Development of spike receptor-binding domain nanoparticle as a vaccine candidate against SARS-CoV-2 infection in ferrets
Young-Il Kim,Dokyun Kim,Kwang-Min Yu,Hogyu David Seo,Shin-Ae Lee,Mark Anthony B. Casel,Seung-Gyu Jang,Stephanie Kim,WooRam Jung,Chih-Jen Lai,Young Ki Choi,Jae U. Jung +11 more
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TLDR
In this paper, the Spike RBD-nanoparticle was used as an effective protein vaccine candidate against SARS-CoV-2 infection in 16-20 months old ferrets by intramuscular or intranasal inoculation.Abstract:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a causative agent of COVID-19 pandemic, enters host cells via the interaction of its Receptor-Binding Domain (RBD) of Spike protein with host Angiotensin-Converting Enzyme 2 (ACE2). Therefore, RBD is a promising vaccine target to induce protective immunity against SARS-CoV-2 infection. In this study, we report the development of RBD protein-based vaccine candidate against SARS-CoV-2 using self-assembling H. pylori -bullfrog ferritin nanoparticles as an antigen delivery. RBD-ferritin protein purified from mammalian cells efficiently assembled into 24-mer nanoparticles. 16-20 months-old ferrets were vaccinated with RBD-ferritin nanoparticles (RBD-nanoparticles) by intramuscular or intranasal inoculation. All vaccinated ferrets with RBD-nanoparticles produced potent neutralizing antibodies against SARS-CoV-2. Strikingly, vaccinated ferrets demonstrated efficient protection from SARS-CoV-2 challenge, showing no fever, body weight loss and clinical symptoms. Furthermore, vaccinated ferrets showed rapid clearance of infectious viruses in nasal washes and lungs as well as viral RNA in respiratory organs. This study demonstrates the Spike RBD-nanoparticle as an effective protein vaccine candidate against SARS-CoV-2.read more
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Mucosal Vaccines, Sterilizing Immunity, and the Future of SARS-CoV-2 Virulence
TL;DR: The pros and cons of each vaccination strategy, the current mucosal SARS-CoV-2 vaccines under development, and their implications for public health are reviewed.
Journal ArticleDOI
Molecular Aspects concerning the Use of the SARS-CoV-2 Receptor Binding Domain as a Target for Preventive Vaccines
Yury Valdés-Balbín,Darielys Santana-Mederos,Françoise Paquet,Sonsire Fernández,Yanet Climent,Fabrizio Chiodo,Fabrizio Chiodo,Laura Rodriguez,Belinda Sánchez Ramírez,Kalet León,Tays Hernández,Lila Castellanos-Serra,Raine Garrido,Guang-Wu Chen,Dagmar García-Rivera,Daniel G. Rivera,Vicente Verez-Bencomo +16 more
TL;DR: In this paper, the authors summarized the molecular aspects associated with the interaction of SARS-CoV-2 RBD with its receptor in human cells, the angiotensin-converting enzyme 2 (ACE2), the epitopes involved in the neutralizing activity, and the impact of virus mutations thereof.
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A novel and highly divergent Canine Distemper Virus lineage causing distemper in ferrets in Australia
Ankita M. George,Michelle Wille,Michelle Wille,Jianning Wang,Keith Anderson,Shari Cohen,Jean M. Moselen,Leo Yi Yang Lee,Willy W. Suen,John Bingham,Antonia E Dalziel,Aeron C. Hurt,David T. Williams,Yi-Mo Deng,Ian G. Barr +14 more
TL;DR: In this article, the authors reported an outbreak of distemper in ferrets in two independent research facilities in Australia and revealed that the outbreak at both facilities was caused by a single, novel lineage of CDV, which was highly divergent across the H gene, F signal peptide and full genome.
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Nanovaccines to combat virus-related diseases.
TL;DR: Given the safety and extensive application of subunit vaccines, and the rapid rise of mRNA vaccines, this review mainly focuses on these two vaccine strategies and provides an overview of the nanoparticle-based vaccine delivery platforms to tackle the current and next global health challenges.
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