Diagnosis of Mycoplasma hyopneumoniae.
TL;DR: The use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories as mentioned in this paper.
Abstract: Mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. This small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. Confirming the presence of M. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. While culture of the organism remains the gold standard for identification, the use of serology, the polymerase chain reaction and various assays to detect the presence of M. hyopneumoniae in tissue is common in diagnostic laboratories. Because of the role M. hyopneumoniae plays in increasing the severity of pneumonia associated with concurrent bacterial and viral infections, understanding the pathogenesis and diagnostic assays available is critical for developing effective intervention strategies to control respiratory disease on a herd basis.
Citations
More filters
TL;DR: The main effects of vaccination include less clinical symptoms, lung lesions and medication use, and improved performance, however, bacterins provide only partial protection and do not prevent colonization of the organism.
370 citations
TL;DR: Lung samples from 148 finishing pigs with cranioventral lobular bronchopneumonia consistent with porcine respiratory disease complex (PRDC) in Denmark revealed a broad range of microscopical lesions and the diversity and number of pathogens were higher in these animals compared with controls.
172 citations
TL;DR: In this review, the characteristics of M. hyopneumoniae related to pathogenesis and control measures will be discussed and special emphasis will be placed on vaccination strategies that have been proposed with the use of reverse vaccinology approaches.
75 citations
TL;DR: It is indicated that vaccination does not significantly reduce the transmission of this respiratory pathogen in nursery pigs under field conditions.
Abstract: This study investigated the effect of vaccination against Mycoplasma hyopneumoniae on its transmission in nursery pigs under field conditions. Seventy-two pigs were randomly allocated at weaning into vaccinated (V) and non-vaccinated (NV) groups. Animals in the V group were vaccinated at 3 weeks of age with a commercial M. hyopneumoniae bacterin vaccine. Broncho-alveolar lavage fluid taken at weaning and at the end of the nursery period was assessed for the presence of M. hyopneumoniae by nested PCR, and the reproduction ratio of infection (Rn) was calculated. The percentage of positive pigs in the V and NV groups was 14% and 36% at weaning, and 31% and 64% at the end of the nursery period, respectively. The Rn-values for the V and NV groups were 0.71 and 0.56, respectively (P > 0.05). The study indicates that vaccination does not significantly reduce the transmission of this respiratory pathogen.
62 citations
TL;DR: A proteomic analysis, based on two-dimensional gel electrophoresis of soluble protein extracts, immunoblot and mass spectrometry, which was carried out aiming the identification of gene products and antigenic proteins from the M. hyopneumoniae pathogenic strain 7448 produced a proteome map that is expected to serve as a reference for comparative analyses for methabolic studies based on cells cultured under modified conditions.
62 citations
References
More filters
TL;DR: A species-specific polymerase chain reaction (PCR)-based diagnostic reagent for the identification of Mhp was developed based upon DNA sequence analysis of a cloned fragment of MHP DNA.
41 citations
Journal Article•
TL;DR: This work reviews the interactions among the different microorganisms involved and the immunological strategies utilized to control this disease and finds that protection was not complete and colonization by M. hyopneumoniae was not prevented, as is claimed to have been the case with other vaccines.
19 citations
11 citations