Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes.
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TLDR
In this paper, it was shown that around gestational week 18, EGFR-expressing human cortical truncated radial glial cells (tRGs) give rise to basal multipotent intermediate progenitors (bMIPCs) that express EGFR, ASCL1, OLIG2 and OLIG1.Abstract:
Human cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons, glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interneurons. One of prominent features of the human cortex is enriched with glial cells, but there are major gaps in understanding how these glial cells are generated. Herein, by integrating analysis of published human cortical single-cell RNA-Seq datasets with our immunohistochemistical analyses, we show that around gestational week 18, EGFR-expressing human cortical truncated radial glial cells (tRGs) give rise to basal multipotent intermediate progenitors (bMIPCs) that express EGFR, ASCL1, OLIG2 and OLIG1. These bMIPCs undergo several rounds of mitosis and generate cortical oligodendrocytes, astrocytes and olfactory bulb interneurons. We also characterized molecular features of the cortical tRG. Integration of our findings suggests a general picture of the lineage progression of cortical radial glial cells, a fundamental process of the developing human cerebral cortex.read more
Citations
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References
More filters
Journal ArticleDOI
Comprehensive Integration of Single-Cell Data.
Tim Stuart,Andrew Butler,Paul J. Hoffman,Christoph Hafemeister,Efthymia Papalexi,William M. Mauck,Yuhan Hao,Marlon Stoeckius,Peter Smibert,Rahul Satija +9 more
TL;DR: A strategy to "anchor" diverse datasets together, enabling us to integrate single-cell measurements not only across scRNA-seq technologies, but also across different modalities.
Journal ArticleDOI
An RNA-Sequencing Transcriptome and Splicing Database of Glia, Neurons, and Vascular Cells of the Cerebral Cortex
Ye Zhang,Kenian Chen,Steven A. Sloan,Mariko L. Bennett,Anja R. Scholze,Sean O'Keeffe,Hemali Phatnani,Paolo Guarnieri,Christine Caneda,Nadine Ruderisch,Shuyun Deng,Shane A. Liddelow,Chaolin Zhang,Richard Daneman,Tom Maniatis,Ben A. Barres,Jian Qian Wu +16 more
TL;DR: The authors' data provide clues as to how neurons and astrocytes differ in their ability to dynamically regulate glycolytic flux and lactate generation attributable to unique splicing of PKM2, the gene encoding the glycoleytic enzyme pyruvate kinase.
Journal ArticleDOI
A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function.
John D. Cahoy,Ben Emery,Amit Kaushal,Lynette C. Foo,Jennifer L. Zamanian,Karen S. Christopherson,Yi Xing,Jane L. Lubischer,Paul A. Krieg,Sergey A. Krupenko,Wesley J. Thompson,Ben A. Barres +11 more
TL;DR: These findings call into question the concept of a “glial” cell class as the gene profiles of astrocyte and oligodendrocytes are as dissimilar to each other as they are to neurons, for better understanding of neural development, function, and disease.
Journal ArticleDOI
RNA velocity of single cells
Gioele La Manno,Gioele La Manno,Ruslan A. Soldatov,Amit Zeisel,Amit Zeisel,Emelie Braun,Emelie Braun,Hannah Hochgerner,Hannah Hochgerner,Viktor Petukhov,Viktor Petukhov,Katja Lidschreiber,Maria Eleni Kastriti,Peter Lönnerberg,Peter Lönnerberg,Alessandro Furlan,Jean Fan,Lars E. Borm,Lars E. Borm,Zehua Liu,David van Bruggen,Jimin Guo,Xiaoling He,Roger A. Barker,Erik Sundström,Gonçalo Castelo-Branco,Patrick Cramer,Patrick Cramer,Igor Adameyko,Sten Linnarsson,Sten Linnarsson,Peter V. Kharchenko +31 more
TL;DR: It is shown that RNA velocity—the time derivative of the gene expression state—can be directly estimated by distinguishing between unspliced and spliced mRNAs in common single-cell RNA sequencing protocols, and expected to greatly aid the analysis of developmental lineages and cellular dynamics, particularly in humans.
Journal ArticleDOI
The glial nature of embryonic and adult neural stem cells
TL;DR: The timing in development and location of NSCs, a property tightly linked to their neuroepithelial origin, appear to be the key determinants of the types of neurons generated.