Developmental Regulation of α-Mannosidase in Dictyostelium discoideum
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TLDR
Control of the period of synthesis by the overall developmental program is demonstrated in two temporally deranged morphological mutants and studies with preferential inhibitors of macromolecular synthesis indicate that accumulation of alpha-mannosidase requires concomitant protein synthesis and prior ribonucleic acid synthesis.Abstract:
The specific activity of α-mannosidase (EC 3.2.1.24) has been found to increase more than a thousandfold during development of the cellular slime mold, Dictyostelium discoideum. The enzyme accumulates in both spore and stalk cells. Studies with preferential inhibitors of macromolecular synthesis indicate that accumulation of α-mannosidase requires concomitant protein synthesis and prior ribonucleic acid synthesis. Control of the period of synthesis by the overall developmental program is demonstrated in two temporally deranged morphological mutants. α-Mannosidase is found in lysosomes of D. discoideum in association with other acid hydrolases which may be involved in metabolism of extracellular polysaccharide.read more
Citations
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Journal ArticleDOI
Characterization of the genome of the cellular slime mold Dictyostelium discoideum.
Richard A. Firtel,James Bonner +1 more
TL;DR: The cellular DNA's of Dictyostelium discoideum have been characterized by their behavior in CsCl buoyant density-gradient centrifugation and by their renaturation kinetics and the complexity of the nuclear DNA is but 11 times that of Escherichia coli.
Journal ArticleDOI
Density-dependent induction of discoidin-I synthesis in exponentially growing cells of Dictyostelium discoideum
TL;DR: The findings suggest that the expression of several 'early developmental' genes is regulated by a mechanism that measures cell density relative to food supply, not by starvation per se.
Journal ArticleDOI
Secretion of Lysosomal enzymes in the cellular slime mold, Dictyostelium discoideum.
TL;DR: It is shown that the egestion of indigestible material from residual or terminal phagosomes is kinetically distinct from the secretion of any of the lysosomal enzymes, which indicates that the secreted acid phosphatase activity may be localized within different vesicles than the above glycosidases.
Journal ArticleDOI
Changes in the expression of single-copy DNA during development of the cellular slime mold Dictyostelium discoideum.
TL;DR: Ritonucleic acid extracted from four stages during the developmental cycle of the cellular slime mold Dictyostelium discoideum was hybridized with purified single-copy nuclear DNA, indicating that there are, during development, qualitative and quantitative changes in the portion of the single- copies of the genome transcribed.
Journal ArticleDOI
Isolation of mutations in Dictyostelium discoideum affecting α-mannosidase
S.J. Free,William F. Loomis +1 more
TL;DR: In this paper, a minor isozyme, α-mannosidase-2, was recognized which accumulates only during culmination and makes up about 10 p. cent of the total activity in fruiting bodies.
References
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Hans Lineweaver,Dean Burk +1 more
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
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Book ChapterDOI
Chapter 14 Biochemical and Genetic Methods in the Study of Cellular Slime Mold Development
TL;DR: This chapter describes genetic, biochemical, and immunochemical techniques that have been successfully applied in the laboratory to the study of cellular slime mold development.
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