Journal ArticleDOI
Delivery of dsRNA for RNAi in insects: an overview and future directions
Na Yu,Olivier Christiaens,Jisheng Liu,Jinzhi Niu,Kaat Cappelle,Silvia Caccia,Hanneke Huvenne,Guy Smagghe +7 more
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TLDR
The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research.Abstract:
RNA interference (RNAi) refers to the process of exogenous double-stranded RNA (dsRNA) silencing the complementary endogenous messenger RNA. RNAi has been widely used in entomological research for functional genomics in a variety of insects and its potential for RNAi-based pest control has been increasingly emphasized mainly because of its high specificity. This review focuses on the approaches of introducing dsRNA into insect cells or insect bodies to induce effective RNAi. The three most common delivery methods, namely, microinjection, ingestion, and soaking, are illustrated in details and their advantages and limitations are summarized for purpose of feasible RNAi research. In this review, we also briefly introduce the two possible dsRNA uptake machineries, other dsRNA delivery methods and the history of RNAi in entomology. Factors that influence the specificity and efficiency of RNAi such as transfection reagents, selection of dsRNA region, length, and stability of dsRNA in RNAi research are discussed for further studies.read more
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Journal ArticleDOI
Towards the elements of successful insect RNAi.
Jeffrey G. Scott,Kristin Michel,Lyric C. Bartholomay,Blair D. Siegfried,Wayne B. Hunter,Guy Smagghe,Kun Yan Zhu,Angela E. Douglas +7 more
TL;DR: An enhanced conceptual understanding ofRNAi function in insects will facilitate the application of RNAi for dissection of gene function, and to fast-track the application to both control pests and develop effective methods to protect beneficial insects and non-insect arthropods from viral and parasitic diseases.
Journal ArticleDOI
Silencing of Aphid Genes by dsRNA Feeding from Plants
Marco Pitino,Alexander D. Coleman,Massimo E. Maffei,Christopher J. Ridout,Saskia A. Hogenhout +4 more
TL;DR: The developed plant-mediated RNAi technology for aphids to allow for gene silencing in the aphid natural environment and minimize handling of these insects during experiments demonstrates the feasibility of the plant- mediated RNAi approach for aphid control.
Journal ArticleDOI
Ecology, Worldwide Spread, and Management of the Invasive South American Tomato Pinworm, Tuta absoluta: Past, Present, and Future
TL;DR: The South American tomato pinworm, Tuta absoluta (Meyrick), is native to the western Neotropics and has become a major threat to world tomato production.
Journal ArticleDOI
Insect immunology and hematopoiesis.
TL;DR: How the immune and circulatory systems have co-adapted to combat infection, how hemocyte replication and differentiation takes place (hematopoiesis), how an infection prepares an insect for a subsequent infection (immune priming), how environmental factors such as temperature and the age of the insect impact the immune response, and how social immunity protects entire groups are discussed.
Journal ArticleDOI
The next generation of insecticides: dsRNA is stable as a foliar-applied insecticide.
Keri San Miguel,Jeffrey G. Scott +1 more
TL;DR: It is confirmed that foliar application of Colorado potato beetle dsRNA actin is highly effective for control, demonstrated that treatment with actin-dsRNA protects potato plants for at least 28 days under greenhouse conditions and found that the ds RNA is not readily removed by water once dried on the leaves.
References
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TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Journal ArticleDOI
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TL;DR: The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
Journal ArticleDOI
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Georg Dietzl,Doris Chen,Frank Schnorrer,Kuan-Chung Su,Yulia Barinova,Michaela Fellner,Michaela Fellner,Beate Gasser,Kaolin Kinsey,Kaolin Kinsey,Silvia Oppel,Silvia Oppel,Susanne Scheiblauer,Africa Couto,Vincent Marra,Krystyna Keleman,Krystyna Keleman,Barry J. Dickson,Barry J. Dickson +18 more
TL;DR: The generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism and opening up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophile lifespan.
Journal ArticleDOI
Specific interference by ingested dsRNA
Lisa Timmons,Andrew Fire +1 more
TL;DR: This work shows that C. elegans can respond in a gene-specific manner to dsRNA encountered in the environment, and finds that Escherichia coli bacteria expressing dsRNAs can confer specific interference effects on the nematode larvae that feed on them.
Journal ArticleDOI
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
TL;DR: An efficient induction of RNAi using bacteria to deliver double-stranded RNA is reported, found to be most effective in inducing RNAi for non-neuronal tissue of late larval and adult hermaphrodites, with decreased effectiveness in the nervous system, in early larval stages, and in males.
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