Journal Article•
Development of a Lipid Particle for β-Carotene Encapsulation Using a Blend of Tristearin and Sunflower Oil: Choice of Lipid Matrix and Evaluation of Shelf Life of Dispersions
09 Sep 2013-Food Technology and Biotechnology (University of Zagreb, Faculty of Food Technology and Biotechnology (Sveuciliste u Zagrebu, Prehrambeno-Biotechnolosk i Fakultet))-Vol. 51, Iss: 3, pp 383-391
TL;DR: In this article, the authors choose a suitable lipid matrix to produce solid lipid particles, which would be used to encapsulate b-carotene, and evaluate the capacity of dispersions to protect the incorporated carotenoid.
Abstract: Summary Solid lipid particles are colloidal carriers that have been studied for almost 20 years in the pharmaceutical field and recently have been investigated by food researchers due to their capacity to enhance the incorporation of lipophilic bioactives and their bioavailability in aqueous formulations. The aims of this study are to choose a suitable lipid matrix to produce solid lipid particles, which would be used to encapsulate b-carotene, and to evaluate the capacity of dispersions to protect the incorporated carotenoid. Bulk lipid mixtures of tristearin and sunflower oil were analysed by differential scanning calorimetry and wide angle X-ray diffraction, and the mixture with the highest degree of structural disorganisation was chosen. b-Carotene was then encapsulated in solid lipid particles produced with this mixture, composed of 70 % tristearin and 30 % sunflower oil (6 % total lipid) and stabilised with hydrogenated soy lecithin and Tween 80 (3 % total surfactant) by hot pressure homogenisation. Two types of particles were produced, using one or two passages in the homogenisation step. Average particle size, zeta potential, thermal behaviour, crystallinity and b-carotene concentration were monitored over 4 months of storage (under refrigerated conditions). The results showed minor differences between the systems in terms of size distribution, although the particles produced with one passage through the homogeniser were slightly more efficient at protecting the b-carotene from degradation and also suffered few microstructural alterations after 4 months.
Citations
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TL;DR: In this paper, the authors evaluated the effects of solid lipid particles (SLP) incorporation on the aging process of NaCl-induced gels of soy protein isolate and locust bean gum.
Abstract: This study aimed to evaluate the effects of solid lipid particles (SLP) incorporation on the aging process of NaCl-induced gels of soy protein isolate and locust bean gum. The feasibility of incorporating beta-carotene (BC) into emulsion-filled gels (EFGs) was evaluated and the stability of the carotenoid was determined in two systems, SLP and NaCl-induced EFGs. Non-filled gels (NFG) and EFG were characterized to determine their microstructural organization, rheological properties and water holding capacity (WHC) throughout storage. In addition, the amount of BC contained in SLP and EFG was quantified for 30 days. SLP dispersions were stable and retained about 57% of the encapsulated bioactive carotenoid after 30 days. Given the good stability of the SLPs, they were considered suitable for incorporation into the gels and for the production of EFGs. Their incorporation increased the stability of the gel systems. The strength and WHC of NFG systems decreased with aging, while those of EFGs increased. EFGs provided greater protection of encapsulated beta-carotene (about 90% of retention after 30 days of storage) than SLPs, indicating that gelation of the continuous phase is a good strategy to prevent the degradation of encapsulated bioactive carotenoid.
2 citations
TL;DR: In this paper, the authors presented a simple, green and low cost analytical method for preconcentration and determination of β-carotene nanoparticle content in fruit and juice samples.
Abstract: This article presents a simple, green and low cost analytical method for preconcentration and determination of β-carotene nanoparticle content in fruit and juice samples. The method is based on the enrichment of β-carotene nanoparticles in a non-ionic surfactant phase using cloud point extraction (CPE) followed by UV-vis spectrophotometric determination at 468 nm. The influence of chemical variables such as pH of the sample solution, type and volume of the buffer, electrolyte type and concentration, surfactant concentration, temperature and incubation time on the CPE procedure was studied. Under optimum conditions the method yielded a linear calibration curve in the range of 2.80 × 10−8 to 2.52 × 10−6 mol L−1 for β-carotene nanoparticles (r = 0.9978). The LOD and LOQ were found to be 1.12 × 10−8 mol L−1 and 3.74 × 10−8 mol L−1, respectively. The relative standard deviations (RSD) for eight replicate measurements of 1.68 × 10−6 mg L−1 and 2.80 × 10−7 mol L−1 of BC-NPs were 3.2% and 5.6%, respectively. The developed CPE method was applied to the analysis of watermelon, tomato, cherry and orange juice samples. The recoveries of the analytes in spiked samples were in the range of 96–104%.
1 citations
05 Mar 2015
1 citations
TL;DR: In this article, the effects of lipid content and dilution on the properties and stability of nanostructured lipid carriers (NLCs) prepared from rambutan (Nephelium lappaceum L.) kernel fat were investigated.
Abstract: The effects of lipid content and dilution on the properties and stability of nanostructured lipid carriers (NLCs) prepared from rambutan (Nephelium lappaceum L.) kernel fat were investigated. The β...
1 citations
Patent•
15 Aug 2019
TL;DR: In this article, uses of lipid complexes with carotenoids for improvement properties of hydrocarbon-based products as well as related methods and uses are discussed. But the authors do not discuss the use of carotene in their work.
Abstract: The invention relates to uses of hydrocarbon / lipid complexes with carotenoids for improvement properties of hydrocarbon-based products as well as related methods and uses.
References
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TL;DR: This review summarizes relevant in vivo and in vitro methods used to assess the bioavailability of some nutrients, types of microstructural changes imparted by processing and during food ingestion that are relevant in matrix-nutrient interactions, and their effect on theBioavailability of selected nutrients.
Abstract: There is an increased interest in the role that some nutrients may play in preventing or ameliorating the effect of major diseases (for example, some types of cancer, cardiovascular diseases, eye disorders, among others). In this respect, the bioavailability or the proportion of an ingested nutrient that is made available (that is, delivered to the bloodstream) for its intended mode of action is more relevant than the total amount present in the original food. Disruption of the natural matrix or the microstructure created during processing may influence the release, transformation, and subsequent absorption of some nutrients in the digestive tract. Alternatively, extracts of bioactive molecules (for example, nutraceuticals) and beneficial microorganisms may be protected during their transit in the digestive system to the absorption sites by encapsulation in designed matrices. This review summarizes relevant in vivo and in vitro methods used to assess the bioavailability of some nutrients (mostly phytochemicals), types of microstructural changes imparted by processing and during food ingestion that are relevant in matrix-nutrient interactions, and their effect on the bioavailability of selected nutrients.
846 citations