Journal ArticleDOI
Design of the blood group AB glycotope.
Elena Korchagina,Tatyana V. Pochechueva,Polina Obukhova,Andrey A. Formanovsky,Anne Imberty,Robert Rieben,N. V. Bovin +6 more
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TLDR
A polymeric conjugate is synthesized in such a way that de-N-acetylated A-trisaccharide is attached to a polymer via the nitrogen in position C-2 of the galactosamine residue, in order to test the hypothesis that the supposed AB-epitope should be situated in the part of the molecule that is opposite to the NHAc group of GalNAc residue.Abstract:
Although the nature of the blood groups A and B has been comprehensively studied for a long time, it is still unclear as to what exactly is the epitope that is recognized by antibodies having AB specificity, i.e. monoclonal and polyclonal antibodies which are capable of interacting equally well with the antigens GalNAcalpha 1-3(Fucalpha 1-2)Gal (A trisaccharide) and Galalpha 1-3(Fucalpha 1-2)Gal (B trisaccharide), but do not react with their common fragment Fucalpha 1-2Gal. We have supposed that besides Fucalpha 1-2Gal, A and B antigens have one more shared epitope. The trisaccharides A and B are practically identical from the conformational point of view, the only difference being situated at position 2 of Galalpha residue, i.e. trisaccharide A has a NHAc group, whereas trisaccharide B has a hydroxyl group (see formulas). We have hypothesized that the AB-epitope should be situated in the part of the molecule that is opposite to the NHAc group of GalNAc residue. In order to test this hypothesis we have synthesized a polymeric conjugate in such a way that de-N-acetylated A-trisaccharide is attached to a polymer via the nitrogen in position C-2 of the galactosamine residue. In this conjugate the supposed AB-epitope should be maximally accessible for antibodies from the solution, whereas the discrimination site of antigens A and B by the antibodies should be maximally hidden due to the close proximity of the polymer. Interaction with several anti-AB monoclonal antibodies revealed that a part of them really interacted with the synthetic AB-glycotope, thus confirming our hypothesis. Moreover, similar antibodies were revealed in the blood of healthy blood group 0 donors. Analysis of spatial models was performed in addition to identify the hydroxyl groups of Fuc, Galalpha, and Galbeta residues, which are particularly involved in the composition of the AB-glycotope.read more
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The ABO blood group system revisited: a review and update.
Jill R. Storry,Martin L. Olsson +1 more
TL;DR: A review of key findings and recent progress made toward further understanding of this surprisingly polymorphic system of antigens of the ABO system is summarized.
Journal ArticleDOI
Anti-carbohydrate antibodies of normal sera: findings, surprises and challenges.
Margaret E. Huflejt,Marko Vuskovic,Daniela Vasiliu,Hongyu Xu,Polina Obukhova,Nadezhda Shilova,Alexander B. Tuzikov,Oxana Galanina,Banu K Arun,Karen H. Lu,Nicolai V. Bovin +10 more
TL;DR: The microchip format glycan array is used to characterize the individual carbohydrate recognition patterns by antibodies (Ab) in sera of 106 healthy donors and it is found that Ab are capable of recognizing the short inner core typical for glycolipids and glycoproteins (-GalNAcalpha) as a fragment of bigger glycans.
Journal ArticleDOI
Anti-carbohydrate antibodies elicited by polyvalent display on a viral scaffold.
Eiton Kaltgrad,Sayam Sen Gupta,Sreenivas Punna,Cheng-Yuan Huang,Aileen Y. Chang,Chi-Huey Wong,M. G. Finn,Ola Blixt +7 more
TL;DR: Comparable performance was observed for the virus‐based polyclonal versus a commercial monoclonal antibody raised against the globo‐H tetrasaccharide; this highlights the utility of the glycan microarray for both quality control and rapid in‐depth analysis.
Journal ArticleDOI
Glycopeptide dendrimers. Part II.
TL;DR: Glycopeptide dendrimers can be used as inhibitors of cell surface protein‐carbohydrate interactions, intervention with bacterial adhesion, for studying of recognition processes, diagnostics, imaging and contrast agents, mimetics, for complexation of different cationts, as site‐specific molecular delivery systems, for therapeutic purposes, as immunodiagnostics and in drug design.
Journal ArticleDOI
Identification of the sialic acid structures recognized by minute virus of mice and the role of binding affinity in virulence adaptation.
Hyun Joo Nam,Brittney Gurda-Whitaker,Wand Yee Gan,Shawen Ilaria,Robert McKenna,Padmaja Mehta,Richard Alvarez,Mavis Agbandje-McKenna +7 more
TL;DR: This study identifies the sialic acid structures recognized by MVM and provides rationale for the tropism of MVM for malignant transformed cells that contain sLex motifs and the neurotropism ofMVMi, which is likely mediated via interactions with multisialylated glycans known to be tumor cell markers.
References
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Journal ArticleDOI
Validation of the general purpose Tripos 5.2 force field
TL;DR: In this paper, a molecular mechanics force field implemented in the Sybyl program is described along with a statistical evaluation of its efficiency on a variety of compounds by analysis of internal coordinates and thermodynamic barriers.
Journal ArticleDOI
Synthesis of polymeric neoglycoconjugates based on N-substituted polyacrylamides.
Nicolai V. Bovin,Elena Korchagina,Tatyana V. Zemlyanukhina,N. E. Byramova,Oksana E. Galanina,Alexandr E. Zemlyakov,Alexandr E. Ivanov,Vitaly P. Zubov,L. V. Mochalova +8 more
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Haworth Memorial Lecture. Human blood groups and carbohydrate chemistry
Journal ArticleDOI
Computer simulation of histo-blood group oligosaccharides: energy maps of all constituting disaccharides and potential energy surfaces of 14 ABH and Lewis carbohydrate antigens.
TL;DR: Of special interest are the several different alignments that can be proposed for these molecules, which yield a realistic definition of the three-dimensional features of the epitopes thereby providing essential information about how carbohydrate antigens are recognized by proteins.
Journal Article
Genetic regulation of the expression of ABH and Lewis antigens in tissues.
TL;DR: Examples of these phenomena are presented in an effort to gain further insight into the genetic regulation of the expression of these complex oligosaccharide molecules.
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