Himalaya
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TLDR
Chromosome analysis of these three Swertia species showed that the majority of cells were diploid with zygotic chromosome number 2n = 26 and gametic chromosome number n = 13, and no variation was observed in pollen mother cells (PMCs) during meiosis.Abstract:
The present study reports detailed and comparative karyomorphological and meiotic analyses in Swertia chirayita, S. nervosa and S. bimaculata of Eastern Himalaya. Chromosome analysis of these three Swertia species showed that the majority of cells were diploid with zygotic chromosome number 2n = 26 and gametic chromosome number n = 13. The mosaic chromosome number counts in root tip cells ranged from 9–24 chromosomes in S. bimaculata, to 11–24 chromosomes in both S. chirayita and S. nervosa. No variation was observed in pollen mother cells (PMCs) during meiosis. The modal karyotypes were determined as 2M + 12m + 10sm + 2m:sm for S. chirayita, 8M + 14m + 2sm + 2m:sm for S. nervosa and 6M + 16m + 2sm + 2M:st for S. bimaculata. Karyomorphological analysis of the three Swertia species have documented some distinct similarities between S. chirayita and S. nervosa in terms of their chromosomal category (small), mean chromosomal length (2.48μm, 2.62 μm), length of total chromatin (32.18μm, 34.14 μm) as well as p...read more
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Assessment of genetic diversity among different population of five Swertia species by using molecular and phytochemical markers
TL;DR: Assessment of molecular and phytochemical diversity among 48 accessions of five Swertia species collected from Western Himalayas, India finds the potent S. chirata displays a low degree of genetic diversity and gene flow, and therefore there is a major risk of extinction of this significant species.
Journal ArticleDOI
Biotechnological interventions and genetic diversity assessment in Swertia sp.: a myriad source of valuable secondary metabolites.
Prabhjot Kaur,Devendra Kumar Pandey,R. C. Gupta,Vijay Kumar,Padmanabh Dwivedi,Rupa Sanyal,Abhijit Dey +6 more
TL;DR: A comprehensive assessment on biotechnological process made in Swertia over the past few years is presented in this paper, where the authors present a comprehensive assessment of the biotechnology process made by the authors.
Journal ArticleDOI
Genetic diversity in Nepalese population of Swertia chirayita (Roxb. Ex Fleming) H. Karst based on inter-simple sequence repeats (ISSR) markers
TL;DR: The present genetic diversity assessment of S. chirayita populations has been of immense importance to understand the cause of its vulnerability and has furnished valuable insights for its conservation and sustainable utilization.
References
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
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A rapid DNA isolation procedure for small quantities of fresh leaf tissue
Edwin Pahlich,Chr. Gerlitz +1 more
TL;DR: From the kinetic data, it becomes evident that the reductive amination reaction is highly adaptive to the ammonium environment.
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI
Nomenclature for centromeric position on chromosomes
TL;DR: At the present time, with the immense increase in research activity in mammalian cytology, the terminology of the centromeric position has become burdened by much obscurity and confusion.