Development of procedures for sreening for, identification and/or validated quantification of herbal drugs in blood or urine using GC-MS, LC-MS or LC-MS/MS
01 Jan 2006-
About: The article was published on 2006-01-01 and is currently open access. It has received 1 citations till now.
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Journal Article•
TL;DR: In this paper, a method was proposed for the determination of nicotine and cotinine in human urine, plasma and saliva, and the results showed that through the accurate determination of cotinines in saliva, the risk of ETS-exposed human can be predicted.
104 citations
References
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TL;DR: Practical, experimental approaches for studying, identifying, and eliminating the effect of matrix on the results of quantitative analyses by HPLC-MS/MS are described and it is demonstrated that, for the investigational drug under study, the matrix effect was clearly observed when ISP interface was utilized but it was absent when the HN interface was employed.
Abstract: In recent years, high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS/MS) detection has been demonstrated to be a powerful technique for the quantitative determination of drugs and metabolites in biological fluids. However, the common and early perception that utilization of HPLC−MS/MS practically guarantees selectivity is being challenged by a number of reported examples of lack of selectivity due to ion suppression or enhancement caused by the sample matrix and interferences from metabolites. In light of these serious method liabilities, questions about how to develop and validate reliable HPLC−MS/MS methods, especially for supporting long-term human pharmacokinetic studies, are being raised. The central issue is what experiments, in addition to the validation data usually provided for the conventional bioanalytical methods, need to be conducted to confirm HPLC−MS/MS assay selectivity and reliability. The current regulatory requirements include the need for the assessment and...
4,543 citations
Book•
01 Jan 1966
TL;DR: In this paper, the basic mechanism of ion fragmentation is described and a theory of unimolecular Ion Decompositions is proposed, along with a detailed model of the mass spectrum of common compound classes.
Abstract: 1. Introduction 2. Elemental Composition 3. The Molecular Ion 4. Basic Mechanisms of Ion Fragmentation 5. Postulation of Molecular Structures 6. Auxiliary Techniques 7. Theory of Unimolecular Ion Decompositions 8. Detailed Mechanisms of Ion Fragmentation 9. Mass Spectra of Common Compound Classes 10.Computer Identification of Unknown Mass Spectra 11.Solutions to Unknowns Bibliography Appendix Index
2,069 citations
TL;DR: In this article, a simple relation exists between the retention index of a compound on a non-polar stationary phase and its boiling point, which is defined as the ratio of the boiling point of the compound to its retention index.
Abstract: For the characterization of organic substances in gas chromatography a number termed «retention index» is proposed. A simple relation exists between the retention index of a compound on a non-polar stationary phase and its boiling point.
1,916 citations
TL;DR: The purpose of this report is to represent the progress in analytical methodologies over the last decade and assessment of the major agreements and issues discussed with regard to small molecules at both the conference and the workshop.
Abstract: This report is a synthesis of (1) the earlier conference on Analytical Methods Validation−Bioavailability, Bioequivalence and Pharmacokinetic Studies (Conference held in Arlington, VA, December 3–5, 1990 and the report published in Pharmaceutical Research, 9: 588-592, 1992) and (2) the workshop on “Bioanalytical Methods Validation—A Revisit with a Decade of Progress,” (Workshop held in Arlington, VA, January 12–14, 2000), sponsored by the American Association of Pharmaceutical Scientists and the U. S. Food and Drug Administration. The bioanalytical method validation workshop of January 12–14, 2000 was directed towards small molecules. A separate workshop was held in March 1–3, 2000 to discuss validation principles for macromolecules. The purpose of this report is to represent the progress in analytical methodologies over the last decade and assessment of the major agreements and issues discussed with regard to small molecules at both the conference and the workshop. The report is also intended to provide guiding principles for validation of bioanalytical methods employed in support of bioavailability, bioequivalence, and pharmacokinetic studies in man and in animals.
1,588 citations
TL;DR: Important considerations in analytical method validation will be discussed and may be used as guidance by scientists wishing to develop and validate analytical methods.
1,157 citations