Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm.
TL;DR: A rapid laboratory test kit was developed that is equally sensitive and reproducible as that described in the WHO protocol and can be used routinely in laboratories for assessment of plasma membrane integrity of sperm in vitro.
About: This article is published in Fertility and Sterility.The article was published on 2008-01-01. It has received 23 citations till now. The article focuses on the topics: Sperm plasma membrane & Sperm.
Citations
More filters
TL;DR: Subnormal sperm function in normospermia, asthenospermies, leukocytospermias, and leukocytes may be due to the cytotoxic effect of increased NO, whereas in oligospermIA the same may beDue to low or suboptimal levels.
49 citations
Cites methods from "Development of a rapid, sensitive, ..."
...A modified hypo-osmotic swelling test protocol developed in our laboratory as previously described (18) was adopted....
[...]
TL;DR: Reduction in test scores of sperm function, like HOS, AS, and NCD, in male partners of couples with idiopathic RPL suggests that sperms with altered or lowered functional competencies, if they fertilize the oocytes, may lead to the development of an unsustainable embryo resulting in early pregnancy loss.
44 citations
Cites methods from "Development of a rapid, sensitive, ..."
...A modified hypo-osmotic swelling (HOS) test protocol was adopted as developed in our laboratory and described (12)....
[...]
TL;DR: In couples treated with IUI, the clinical validity of SCSA is higher than that of sperm morphology alone, but not enough to introduce SCSA as a test in male infertility work-up.
Abstract: The present study is based on a PubMed search and compares the clinical validity of classical semen parameters (CSP) and the sperm chromatin structure assay (SCSA) in different clinical contexts. The PubMed database was searched using keywords on the sperm diagnostic test for pregnancy in three clinical scenarios: (i) couples attempting to conceive; (ii) couples who had been attempting to conceive for 12months without success; and (iii) couples treated with intrauterine insemination (IUI). There was a considerable heterogeneity among the studies included. For couples attempting to conceive following a SCSA that produced an abnormal result, the likelihood of male factor infertility ranged from a pre-test value of 7.5% to a post-test value of 32.1% [95% confidence interval (CI) 15.7-54.5], while after CSP with an abnormal result, the post-test probability was 17.3% (95% CI 11.8-24.5). For a pre-test prevalence of male factor infertility of 50%, the post-test probability of male factor infertility after an abnormal test is very similar for both SCSA and CSP. In couples treated with IUI, the clinical validity of SCSA is higher than that of sperm morphology alone, but not enough to introduce SCSA as a test in male infertility work-up.
42 citations
Cites background or methods from "Development of a rapid, sensitive, ..."
...…couples who are to receive IUI treatment for unexplained infertility, it is important to compare its clinical validity with that of other, more economical sperm function tests, such as the hypo-osmotic swelling test (Check, 2005; Misro and Chaki, 2008) or 24-h survival test (Branigan et al., 1999)....
[...]
...Before recommending the use of SCSA for couples who are to receive IUI treatment for unexplained infertility, it is important to compare its clinical validity with that of other, more economical sperm function tests, such as the hypo-osmotic swelling test (Check, 2005; Misro and Chaki, 2008) or 24-h survival test (Branigan et al....
[...]
TL;DR: HOS-test and vital stain cannot be used interchangeably in all circumstances for sperm viability determination, but the HOS- test efficiency can be improved by assessing SDTS in the sample and incorporating SDTS as a correction factor.
Abstract: Purpose
To investigate the prevalence of spontaneously developed tail swellings (SDTS) in human sperm samples that are commonly encountered in the laboratory, and their influence on the hypo-osmotic swelling test (HOS-test).
32 citations
Cites background from "Development of a rapid, sensitive, ..."
...This may be due to the freeze-thaw related impact on sperm tails [8, 10, 17]....
[...]
TL;DR: Aqueous stem bark extract of the plant showed dose dependent loss of sperm motility by influencing the morphological deformation, blockage in nuclear envelope and distinct declination in acrosomal status of spermatozoa.
25 citations
References
More filters
2,119 citations
TL;DR: The study suggests that the current WHO guidelines for normal semen quality should be used with caution, as some men with sperm counts above the lower limit of the normal range defined by WHO may in fact be subfertile.
703 citations
TL;DR: A range of in vitro tests have been developed to monitor various aspects of sperm function including their potential for movement, cervical mucus penetration, capacitation, zona recognition, the acrosome reaction and sperm-oocyte fusion and such functional assays have been found to predict the fertilizing capacity of human spermatozoa in vitro and in vivo with some accuracy.
Abstract: Traditionally, the diagnosis of male infertility has depended upon a descriptive evaluation of human semen with emphasis on the number of spermatozoa that are present in the ejaculate, their motility and their morphology. The fundamental tenet underlying this approach is that male fertility can be defined by reference to a threshold concentration of motile, morphologically normal spermatozoa that must be exceeded in order to achieve conception. Many independent studies have demonstrated that this fundamental concept is flawed and, in reality, it is not so much the absolute number of spermatozoa that determines fertility, but their functional competence. In the light of this conclusion, a range of in vitro tests have been developed to monitor various aspects of sperm function including their potential for movement, cervical mucus penetration, capacitation, zona recognition, the acrosome reaction and sperm-oocyte fusion. Such functional assays have been found to predict the fertilizing capacity of human spermatozoa in vitro and in vivo with some accuracy. Recent developments in this field include the introduction of tests to assess the degree to which human spermatozoa have suffered oxidative stress as well as the integrity of their nuclear and mitochondrial DNA. Such assessments not only yield information on the fertilizing capacity of human spermatozoa but also their ability to support normal embryonic development.
286 citations
TL;DR: This review is an attempt to clarify and update the usefulness of the HOS test as a tool to evaluate the sperm function.
Abstract: The hypoosmotic swelling (HOS) test is a relatively new assay used to evaluate the functional integrity of the sperm's plasma membrane. In fact, more studies have been published on the applicability of the HOS test than any other new sperm indicator. The assay is based on the fact that fluid transport occurs across an intact cell membrane under hypoosmotic conditions until equilibrium is reached. Due to the influx of fluid, the cell will expand and bulge, especially in the tail, and this change can be readily observed with a phase contrast microscope. Earlier studies have yielded some confusion regarding the interpretation of the data. This review is an attempt to clarify and update the usefulness of the HOS test as a tool to evaluate the sperm function.
190 citations
TL;DR: It has been found that spermatozoa of bull, rabbit and man swell in hypotonic media and in media of penetrating non-electrolytes and coiled spermatoza in ejaculates apparently are the result of osmotic swelling.
182 citations