Detection and Genetic Characterization of Deltacoronavirus in Pigs, Ohio, USA, 2014
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"Detection and Genetic Characterizat..." refers background or methods or result in this paper
...On the basis of 2 complete genome sequences from GenBank, of PorCoV HKU15-44 and HKU15-155, we designed 16 pairs of primers to determine the whole genome of extracted RNA samples (OH1987) from Farm 1 (Table 2)....
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...The OH1987 virus differs from HKU15-155 in the spike gene at nt 19469 and in the noncoding region at nt 25044; a 3-nt insertion is present at each location, making the whole-genome sequence of the OH1987 virus 6 nt longer than that of HKU15-155....
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...Phylogenetic analysis of the complete genome of PorCoV HKU15 OH1987 showed that the OH1987 strain clustered with the other 2 PorCoVs, HKU15-155 and HKU15-44, and was distinct from the bird deltacoronaviruses (Figure 1)....
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...Similar to the BLAST search results of partial N and M fragments, the BLAST search of the whole genome of the PorCoV HKU15 OH1987 showed 99% nt identity to PorCoV HKU15-155....
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...In addition, the phylogenetic trees constructed by using the amino acid sequences of the spike glycoprotein and nucleocapsid protein showed that the OH1987 virus clustered with HKU15-155 and HKU15-44 (Figure 2); this finding is in agreement with that in a previous study (1)....
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"Detection and Genetic Characterizat..." refers background in this paper
...Coronaviruses traditionally were classified into groups 1, 2, and 3 on the basis of their antigenic relationships (2)....
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"Detection and Genetic Characterizat..." refers background in this paper
...Virus from each coronavirus genus has been found in diverse host species, including mammals and birds (3)....
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...Viruses of the Alphacoronavirus, Betacoronavirus, and Deltacoronavirus genera have been detected in swine (3)....
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