Journal ArticleDOI
Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
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TLDR
In this article, the development of a new class of nucleoside phosphites is described, which are stable to normal laboratory conditions, are activated by mild acid treatment, and are observed to react essentially quantitatively with protected nucleosides.About:
This article is published in Tetrahedron Letters.The article was published on 1981-01-01. It has received 2299 citations till now. The article focuses on the topics: Nucleoside phosphoramidite.read more
Citations
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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
TL;DR: In adding the restriction endonuclease cleavage sites for SphI and KpnI to the lac cloning region of the phage vectors, this technique can be used as a general method for inserting sequences of DNA as well as introducing deletions and base pair changes.
Journal ArticleDOI
Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.
Rik Derynck,Julie A. Jarrett,Ellson Y. Chen,Dennis H. Eaton,John Richard Bell,Richard K. Assoian,Anita B. Roberts,Michael B. Sporn,David V. Goeddel +8 more
TL;DR: The cDNA sequence indicates that the 112-amino acid monomeric form of the natural TGF-β homodimer is derived proteolytically from a much longer precursor polypeptide which may be secreted.
Journal ArticleDOI
A cellular gene encodes scrapie PrP 27-30 protein
Bruno Oesch,Bruno Oesch,David Westaway,Monika Wälchli,Monika Wälchli,Michael P. McKinley,Stephen B. H. Kent,Stephen B. H. Kent,Ruedi Aebersold,Ruedi Aebersold,Ronald A. Barry,Paul Tempst,Paul Tempst,David B. Teplow,David B. Teplow,Leroy Hood,Leroy Hood,Stanley B. Prusiner,Stanley B. Prusiner,Charles Weissmann,Charles Weissmann +20 more
TL;DR: A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein.
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Antisense oligonucleotides as therapeutic agents--is the bullet really magical?
Cy A. Stein,Yung-Chi Cheng +1 more
TL;DR: The potential use of phosphorothioate oligos as inhibitors of viral replication is highlighted and these are examples of oligos that are being considered for clinical therapeutic trials and meet some, but not all, of these criteria.
PatentDOI
Peptide nucleic acids
TL;DR: A peptide nucleic acid (PNA) as discussed by the authors is a class of compounds that can bind complementary ssDNA and RNA strands more strongly than a corresponding DNA, and it can be used to attach DNA bases to a peptide backbone through a suitable linker.
References
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Synthesis of thymidine oligonucleotides by phosphite triester intermediates
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The synthesis of oligodeoxyprimidines on a polymer support
TL;DR: In this paper, a polymer supported method for synthesizing deoxypolyn nucleotides was described, and two non-anucleotides were synthesized in a four-hour time.
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Letter: Phosphite coupling procedure for generating internucleotide links.
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Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates
G. W. Daub,E. E. Van Tamelen +1 more
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Letter: Levulinic esters. An alcohol protecting group applicable to some nucleosides.
TL;DR: Results show that aprotic solvents on the F NMR substituent shifts can be shown on the basis of relatively small field effect contributions: S. Dewar and Y. Takeuchi, ibid.