Developmental changes in human megakaryopoiesis
Olivier Bluteau,Thierry Langlois,Thierry Langlois,Paola Rivera-Munoz,Paola Rivera-Munoz,F. Favale,F. Favale,Philippe Rameau,Guillaume Meurice,Philippe Dessen,Philippe Dessen,Eric Solary,Eric Solary,Hana Raslova,Hana Raslova,Thomas Mercher,Thomas Mercher,Najet Debili,Najet Debili,William Vainchenker,William Vainchenker +20 more
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TLDR
The molecular bases of the cellular changes that occur during human megakaryocyte (MK) ontogeny remain unknown, and may be important for understanding the significance of MK differentiation from human embryonic stem cells (hESCs).About:
This article is published in Journal of Thrombosis and Haemostasis.The article was published on 2013-09-01 and is currently open access. It has received 68 citations till now. The article focuses on the topics: Cellular differentiation.read more
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Journal ArticleDOI
Transcriptional diversity during lineage commitment of human blood progenitors
Lu Chen,Lu Chen,Lu Chen,Myrto Kostadima,Myrto Kostadima,Myrto Kostadima,Joost H.A. Martens,Giovanni Canu,Giovanni Canu,Sara P. Garcia,Sara P. Garcia,Ernest Turro,Ernest Turro,Kate Downes,Kate Downes,Iain C. Macaulay,Ewa Bielczyk-Maczyńska,Ewa Bielczyk-Maczyńska,Sophia Coe,Sophia Coe,Samantha Farrow,Samantha Farrow,Pawan Poudel,Pawan Poudel,Frances Burden,Frances Burden,Sjoert B. G. Jansen,Sjoert B. G. Jansen,William J. Astle,William J. Astle,Antony P. Attwood,Antony P. Attwood,Tadbir K. Bariana,Bernard de Bono,Bernard de Bono,Alessandra Breschi,John C. Chambers,John C. Chambers,Fizzah A. Choudry,Fizzah A. Choudry,Laura Clarke,Paul Coupland,Martijn van der Ent,Wendy N. Erber,Joop H. Jansen,Rémi Favier,Matthew Fenech,Nicola Foad,Nicola Foad,Kathleen Freson,Chris Van Geet,Keith Gomez,Roderic Guigó,Daniel Hampshire,Daniel Hampshire,Anne M. Kelly,Anne M. Kelly,Anne M. Kelly,Hindrik H. D. Kerstens,Jaspal S. Kooner,Jaspal S. Kooner,Michael Laffan,Claire Lentaigne,Charlotte Labalette,Charlotte Labalette,Tiphaine Martin,Tiphaine Martin,Tiphaine Martin,Stuart Meacham,Stuart Meacham,Andrew D Mumford,Sylvia T. Nurnberg,Sylvia T. Nurnberg,Emilio Palumbo,Bert A. van der Reijden,David J. Richardson,Stephen John Sammut,Greg Slodkowicz,Asif U. Tamuri,Louella Vasquez,Katrin Voss,Katrin Voss,Stephen Watt,Sarah K Westbury,Paul Flicek,Paul Flicek,Remco Loos,Nick Goldman,Paul Bertone,Paul Bertone,Randy J. Read,Sylvia Richardson,Ana Cvejic,Ana Cvejic,Nicole Soranzo,Nicole Soranzo,Willem H. Ouwehand,Willem H. Ouwehand,Willem H. Ouwehand,Hendrik G. Stunnenberg,Mattia Frontini,Mattia Frontini,Augusto Rendon,Augusto Rendon +103 more
TL;DR: A previously undetected layer of regulation affecting cell fating is unveiled, which involves transcriptional isoforms switching without noticeable changes at the gene level and resulting in the gain or loss of protein functions in blood cells involved in the immune system.
Journal ArticleDOI
Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming
Thomas Moreau,Amanda L. Evans,Amanda L. Evans,Louella Vasquez,Marloes R. Tijssen,Ying Yan,Matthew Trotter,Daniel Howard,Daniel Howard,Maria Colzani,Maria Colzani,Meera Arumugam,Meera Arumugam,Wing Han Wu,Wing Han Wu,Amanda Dalby,Amanda Dalby,Riina Lampela,Guenaelle Bouet,Guenaelle Bouet,Catherine M. Hobbs,Catherine M. Hobbs,Dean C. Pask,Dean C. Pask,Holly Payne,Tatyana Ponomaryov,Alexander Brill,Nicole Soranzo,Willem H. Ouwehand,Roger A. Pedersen,Roger A. Pedersen,Cedric Ghevaert,Cedric Ghevaert +32 more
TL;DR: The high cell purity and yield achieved by MK forward programming, combined with efficient cryopreservation and good manufacturing practice (GMP)-compatible culture, make this approach eminently suitable to both in vitro production of platelets for transfusion and basic research in MK and platelet biology.
Supplementary resources_Transcriptional diversity during lineage commitment of human blood progenitors
Lu Chen,Myrto Kostadima,Joost H.A. Martens,Giovanni Canu,Sara P. Garcia,Ernest Turro,Kate Downes,Iain C. Macaulay,E. Bielczyk Maczynska,S. Coe,Samantha Farrow,Pawan Poudel,Frances Burden,Sjoert B. G. Jansen,William J. Astle,Antony P. Attwood,Tadbir K. Bariana,B. de Bono,Alessandra Breschi,John C. Chambers,Fizzah A. Choudry,Laura Clarke,Paul Coupland,M. van der Ent,Wendy N. Erber,Joop H. Jansen,Rémi Favier,Matthew Fenech,Nicola Foad,Kathleen Freson,C. van Geet,Keith Gomez,Roderic Guigó,D. Hampshire,Anne M. Kelly,Hindrik H. D. Kerstens,Jaspal S. Kooner,Michael Laffan,Claire Lentaigne,Charlotte Labalette,Tiphaine Martin,Stuart Meacham,Andrew D Mumford,Sylvia T. Nurnberg,Emilio Palumbo,B. A. van der Reijden,David J. Richardson,Stephen John Sammut,Greg Slodkowicz,Asif U. Tamuri,Louella Vasquez,Katrin Voss,Stephen Watt,Sarah K Westbury,Paul Flicek,Remco Loos,Nick Goldman,P. Bertone,Randy J. Read,Sylvia Richardson,Ana Cvejic,Nicole Soranzo,Willem H. Ouwehand,Hendrik G. Stunnenberg,Mattia Frontini,Augusto Rendon +65 more
Journal ArticleDOI
Understanding platelet generation from megakaryocytes: implications for in vitro–derived platelets
Xiuli Sim,Xiuli Sim,Mortimer Poncz,Mortimer Poncz,Paul Gadue,Paul Gadue,Deborah L. French,Deborah L. French +7 more
TL;DR: The understanding of committed megakaryocytes and platelet release in vivo and in vitro, and how this knowledge can guide the development of in vitro-derived platelets for clinical application are focused on.
Journal ArticleDOI
The convergent roles of the nuclear factor I transcription factors in development and cancer
TL;DR: The evidence suggesting a converging role for the NFI genes in development and cancer is summarized, and the challenges impeding the understanding of NFI function in cancer biology are presented, to demonstrate how a developmental perspective may contribute towards overcoming such hurdles.
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