Journal ArticleDOI
Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing
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TLDR
It is estimated that transcripts from ∼95% of multiexon genes undergoAlternative splicing and that there are ∼100,000 intermediate- to high-abundance alternative splicing events in major human tissues.Abstract:
We carried out the first analysis of alternative splicing complexity in human tissues using mRNA-Seq data. New splice junctions were detected in approximately 20% of multiexon genes, many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data, we estimate that transcripts from approximately 95% of multiexon genes undergo alternative splicing and that there are approximately 100,000 intermediate- to high-abundance alternative splicing events in major human tissues. From a comparison with quantitative alternative splicing microarray profiling data, we also show that mRNA-Seq data provide reliable measurements for exon inclusion levels.read more
Citations
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Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation
TL;DR: A flexible statistical framework is developed for the analysis of read counts from RNA-Seq gene expression studies, and parallel computational approaches are developed to make non-linear model fitting faster and more reliable, making the application of GLMs to genomic data more convenient and practical.
Journal ArticleDOI
UniProt: the universal protein knowledgebase in 2021
Alex Bateman,Maria Jesus Martin,Sandra Orchard,Michele Magrane,Rahat Agivetova,Shadab Ahmad,Emanuele Alpi,Emily H Bowler-Barnett,Ramona Britto,Borisas Bursteinas,Hema Bye-A-Jee,Ray Coetzee,Austra Cukura,Alan Wilter Sousa da Silva,Paul Denny,Tunca Doğan,ThankGod Ebenezer,Jun Fan,Leyla Jael Garcia Castro,Penelope Garmiri,George Georghiou,Leonardo Gonzales,Emma Hatton-Ellis,Abdulrahman Hussein,Alexandr Ignatchenko,Giuseppe Insana,Rizwan Ishtiaq,Petteri Jokinen,Vishal Joshi,Dushyanth Jyothi,Antonia Lock,Rodrigo Lopez,Aurelien Luciani,Jie Luo,Yvonne Lussi,Alistair MacDougall,Fábio Madeira,Mahdi Mahmoudy,Manuela Menchi,Alok Mishra,Katie Moulang,Andrew Nightingale,Carla Susana Oliveira,Sangya Pundir,Guoying Qi,Shriya Raj,Daniel Rice,Milagros Rodriguez Lopez,Rabie Saidi,Joseph Sampson,Tony Sawford,Elena Speretta,Edward Turner,Nidhi Tyagi,Preethi Vasudev,Vladimir Volynkin,Kate Warner,Xavier Watkins,Rossana Zaru,Hermann Zellner,Alan Bridge,Sylvain Poux,Nicole Redaschi,Lucila Aimo,Ghislaine Argoud-Puy,Andrea H. Auchincloss,Kristian B. Axelsen,Parit Bansal,Delphine Baratin,Marie-Claude Blatter,Jerven Bolleman,Emmanuel Boutet,Lionel Breuza,Cristina Casals-Casas,Edouard de Castro,Kamal Chikh Echioukh,Elisabeth Coudert,Béatrice A. Cuche,M Doche,Dolnide Dornevil,Anne Estreicher,Maria Livia Famiglietti,Marc Feuermann,Elisabeth Gasteiger,Sebastien Gehant,Vivienne Baillie Gerritsen,Arnaud Gos,Nadine Gruaz-Gumowski,Ursula Hinz,Chantal Hulo,Nevila Hyka-Nouspikel,Florence Jungo,Guillaume Keller,Arnaud Kerhornou,Vicente Lara,Philippe Le Mercier,Damien Lieberherr,Thierry Lombardot,Xavier D. Martin,Patrick Masson,Anne Morgat,Teresa Batista Neto,Salvo Paesano,Ivo Pedruzzi,Sandrine Pilbout,Lucille Pourcel,Monica Pozzato,Manuela Pruess,Catherine Rivoire,Christian J. A. Sigrist,K Sonesson,Andre Stutz,Shyamala Sundaram,Michael Tognolli,Laure Verbregue,Cathy H. Wu,Cecilia N. Arighi,Leslie Arminski,Chuming Chen,Yongxing Chen,John S. Garavelli,Hongzhan Huang,Kati Laiho,Peter B. McGarvey,Darren A. Natale,Karen E. Ross,C. R. Vinayaka,Qinghua Wang,Yuqi Wang,Lai-Su L. Yeh,Jian Zhang,Patrick Ruch,Douglas Teodoro +132 more
TL;DR: The UniProtKB responded to the COVID-19 pandemic through expert curation of relevant entries that were rapidly made available to the research community through a dedicated portal and a credit-based publication submission interface was developed.
Journal ArticleDOI
An RNA-Sequencing Transcriptome and Splicing Database of Glia, Neurons, and Vascular Cells of the Cerebral Cortex
Ye Zhang,Kenian Chen,Steven A. Sloan,Mariko L. Bennett,Anja R. Scholze,Sean O'Keeffe,Hemali Phatnani,Paolo Guarnieri,Christine Caneda,Nadine Ruderisch,Shuyun Deng,Shane A. Liddelow,Chaolin Zhang,Richard Daneman,Tom Maniatis,Ben A. Barres,Jian Qian Wu +16 more
TL;DR: The authors' data provide clues as to how neurons and astrocytes differ in their ability to dynamically regulate glycolytic flux and lactate generation attributable to unique splicing of PKM2, the gene encoding the glycoleytic enzyme pyruvate kinase.
Journal ArticleDOI
Differential analysis of gene regulation at transcript resolution with RNA-seq
Cole Trapnell,David G. Hendrickson,David G. Hendrickson,Martin Sauvageau,Martin Sauvageau,Loyal A. Goff,Loyal A. Goff,John L. Rinn,John L. Rinn,Lior Pachter +9 more
TL;DR: Cuffdiff 2, an algorithm that estimates expression at transcript-level resolution and controls for variability evident across replicate libraries, robustly identifies differentially expressed transcripts and genes and reveals differential splicing and promoter-preference changes.
Journal ArticleDOI
mRNA-Seq whole-transcriptome analysis of a single cell.
Fuchou Tang,Catalin Barbacioru,Yangzhou Wang,Ellen Nordman,Clarence Lee,Nanlan Xu,Xiaohui Wang,John Bodeau,Brian B. Tuch,Asim Siddiqui,Kaiqin Lao,M. Azim Surani +11 more
TL;DR: A single-cell digital gene expression profiling assay with only a single mouse blastomere is described, which detected the expression of 75% more genes than microarray techniques and identified 1,753 previously unknown splice junctions called by at least 5 reads.
References
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Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Mapping and quantifying mammalian transcriptomes by RNA-Seq.
TL;DR: Although >90% of uniquely mapped reads fell within known exons, the remaining data suggest new and revised gene models, including changed or additional promoters, exons and 3′ untranscribed regions, as well as new candidate microRNA precursors.
Journal ArticleDOI
BLAT—The BLAST-Like Alignment Tool
TL;DR: How BLAT was optimized is described, which is more accurate and 500 times faster than popular existing tools for mRNA/DNA alignments and 50 times faster for protein alignments at sensitivity settings typically used when comparing vertebrate sequences.
Journal ArticleDOI
A gene atlas of the mouse and human protein-encoding transcriptomes
Andrew I. Su,Tim Wiltshire,Serge Batalov,Hilmar Lapp,Keith A. Ching,David Block,Jie Zhang,Richard Soden,Mimi Hayakawa,Gabriel Kreiman,Gabriel Kreiman,Michael P. Cooke,John R. Walker,John B. Hogenesch,John B. Hogenesch +14 more
TL;DR: In this paper, high-density oligonucleotide arrays offer the opportunity to examine patterns of gene expression on a genome scale, and the authors have designed custom arrays that interrogate the expression of the vast majority of proteinencoding human and mouse genes and have used them to profile a panel of 79 human and 61 mouse tissues.
Journal ArticleDOI
Molecular diversity of myofibrillar proteins: gene regulation and functional significance
TL;DR: The pattern of isogene expression varies during muscle development in relation to the different origin of myogenic cells and primary/secondary fiber generations and is affected by neural and hormonal influences.