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Journal ArticleDOI

Development of bioanalytical parameters for standardization of Azadiracta indica

01 Jun 2013-Asian Pacific Journal of Reproduction (No longer published by Elsevier)-Vol. 2, Iss: 2, pp 132-135
TL;DR: In this article, preliminary phytochemical analysis, total phenol, flavonoid, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis through HPTLC method were performed.
About: This article is published in Asian Pacific Journal of Reproduction.The article was published on 2013-06-01 and is currently open access. It has received None citations till now. The article focuses on the topics: Rutin.
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Journal Article
TL;DR: In this paper, the use of Folin-Ciocalteu reagent rather than the FolinDenis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period was investigated.
Abstract: Several details of the assay of total phenolic substances have been investigated and an improved procedure developed. The improvements include the use of Folin-Ciocalteu reagent rather than the Folin-Denis reagent, gallic acid as a reference standard, and a more reproducible time-temperature color development period. The values obtained are less subject to variation and interference from several nonphenols, yet are directly comparable to the "tannin" values obtained by the previously standard method.

18,629 citations

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TL;DR: In this article, two complementary colorimetric methods, aluminum chloride method and 2,4-dini trophenylhydrazine method, were used to determine the real content of total flavonoids in propolis.

3,899 citations

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TL;DR: The information provided in this review will help the researchers for the development of an alternative methods rather than insulin and oral hypoglycemic agents for the treatment of diabetes mellitus, which will minimize the complication associated with the diabetes and related disorder.
Abstract: Diabetes mellitus is not a single disease but is a group of metabolic disorders affecting a huge number of population in the world. It is mainly characterized by chronic hyperglycemia, resulting from defects in insulin secretion or insulin action. It is predicated that the number of diabetes person in the world could reach upto 366 million by the year 2030. Even though the cases of diabetes are increasing day by day, except insulin and oral hypoglycemic drugs no other way of treatment has been successfully developed so far. Thus, the objective of the present review is to provide an insight over the pathophysiological and etiological aspects of diabetes mellitus along with the remedies available for this metabolic disorder. The review also contains brief idea about diabetes mellitus and the experimental screening model with their relevant mechanism and significance mainly used nowadays. Alloxan and streptozotocin are mainly used for evaluating the antidiabetic activity of a particular drug. This review contain list of medicinal plants which have been tested for their antidiabetic activity in the alloxan induced diabetic rat model. From the available data in the literature, it was found that plant having antidiabetic activity is mainly due to the presence of the secondary metabolite. Thus, the information provided in this review will help the researchers for the development of an alternative methods rather than insulin and oral hypoglycemic agents for the treatment of diabetes mellitus, which will minimize the complication associated with the diabetes and related disorder.

268 citations

Journal ArticleDOI
TL;DR: Bark extract is more effective than melatonin, vitamin E, desferrioxamine and alpha-phenyl N-tert butylnitrone, the known antioxidants having antiulcer effect and well tolerated by rats with no significant adverse effect.

147 citations

Journal Article
TL;DR: In this article, three samples of Carica papaya leaves (Green, Yellow and Brown) were collected randomly from Ogbomoso town, Oyo state, Nigeria and analyzed for the phytochemical composition, vitamins and mineral constituents.
Abstract: Three samples of Carica papaya leaves (Green, Yellow and Brown) were collected randomly from Ogbomoso town, Oyo state, Nigeria and analyzed for the phytochemical composition, vitamins and mineral constituents. Phytochemical screening revealed the presence of bioactive compound saponins, cardiac glycoside alkaloids and absence of tannins in the three samples. Results showed that the plant leaves contained the vitamins, (mg/100g), thiamine (B1): green leaves 0.94, yellow leaves 0.41, brown leaves 0.52; riboflavin (B2): green leaves 0.13, yellow leaves 0.04, brown leaves 0.06; ascorbic acid (C): green leaves 16.29, yellow green 9.62, brown leaves 11.26. Mineral analysis showed highest values (mg/kg) of Ca, 8612.50; Mg, 67.75; Na, 1782.00; K, 2889.00; Mn,9.50 in the green leaves, and Fe, 147.50 in yellow leaves as compared to other elements examined. Thus green pawpaw leaf gave a source of essential nutrients while yellow pawpaw was a source of iron. Therefore pawpaw leaves can be manipulated in the herbal treatment of various diseases and as a potential source of useful elements for drugs formulation.

141 citations