Cytotoxic phenolics from Bulbophyllum odoratissimum
TL;DR: A phytochemical study of the whole plant of Bulbophyllum odoratissimum (J.E. Smith) Lindl (Orchidaceae) and cytotoxicity measurement of its isolated compounds have been carried out and all compounds, except for 9, have not been reported in this plant before.
About: This article is published in Food Chemistry.The article was published on 2008-03-01. It has received 66 citations till now.
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TL;DR: It is demonstrated that 1 suppresses the migratory behavior of non-small cell lung cancer H460 cells and the inhibitory activity of 1 on lung cancer migration suggests that this compound may be suitable for further development for the treatment of cancer metastasis.
Abstract: Lung cancer is one of the most common causes of cancer death due to its high metastasis potential. The process of cancer migration is an early step that is required for successful metastasis. The discovery and development of natural compounds for cancer therapy have garnered increasing attention in recent years. Gigantol (1) is a bibenzyl compound derived from the Thai orchid, Dendrobium draconis. It exhibits significant cytotoxic activity against several cancer cell lines; however, until recently, the role of 1 on tumor metastasis has not been characterized. This study demonstrates that 1 suppresses the migratory behavior of non-small cell lung cancer H460 cells. Western blot analysis reveals that 1 down-regulates caveolin-1 (Cav-1), activates ATP-dependent tyrosine kinase (phosphorylated Akt at Ser 473), and cell division cycle 42 (Cdc42), thereby suppressing filopodia formation. The inhibitory effect of 1 on cell movement is also exhibited in another lung cancer cell line, H292, but not in normal human...
76 citations
TL;DR: In this article, a methanol extract from the whole plant of Dendrobium formosum Roxb. ex Lindl, Orchidaceae, showed inhibitory potential against α-glucosidase and pancreatic lipase enzymes.
Abstract: A methanol extract from the whole plant of Dendrobium formosum Roxb. ex Lindl., Orchidaceae, showed inhibitory potential against α-glucosidase and pancreatic lipase enzymes. Chromatographic separation of the extract resulted in the isolation of twelve phenolic compounds. The structures of these compounds were determined through analysis of NMR and HR-ESI-MS data. All of the isolates were evaluated for their α-glucosidase and pancreatic lipase inhibitory activities, as well as glucose uptake stimulatory effect. Among the isolates, 5-methoxy-7-hydroxy-9,10-dihydro-1,4-phenanthrenequinone (12) showed the highest α-glucosidase and pancreatic lipase inhibitory effects with an IC50 values of 126.88 ± 0.66 μM and 69.45 ± 10.14 μM, respectively. An enzyme kinetics study was conducted by the Lineweaver-Burk plot method. The kinetics studies revealed that compound 12 was a non-competitive inhibitor of α-glucosidase and pancreatic lipase enzymes. Moreover, lusianthridin at 1 and 10 μg/ml and moscatilin at 100 μg/ml showed glucose uptake stimulatory effect without toxicity on L6 myotubes. This study is the first report on the phytochemical constituents and anti-diabetic and anti-obesity activities of D. formosum.
68 citations
TL;DR: Phytochemical investigation of the ethyl acetate fraction of tartary buckwheat roots led to the isolation of seven new phenylpropanoid glycosides, tatarisides A-G, which were evaluated for their cytotoxic activity against four human cancer cell lines.
55 citations
Cites methods from "Cytotoxic phenolics from Bulbophyll..."
...The MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazoliumbr omide] colourimetric assay, against A-549, HCT116, ZR-75-30 and HL-60, was performed as described in the literature with minor modification (Chen et al., 2008; Korolyov et al., 2010)....
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TL;DR: The isolated compounds showed appreciable cytotoxicity against human lung cancer cell lines with IC50 values of 196.7, 23.4, 65.0, and 125.8 μg/mL, respectively, and exhibited antimigratory property at nontoxic concentrations.
Abstract: Chromatographic separation of a methanol extract prepared from the whole plant of Dendrobium brymerianum led to the isolation of eight phenolic compounds. Among the isolated compounds (1–8), moscatilin (1), gigantol (3), lusianthridin (4), and dendroflorin (6) showed appreciable cytotoxicity against human lung cancer cell lines with IC50 values of 196.7, 23.4, 65.0, and 125.8 μg/mL, respectively, and exhibited antimigratory property at nontoxic concentrations. This study is the first report on the biological activities of this plant.
54 citations
Cites background from "Cytotoxic phenolics from Bulbophyll..."
...this MeOH extract resulted in the isolation of eight phenolic compounds which included moscatilin (1) [19], flavanthrinin (2) [20], gigantol (3) [21], lusianthridin (4) [22], nobilone (5) [23], dendroflorin (6) [24], denchrysan B (7) [25], and tristin (8) [21]....
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TL;DR: The results indicated that compounds 5c, 6c and 6h inhibit S(180) and H(22) growth and have very low acute toxicities, and the preliminary analysis of structure-activity relationships is discussed.
52 citations
References
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TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
50,114 citations
"Cytotoxic phenolics from Bulbophyll..." refers methods in this paper
...Growth inhibition by the sample of tumour cells was measured by microculture tetrazolium (MTT) assay, with minor modification (Alley et al., 1988; Mosmann, 1983; Zhou, Yue, Han, & Yang, 1993)....
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Journal Article•
TL;DR: Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
Abstract: For the past 30 years strategies for the preclinical discovery and development of potential anticancer agents have been based largely upon the testing of agents in mice bearing transplantable leukemias and solid tumors derived from a limited number of murine as well as human sources. The feasibility of implementing an alternate approach, namely combined in vitro/in vivo screening for selective cytotoxicity among panels of human tumor cell lines derived from a broad spectrum of human solid tumors is under investigation. A group of 30 cell lines acquired from a variety of sources and representing 8 lung cancer pathologies as well as 76 cell lines representing 10 other categories of human cancer (carcinomas of colon, breast, kidney, prostate, ovary, head and neck; glioma; leukemia; melanoma; and sarcoma) have exhibited acceptable growth characteristics and suitable colorimetric profiles in a single, standard culture medium. Measurements of in vitro growth in microculture wells by cell-mediated reduction of tetrazolium showed excellent correlation (0.89 less than r2 less than 0.98) with measurements of cellular protein in adherent cell line cultures as well as viable cell count in suspension cell line cultures (0.94 less than r2 less than 0.99). Since the microculture tetrazolium assay provides sensitive and reproducible indices of growth as well as drug sensitivity in individual cell lines over the course of multiple passages and several months' cultivation, it appears suitable for initial-stage in vitro drug screening.
3,098 citations
"Cytotoxic phenolics from Bulbophyll..." refers methods in this paper
...Growth inhibition by the sample of tumour cells was measured by microculture tetrazolium (MTT) assay, with minor modification (Alley et al., 1988; Mosmann, 1983; Zhou, Yue, Han, & Yang, 1993)....
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TL;DR: Among the identified compounds, five were found to exhibit anti-platelet aggregation activity in vitro and the structure of a previously reported compound dendroflorin obtained from the plant was revised on the basis of spectroscopic analysis.
143 citations
TL;DR: Two phenanthrenes were isolated from the aerial part of Dendrobium nobile Lindl and were found to be cytotoxic against A549, SK-OV-3, and HL-60 cell lines and antitumor activity on the life span of ICR mice intraperitoneally implanted with 1 x 10(6) cells of sarcoma 180.
Abstract: Two phenanthrenes were isolated from the aerial part of Dendrobium nobile Lindl. and their structures were identified to be 4,7-dihydroxy-2-methoxy-9,10-dihydrophenanthrene (1) and denbinobin (2), among which the former has been first isolated from this plant. These two compounds were found to be cytotoxic against A549 (human lung carcinoma), SK-OV-3 (human ovary adenocarcinoma), and HL-60 (human promyelocytic leukemia) cell lines. Compound 1 also showed antitumor activity on the life span of ICR mice intraperitoneally implanted with 1 x 10(6) cells of sarcoma 180.
130 citations
TL;DR: Erianin caused extensive tumour necrosis, growth delay and rapid vascular shutdown in hepatoma and melanoma models; it inhibited angiogenesis in vivo and in vitro and induced endothelial cytoskeletal disorganisation.
118 citations
"Cytotoxic phenolics from Bulbophyll..." refers background in this paper
...As several phenanthrenes and bibenzyls from plants of Orchidaceae were found to possess anti-tumour activity (Gong et al., 2004; Lee, Park, Baek, Kim, & Ahn, 1995; Lin et al., 2001), the isolated compounds were evaluated in vitro for their inhibitory ability against the growth of human leukaemia cell lines K562 and HL-60, human lung adenocarcinoma A549, human hepatoma BEL-7402 and human stomach cancer SGC-7901, using cisplatin as a positive control....
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...As several phenanthrenes and bibenzyls from plants of Orchidaceae were found to possess anti-tumour activity (Gong et al., 2004; Lee, Park, Baek, Kim, & Ahn, 1995; Lin et al., 2001), the isolated compounds were evaluated in vitro for their inhibitory ability against the growth of human leukaemia…...
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