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Journal ArticleDOI

Determination of protein secondary structure by Fourier transform infrared spectroscopy: a critical assessment.

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This article is published in Biochemistry.The article was published on 1993-01-19. It has received 1460 citations till now. The article focuses on the topics: Fourier transform spectroscopy & Fourier transform infrared spectroscopy.

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Citations
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PatentDOI

Self-assembly and mineralization of peptide-amphiphile nanofibers

TL;DR: In this paper, pH-induced self-assembly of a peptide-amphiphile was used to make a nanostructured fibrous scaffold reminiscent of extracellular matrix.
Journal ArticleDOI

The Use and Misuse of FTIR Spectroscopy in the Determination of Protein Structure

TL;DR: This review critically assess the application of FTIR spectroscopy to the determination of protein structure by outlining the principles underlying protein secondary structure determination by FTIRSpectroscopy, and highlighting the situations in which FTIR Spectroscopy should be considered the technique of choice.
Journal ArticleDOI

What vibrations tell us about proteins

TL;DR: This review deals with current concepts of vibrational spectroscopy for the investigation of protein structure and function, namely the amide I vibration of the polypeptide backbone that is used for secondary-structure analysis and some of the general aspects also apply to RamanSpectroscopy.
Journal ArticleDOI

Instability, stabilization, and formulation of liquid protein pharmaceuticals.

TL;DR: The basic behavior of proteins, their instabilities, and stabilization in aqueous state in relation to the development of liquid protein pharmaceuticals is discussed.
Book ChapterDOI

[4] Circular dichroism

TL;DR: Circular dichroism is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left circularly polarized light and can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.
References
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Journal ArticleDOI

Examination of the secondary structure of proteins by deconvolved FTIR spectra.

TL;DR: Fourier transform ir (FTIR) spectra of 21 globular proteins have been obtained, revealing that the amide I band of each protein except casein consists of six to nine components, although all proteins do not exhibit components at every characteristic frequency.
Book ChapterDOI

Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins.

TL;DR: The aim of this chapter is to present recent developments in the vibrational spectroscopy of peptides, polypeptides, and proteins.
Journal ArticleDOI

Protein secondary structures in water from second-derivative amide I infrared spectra.

TL;DR: A method of analysis of second-derivative amide I spectra whereby the frequencies of bands due to different secondary structures can be obtained is demonstrated and the band intensities obtained provide a useful method for estimating the relative amounts of different structures.
Journal ArticleDOI

Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped Bands

TL;DR: In this article, the Fourier self-deconvolution (FDS) method was proposed to resolve overlapped lines that can not be instrumentally resolved due to their intrinsic linewidth.
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