Development of procedures for sreening for, identification and/or validated quantification of herbal drugs in blood or urine using GC-MS, LC-MS or LC-MS/MS
01 Jan 2006-
About: The article was published on 2006-01-01 and is currently open access. It has received 1 citations till now.
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Journal Article•
TL;DR: In this paper, a method was proposed for the determination of nicotine and cotinine in human urine, plasma and saliva, and the results showed that through the accurate determination of cotinines in saliva, the risk of ETS-exposed human can be predicted.
104 citations
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TL;DR: A gas chromatographic-mass spectrometric procedure for the quantitation of buprenorphine and norbuprenorphines has been developed in which the analytes were converted, after enzyme hydrolysis, to their methyl derivatives by direct extractive alkylation using tetrahexylammonium hydrogen sulphate phase transfer reagent and iodomethane dissolved in tert.-butylmethyl ether.
46 citations
TL;DR: This method proved to be robust and accurate for the quantification of nicotine, cotinine, trans-3'-hydroxy Cotinine, and norcotinine in human plasma collected in clinical studies of acute nicotine effects on brain activity and on the development of neonates of maternal smokers.
Abstract: A liquid chromatographic-mass spectrometric method for the simultaneous determination of nicotine, cotinine, trans-3′-hydroxycotinine, and norcotinine in human plasma was developed and validated. Analytes and deuterated internal standards were extracted from human plasma using solid-phase extraction and analyzed by liquid chromatography/atmospheric pressure chemical ionization-mass spectrometric detection with selected ion monitoring (SIM). Limits of detection and quantification were 1.0 and 2.5 ng/ml, respectively, for all analytes. Linearity ranged from 2.5 to 500 ng/ml of human plasma using a weighting factor of 1/x; correlation coefficients for the calibration curves were > 0.99. Intra- and inter-assay precision and accuracy were < 15.0%. Recoveries were 108.2–110.8% nicotine, 95.8–108.7% cotinine, 90.5–99.5% trans-3′-hydroxycotinine, and 99.5–109.5% norcotinine. The method was also partially validated in bovine serum, owing to the difficulty of obtaining nicotine-free human plasma for the preparation of calibrators and quality control (QC) samples. This method proved to be robust and accurate for the quantification of nicotine, cotinine, trans-3′-hydroxycotinine, and norcotinine in human plasma collected in clinical studies of acute nicotine effects on brain activity and on the development of neonates of maternal smokers. Copyright © 2006 John Wiley & Sons, Ltd.
43 citations
TL;DR: A highly sensitive and selective quantitative LC-ESI-MS-MS method for the detection of colchicine in sheep serum and milk was developed and validated and demonstrated in serum and the corresponding milk samples from Albanian sheep showed clinical signs of intoxication.
Abstract: Colchicine is a naturally occurring alkaloid used in human and veterinary medicine. It shows genotoxicity in in vitro and in vivo systems even at low concentrations. Therefore, no ADI has been established, and colchicine has been included in Annex IV of Council Regulation (EEC) No. 2377/90. No abuse of this drug in intensive livestock farming has yet been reported. However, there may be a natural route of entry for this compound into the food chain when Colchicum autumnale is consumed by animals kept outdoors. To address this concern, we developed and validated a highly sensitive and selective quantitative LC-ESI-MS-MS method for the detection of colchicine in sheep serum and milk. For sample pretreatment, all samples were liquid−liquid extracted with phosphate buffer (pH 8.0) and dichloromethane. LC separation was carried out on an RP C18 column employing a 0.5% formic acid/acetonitrile gradient system. The recoveries in both matrixes at a concentration range from 0.0005 to 1 mg/L were >80% with RSDs of ...
42 citations
TL;DR: A method was developed for the efficient determination of nicotine and cotinine in rat plasma samples originating from nicotine exposure studies and the repeatability ranged from 5 to 12% (R.S.D.) for nicotine and from 3 to 5% for cotinines at the concentration level of 1-60 microg/l (n = 4).
40 citations
TL;DR: The procedure is an improvement over previous extractive alkylation methods because of the development of a simple clean-up step using a macroreticular acrylic copolymer to remove the coextracted phase-transfer reagent from the organic phase after derivatization.
39 citations