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Journal ArticleDOI

Developmental changes of the glycolytic enzymes in the human fetal heart

01 Dec 1985-Journal of Biosciences (Springer India)-Vol. 9, Iss: 3, pp 159-163
TL;DR: Though considerable activity for aldolase is observed at an early period, it declines thereafter, but again increases in the later period, and the probable role and correlations of these glycolytic enzymes with energy demand and general functional development in human fetal heart in ontogeny are evaluated.
Abstract: The ontogeny of hexokinase, phosphofructokinase, phosphoglucoisornerase, aldolase, pyruvate kinase and lactate dehydrogenase activities which are associated with glycolysis, an important energy yielding process, has been studied in human fetal heart for periods ranging from 13 weeks to above 33 weeks of gestation. Hexokinase, phosphoglucoisomerase and pyruvate kinase activities show similar developmental profiles exhibiting maximum activity at 25–28 weeks ofgestation. Phosphofructokinase activity, on the other hand, shows a minimum at this period and exhibits a peak value at early stages (13–16 weeks of gestation). Though considerable activity for aldolase is observed at an early period, it declines thereafter, but again increases in the later period. The probable role and correlations of these glycolytic enzymes with energy demand and general functional development in human fetal heart in ontogeny are evaluated.
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Journal Article
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.

289,852 citations


"Developmental changes of the glycol..." refers methods in this paper

  • ...Protein content of the samples was determined by the method of Lowry et al. (1951)....

    [...]

Journal ArticleDOI
TL;DR: Fatty acid oxidation is suppressed in ischemic hearts leading to accumulation of long-chained CoA derivatives and increase in triglyceride levels, and the acceleration of flux through glycolysis may be as much as 10to 20-fold.
Abstract: Utilization of carbohydrate and fatty acids is strongly influenced by rates of con­ sumption and production of high energy compounds in cardiac muscle. In well­ oxygenated hearts, fatty acid has been identified as the preferred substrate by both in vivo and in vitro studies (for review see 146). Availability of fatty acid suppresses glucose utilization by inhibition of several steps in the glycolytic pathway. Since a variety of longand short-chained fatty substrates are inhibitory and since the effect is abolished in hearts perfused under anaerobic conditions, oxidation of the fatty acid appears to be required for this effect. When energy utilization of well­ oxygenated hearts is increased, consumption of fatty acids and/or glucose is ac­ celerated. Under these conditions, fatty acids remain the preferred oxidative substrate. Availability of oxygen to the heart can be restricted either by lowering or reducing to zero the oxygen tension of the perfusate, thereby inducing hypoxia or anoxia, or by restricting the flow of perfusate containing high oxygen tensions to induce ischemia. In hypoxic or anoxic hearts, fatty acid oxidation is suppressed and glycol­ ysis is stimulated. The acceleration of flux through glycolysis may be as much as 10to 20-fold. On the other hand, ischemia results in only a transient increase in glycolytic flux that is followed within 3-4 min by inhibition. Fatty acid oxidation is suppressed in ischemic hearts leading to accumulation of long-chained CoA derivatives and increase in triglyceride levels.

1,270 citations


"Developmental changes of the glycol..." refers background in this paper

  • ...Neely and Morgan (1974) showed that a decrease in fructose-6-phosphate results in an increase in fructose- 1,6-diphosphate associated with faster glycolytic flow....

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Journal ArticleDOI

868 citations


"Developmental changes of the glycol..." refers methods in this paper

  • ...Phosphoglucoisomerase was assayed by the method of Roe et al. (1949) and pyruvate kinase was assayed by the method of Weber et al. (1965)....

    [...]

Journal ArticleDOI
02 Jul 1965-Science
TL;DR: Evidence indicated that the increased pyruvate kinase activity induced by inisulin entails at a certain stage a stimulation of the synthesis of certain RNA species relevant to the production of this enzyme.
Abstract: The hepatic pyruvate kinase activity markedly decreased when rats were made diabetic by alloxan injection. Insulin treatment induced new synthesis of pyruvate kinase which was prevented by injection of ethionine and actinomycin D. The evidence indicated that the increased pyruvate kinase activity induced by inisulin entails at a certain stage a stimulation of the synthesis of certain RNA species relevant to the production of this enzyme.

130 citations


"Developmental changes of the glycol..." refers methods in this paper

  • ...Phosphoglucoisomerase was assayed by the method of Roe et al. (1949) and pyruvate kinase was assayed by the method of Weber et al. (1965)....

    [...]

Journal ArticleDOI

123 citations


"Developmental changes of the glycol..." refers background in this paper

  • ...Besides, it has been well established that the inhibition of phosphofructokinase leads to the accumulation of glucose-6-phosphate which in turn inhibits the activity of hexokinase (Weil-Malherbe and Bone, 1951)....

    [...]