Detection of SARS-CoV-2 and its S and N proteins using surface enhanced Raman spectroscopy
J.E. Sanchez,J.E. Sanchez,Sierra A. Jaramillo,Erik W. Settles,J. Jesus Velazquez Salazar,Alexander Lehr,Javier Gonzalez,Carmen Rodríguez Aranda,Hugo R. Navarro-Contreras,Marlee O. Raniere,Maxx Harvey,David M. Wagner,Andrew T. Koppisch,Robert S. Kellar,Paul Keim,Miguel Jose Yacaman +15 more
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TLDR
In this paper, a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained using surface enhanced Raman spectroscopy (SERS) of virion particles.Abstract:
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing for virus detection. This need has generated a high number of new testing methods aimed at replacing RT-PCR, which is the golden standard for testing. Most of the testing techniques are based on biochemistry methods and require chemicals that are often expensive and the supply might become scarce in a large crisis. In the present paper we suggest the use of methods based on physics that leverage novel nanomaterials. We demonstrate that using Surface Enhanced Raman Spectroscopy (SERS) of virion particles a very distinct spectroscopic signature of the SARS-CoV-2 virus can be obtained. We demonstrate that the spectra are mainly composed by signals from the spike (S) and nucleocapsid (N) proteins. It is believed that a clinical test using SERS can be developed. The test will be fast, inexpensive, and reliable. It is also clear that SERS can be used for analysis of structural changes on the S and N proteins. This will be an example of application of nanotechnology and properties of nanoparticles for health and social related matters.read more
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Surface-enhanced Raman scattering-based immunoassay for severe acute respiratory syndrome coronavirus 2
Hyun-Sook Cha,Hyeran Kim,Younju Joung,Jeong Moon,Hyowon Jang,So Yeon Park,Hyung-Jun Kwon,In-Chul Lee,Sunjoo Kim,Dongeun Yong,Sun-Woo Yoon,Sung-Gyu Park,Kyeonghye Guk,Eun Kyung Lim,Hyun Gyu Park,Jaebum Choo,Ju Yeon Jung,Taejoon Kang +17 more
TL;DR: A surface-enhanced Raman scattering (SERS)-based immunoassay involving an antibody pair, SERS-active hollow Au nanoparticles (NPs), and magnetic beads for the detection of SARS-CoV-2 was presented in this article .
Journal ArticleDOI
Ultra-sensitive and fast optical detection of the spike protein of the SARS-CoV-2 using AgNPs/SiNWs nanohybrid based sensors
Kais Daoudi,Kais Daoudi,Krithikadevi Ramachandran,Hussain Alawadhi,Rabah Boukherroub,Elhadj Dogheche,My Ali El Khakani,Mounir Gaidi +7 more
TL;DR: In this article, surface-enhanced Raman spectroscopy (SERS) was used to detect SARS-CoV-2 S1 (RBD) spike protein.
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Probing the mutation independent interaction of DNA probes with SARS-CoV-2 variants through a combination of surface-enhanced Raman scattering and machine learning
TL;DR: In this article , a set of DNA probes targeting a specific segment of the nucleocapsid phosphoprotein (N) gene of SARS-CoV-2 with high binding efficiency was developed.
Journal ArticleDOI
High-sensitivity and point-of-care detection of SARS-CoV-2 from nasal and throat swabs by magnetic SERS biosensor
TL;DR: In this article , a point-of-care detection method based on magnetic SERS biosensor composed of Fe3O4-Au nanocomposite and Au nanoneedles array was developed to detect SARS-CoV-2 directly.
Journal ArticleDOI
Plasmonic nanostructure-enhanced Raman scattering for detection of SARS-CoV-2 nucleocapsid protein and spike protein variants
TL;DR: In this paper , a surface-enhanced Raman scattering (SERS) response from the plasma-engineered, variant-specific antibody-functionalized silver nanoparticle-based nanoassemblies (AgMEN) interacting with the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins was demonstrated.
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